LOCUS       sequence-29168-e         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Ibarra JA, Knodler LA, Sturdevant DE, Virtaneva K, Carmody AB, 
            Fischer ER, Porcella SF, Steele-Mortimer O
  TITLE     Induction of Salmonella pathogenicity island 1 under different 
            growth conditions can affect Salmonella-host cell interactions in 
            vitro.
  JOURNAL   Microbiology (Reading). 2010 Apr;156(Pt 4):1120-1133. doi: 
            10.1099/mic.0.032896-0. Epub 2009 Dec 24.
  PUBMED    20035008
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology (Reading)."; date: "2010-04"; volume: "156(Pt 
            4):1120-1133. doi"; pages: " 10.1099/mic.0.032896-0. Epub 2009 Dec 
            24"
COMMENT     Sequence Label: pMPMA3dPlac-PpipB-GFPLVA
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..63
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     47..63
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     RBS             547..558
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(595..623)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg
       61 atcccccggg ctgcaggaat tcgatatcaa gcttgcatgc ctgcaggtcg actctagacg
      121 catttattct ggtataagtt gaaatactgc aaaaaatatt ggtgcttatt attttttctt
      181 taagtaaatt ttcgctcaac aaacttaatt gtttattcaa tgatgatgaa gcgtaagcta
      241 tgctggaaat gaaggaagtc aatagcaagg ataatcttat tattcacggg tgatattact
      301 tctgcttcac cgttatggca gatatcatcg cctcttgtca gatgccagac acctactcat
      361 actcaaccaa agctctaaat acaaaaatca ccttatatct ttttttatta ttccttgtat
      421 aaatgtgact tgactcacac ctataaggag tcggctcact tccataagaa ggaatcaaaa
      481 tgccaataac taacgcgtcc ccagaaaata tattaagata tttgcatgcg gccggtacct
      541 agaattaaag aggagaaatt aagcatgcgt aaaggagaag aacttttcac tggagttgtc
      601 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aa
//
