LOCUS       sequence-29165-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Ibarra JA, Knodler LA, Sturdevant DE, Virtaneva K, Carmody AB, 
            Fischer ER, Porcella SF, Steele-Mortimer O
  TITLE     Induction of Salmonella pathogenicity island 1 under different 
            growth conditions can affect Salmonella-host cell interactions in 
            vitro.
  JOURNAL   Microbiology (Reading). 2010 Apr;156(Pt 4):1120-1133. doi: 
            10.1099/mic.0.032896-0. Epub 2009 Dec 24.
  PUBMED    20035008
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology (Reading)."; date: "2010-04"; volume: "156(Pt 
            4):1120-1133. doi"; pages: " 10.1099/mic.0.032896-0. Epub 2009 Dec 
            24"
COMMENT     Sequence Label: pMPMA3dPlac-PfliC-GFPLVA
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..63
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     47..63
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     RBS             439..450
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(487..515)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg
       61 atcccccggg ctgcaggaat tcgatatcaa gcttgcatgc ctgcaggtcg actctagaca
      121 gtagttaagc gcgttatcgg caatctggag gcaaagttta atgataattt tgcaaaaata
      181 atgcgcggaa taatgatgca taaagcggct atttcgccgc ctaagaaaaa gatcggggga
      241 agtgaaaaat tttctaaagt tcgaaattca ggtgccgata caagggttac ggtgagaaac
      301 cgtgggcaac agcccaataa catcaagttg taattgataa ggaaaagatc atggcacaag
      361 tcattaatac aaacagcctg tcgctgttga cccagaataa cctgaacaaa tcccagtccg
      421 ctctgggtac ctagaattaa agaggagaaa ttaagcatgc gtaaaggaga agaacttttc
      481 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      541 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      601 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
      661 caatgctttg cgagatacca gatcatatga aacagcatga cttttcagag tgc
//
