LOCUS       sequence-29162-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Ibarra JA, Knodler LA, Sturdevant DE, Virtaneva K, Carmody AB, 
            Fischer ER, Porcella SF, Steele-Mortimer O
  TITLE     Induction of Salmonella pathogenicity island 1 under different 
            growth conditions can affect Salmonella-host cell interactions in 
            vitro.
  JOURNAL   Microbiology (Reading). 2010 Apr;156(Pt 4):1120-1133. doi: 
            10.1099/mic.0.032896-0. Epub 2009 Dec 24.
  PUBMED    20035008
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology (Reading)."; date: "2010-04"; volume: "156(Pt 
            4):1120-1133. doi"; pages: " 10.1099/mic.0.032896-0. Epub 2009 Dec 
            24"
COMMENT     Sequence Label: pMPMA3dPlac-null-GFPLVA
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..61
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     45..61
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     RBS             139..150
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(187..215)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgactactat gggcgaattg gagctccacc gcggtggcgg ccgctctaga actagtggat
       61 cccccgggct gcaggaattc gatatcaagc ttgcatgcct gcaggtcgac tctagaggat
      121 ccccgggtac ctagaattaa agaggagaaa ttaagcatgc gtaaaggaga agaacttttc
      181 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      241 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      301 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
      361 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
      421 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
      481 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
      541 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
      601 aatgtataca tcatggcaga caaacaaaag aatggaatca aagtttaact tcaaaattag
      661 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactcccat
      721 tggggatggc
//
