LOCUS       sequence-291625-        4762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 nsp11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4762)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046673
FEATURES             Location/Qualifiers
     source          1..4762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..417
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(517..535)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             886..1689
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      1772..2754
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      2832..2861
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      2883..3470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(3482..3509)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3533..3724)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(3725..4516,4517..4585))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4348..4367
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4586..4688)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(4689..4720)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gcggccgccc cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat
       61 tttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat
      121 ttcccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa
      181 aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg
      241 tttctttttc ttgaaaattt ttttttttga tttttttctc tttcgatgac ctcccattga
      301 tatttaagtt aataaacggt cttcaatttc tcaagtttca gtttcatttt tcttgttcta
      361 ttacaacttt ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt
      421 taattacaaa atgtccgctg acgcacaatc attcctaaat gggtttgctg tataaacagg
      481 ccccttttcc tttgtcgata tcatgtaatt agttatgtca cgcttacatt cacgccctcc
      541 tcccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat
      601 ttattttttt taatagttat gttagtatta agaacgttat ttatatttca aatttttctt
      661 ttttttctgt acaaagcggc cgccgaacca ctagtgcact gcagtacacg gtttccttga
      721 aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt
      781 agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta
      841 acccaactgc acagaacaaa aacgtgcagg aaacgaagat aaatcatgtc gaaagctaca
      901 tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg
      961 cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg
     1021 gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg
     1081 actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat
     1141 tttttactct tcgaggacag aaaatttgct gacattggta atacagtcaa attgcagtac
     1201 tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg
     1261 ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga
     1321 ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact
     1381 aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa
     1441 agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt
     1501 ttagatgaca agggagatgc attgggtcaa cagtatagaa ccgtggatga tgtggtttct
     1561 acaggatctg acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag
     1621 gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag
     1681 caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca aattagagct
     1741 tcaatttaat tatatcagtt attacccgag taacgaagca tctgtgcttc attttgtaga
     1801 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
     1861 agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt
     1921 gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     1981 tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct
     2041 tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt
     2101 actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag
     2161 gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc
     2221 tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat
     2281 aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa
     2341 gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt
     2401 ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg
     2461 aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat
     2521 gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag
     2581 ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt
     2641 attcgcaata ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg
     2701 tcatcagagc gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagctagag
     2761 aataggaact tcccgagcgg ccgcgtgtta caagcttgac tccagcgtaa ctggactgca
     2821 atcaactcac tggctcacct tcacgggtgg gcctttcttc ggtagaaaat caaaggatct
     2881 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2941 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgag gtaactggct
     3001 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3061 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3121 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3181 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3241 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3301 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3361 agcttccagg ggtaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3421 ttgagcatcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3481 acgcagaaag gcccacccga aggtgagcca ggtgattaca tttgggccct catcagaggt
     3541 tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt
     3601 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa
     3661 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg
     3721 tcacttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3781 ccatagttgc ctgactcccc gtcgtgtaga taactacgat gcgggagggc ttaccatctg
     3841 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3901 taaaccagcc agccgggagg gccgagcgca gaagtgatcc tgcaacttta tccgcctcca
     3961 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4021 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4081 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4141 aagcggttag ctccttcggt cctccgatcg ttgccagaag taagttggcc gcagtgttat
     4201 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtgagatgct
     4261 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4321 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4381 tgctcatcat tggaaaacgt tcttcggggc gtaaactctc aaggatctta ccgctgttga
     4441 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4501 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4561 cgacacggaa atgttgaata ctcattttag cttccttagc tcctgaaaat ctcgataact
     4621 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     4681 gccgatcaag tcaaaagcct ccggtcggag gcttttgact ttctgctatg gaggtcaggt
     4741 atgatttaaa tggtcagtta ga
//
