LOCUS       sequence-291721-       16400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of ORF1 (Shuffle 1 NLS) and Pong TPase (L418A, L420A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16400)
  AUTHORS   Johnson A, Mcassey E, Diaz S, Reagin J, Redd PS, Parrilla DR, Nguyen
            H, Stec A, McDaniel LAL, Clemente TE, Stupar RM, Parrott WA, Hancock
            CN
  TITLE     Development of mPing-based activation tags for crop insertional 
            mutagenesis.
  JOURNAL   Plant Direct. 2021 Jan 19;5(1):e00300. doi: 10.1002/pld3.300. 
            eCollection 2021 Jan.
  PUBMED    33506165
REFERENCE   2  (bases 1 to 16400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2021-01-19"; volume: "5(1):e00300. doi"; pages: " 
            10.1002/pld3.300. eCollection 2021 Jan"
COMMENT     Sequence Label: pWMD23
FEATURES             Location/Qualifiers
     source          1..16400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(27..734)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    787..811
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2213..2237)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4490..4870
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             4876..5427
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      5437..5689
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    5775..5799
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     mobile_element  6211..6978
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             7001..7795
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7882..8470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8371..8390
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8624..8641
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8656..8796
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8722..8741)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8882..8904
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9140..9334
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9400..10473)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10902..11531)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12832..12856
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(13118..13825)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     16371..16393
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(16385..16400,1..2)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(16386..16400,1..2)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtgccaagct tgcatgcctg caggtcctgc tgagcctcga catgttgtcg caaaattcgc
       61 cctggacccg cccaacgatt tgtcgtcact gtcaaggttt gacctgcact tcatttgggg
      121 cccacataca ccaaaaaaat gctgcataat tctcggggca gcaagtcggt tacccggccg
      181 ccgtgctgga ccgggttgaa tggtgcccgt aactttcggt agagcggacg gccaatactc
      241 aacttcaagg aatctcaccc atgcgcgccg gcggggaacc ggagttccct tcagtgagcg
      301 ttattagttc gccgctcggt gtgtcgtaga tactagcccc tggggcactt ttgaaatttg
      361 aataagattt atgtaatcag tcttttaggt ttgaccggtt ctgccgcttt ttttaaaatt
      421 ggatttgtaa taataaaacg caattgtttg ttattgtggc gctctatcat agatgtcgct
      481 ataaacctat tcagcacaat atattgtttt cattttaata ttgtacatat aagtagtagg
      541 gtacaatcag taaattgaac ggagaatatt attcataaaa atacgatagt aacgggtgat
      601 atattcatta gaatgaaccg aaaccggcgg taaggatctg agctacacat gctcaggttt
      661 tttacaacgt gcacaacaga attgaaagca aatatcatgc gatcataggc gtctcgcata
      721 tctcattaaa gcaggactct agggactagt cccgggtctt aattaactct ctagactcac
      781 ctaggcacca ctttgtacaa gaaagctggg tcttagtcag caaataactt ttcctccagc
      841 ttttgtaatg ccttgtctcg gcgagccttc tgttcttcag acatgttact tgtatcttga
      901 gtgagcaggg aattgtatac ctcaaacatc tttgcttctt tttgctgttc tgctgccttc
      961 aaatttacgt ttgaaatgtg aatctgtgct tcagtgacct tctcacgctc cagcttccga
     1021 tcttgcgtca ctttgataat tttctggaca ttgtccccta ggaggacaat gccttccata
     1081 acattttctt tcttgcgctt tcttttgcgc tcagactttg ctgcttctct accaatagga
     1141 cgtttctgct gttctggaac agcatccatt tcgctcttcc ttttcctctt ttcaaactga
     1201 gcacaccatt tgggctctct tttgagtatc ttccaccaat ggaccaacgc aaaaggtttt
     1261 ccaaacctgt ttgcatacag cctctgtgcc tctttctcaa gcatgtcgtc tgagtatccg
     1321 cttgtatgca tttgagtaac cgtactccaa tagtcattga actcagagat cgctgacttc
     1381 aaccttgacc agtgaacctt cagttggtta atttccctcc tacgttttcc attccctttc
     1441 ttgttaaatt catcagtgac ttccttccaa aatgtatcac ctttcttatc attcccatga
     1501 attgagtctt tagaagcatt caaccaagca ctggccagtc tctcttcctc ttcatgagtc
     1561 catcttttct ttgctgccct actgtcatca ttgttgtcct cttcatcaat cataatgttt
     1621 gttgaagtgc cttggttcgt agcttggggt gttggtgttc cgtaggcact tggtggctgt
     1681 ggagatattg ggttgaaccc catattgtga ccaacgaaat gaaaattttc accttgctgt
     1741 tgtggctgcg gcggacaatt ttgttgaata aaattgacaa accctcctgg aggataaccc
     1801 ctgaccaaac atgaatttgg gtcttgttgg tttccaaaga aagatgcaaa gtttggggaa
     1861 atggtcgatg ttggtcccca tcctcctcct tgtggttgcg gtggccttgc cgtcgatgtc
     1921 cccgaagcaa gagaggaggt cggcgcagcc ggtgacggat tcggcgaatc ggggacgaaa
     1981 atcggcgaag attgcggtgg tcgtggtggg atggccgtcg gcgcagcagg ggatgaagac
     2041 gtcgcagcag gaggcggggt ggccgggaca gaaatcggcg gcggcctctt caatcctagt
     2101 tgcttgcccc cgcgctgttt gcctcctttc ccggttgcac gccgccgggt ttcagcagcc
     2161 ggcgacggat ccacggccgg cgacggatcc acggccggcg acggatccat gaagcctgct
     2221 tttttgtaca aacttgtgat ctcgatccgg ctgtggtgag agaaacagag cgtgagctca
     2281 aatacacaag tacgaaatca gagttatgag agaggatttg gagaaagaag atcacctgaa
     2341 gatcgtttgc gtcgcgcgaa tgagagagac ggcagagaaa acgagaagct ttaagaggac
     2401 gaacaaaaac cctaatgtca ctccttttat atagatgaga aatttagggt ttgggctcta
     2461 atggagctgt ctacatgggc tttcatttct ttatccagta aagaaccggt ttaacaggtt
     2521 ccctggttta tacgtggttt tagccggtta acttacggaa tattattttt atcttaagtt
     2581 acacaaatta catcattaat agtaaaacaa aattttatgg tgcaatttaa gtttttatga
     2641 tgagtgaaat tcgaataata tacaaatatt ttactgttta ggtttcccta agcctacttt
     2701 tagatatgta aattaaacat tttttttttt ttggttagaa ccaagtcagt gatcatagaa
     2761 ctcaaaaagc tggtgtttca attggtacaa gcatttttat tgcaagatat aaaaattcag
     2821 aaggacatat ccttgatgta tgtacatgaa ggaaccgatt ttgactccaa gctacaaaga
     2881 agaagaagaa tgatatacaa agaaaactgc atatcctcac catgaaattg aaagctagag
     2941 gaaggggaat aacagatact ctgcgtagaa gagattccat ataaaccgat aatatcctga
     3001 gatagcttcc tgcaataatc aaatgtttct acactccgtt agatatacac tccattgatg
     3061 ttccgtgata acgagtttat gtttgtacaa aagtatttta ccttggtgta gtttgctttt
     3121 tctagtaccc atgtctacca aagatcaaca acaaagatat tagagattaa tgaatttggc
     3181 agtgtcaaat gcagaaacta aagcatatag ttatgaagtt cttgtgcatt atctcaaaag
     3241 tatgtaagag atataagagt atctcataat gctttacctg gaaaattaag cctgaagcca
     3301 agatcacatg tgcaggaatc atcaagctac ctctaaaaac ctgttcattg ttaaaggata
     3361 agagggttgg tcttttcaaa gctgcatact gaggaaagta acaatatcgc aacaaaaata
     3421 aattggtggc gatagtttat caagcttcta catgcagtaa tggtgttcgt gctttaatta
     3481 accgaaatag ttaagacgga aatagatgaa ttcacctgag gcatgtagaa agctagtgat
     3541 atggctgaag cataatttac tagcagaagt ccagaaccga ggaatgcaat gtttctcact
     3601 ccaagttttg ttgccagggt tgatatttgg aacctattca agatcaatca aaggcatgat
     3661 taactaaaca tgcttgaaca aacacataaa gacaatatat caaacggagc tggttaaaca
     3721 ttgtctttga ctgtcaaatg gattgagcca aagagcggaa agaaaatggt tatgcgtgca
     3781 gttacttact ttcgatctcc ttcaacatca ggaaggtcct ttgtaatagc aatgaccagt
     3841 gcaaacagtg ccacaaaaga tgtgatgaac gccacaggtg cactgcaaat agcgaatcaa
     3901 aagttgagta atatatctac tcacgaacta gcctctctaa ccatctgtgg gtcagcattc
     3961 tttctgaaat tgaagaggct aaccttctca ttatcagtgg tgaacatagt gtcgtcacct
     4021 tcaccttcga acttccttcc tagatcgtaa agatagagga aatcgtccat tgtaatctcc
     4081 ggggcaaagg agatctcttt tggggctgga tcactgctgg gccttttggt tcctagcgtg
     4141 agccagtggg ctttttgctt tggtgggctt gttagggcct tagcaaagct cttgggcttg
     4201 agttgagctt ctcctttggg gatgaagttc aacctgtctg tttgctgact tgttgtgtac
     4261 gcgtcagctg ctgctcttgc ctctgtaata gtggcaaatt tcttgtgtgc aactccggga
     4321 acgccgtttg ttgccgcctt tgtacaaccc cagtcatcgt atataccggc atgtggaccg
     4381 ttatacacaa cgtagtagtt gatatgaggg tgttgaatac ccgattctgc tctgagagga
     4441 gcaactgtgc tgttaagctc agatttttgt gggattggaa ttctgagatt tttcaaatca
     4501 gtgcgcaaga cgtgacgtaa gtatccgagt cagtttttat ttttctacta atttggtcgt
     4561 ttatttcggc gtgtaggaca tggcaaccgg gcctgaattt cgcgggtatt ctgtttctat
     4621 tccaactttt tcttgatccg cagccattaa cgacttttga atagatacgc tgacacgcca
     4681 agcctcgcta gtcaaaagtg taccaaacaa cgctttacag caagaacgga atgcgcgtga
     4741 cgctcgcggt gacgccattt cgccttttca gaaatggata aatagccttg cttcctatta
     4801 tatcttccca aattaccaat acattacact agcatctgaa tttcataacc aatctcgata
     4861 caccaaatcg aatcgatgag cccagaacga cgcccggccg acatccgccg tgccaccgag
     4921 gcggacatgc cggcggtctg caccatcgtc aaccactaca tcgagacaag cacggtcaac
     4981 ttccgtaccg agccgcagga accgcaggag tggacggacg acctcgtccg tctgcgggag
     5041 cgctatccct ggctcgtcgc cgaggtggac ggcgaggtcg ccggcatcgc ctacgcgggc
     5101 ccctggaagg cacgcaacgc ctacgactgg acggccgagt cgaccgtgta cgtctccccc
     5161 cgccaccagc ggacgggact gggctccacg ctctacaccc acctgctgaa gtccctggag
     5221 gcacagggct tcaagagcgt ggtcgctgtc atcgggctgc ccaacgaccc gagcgtgcgc
     5281 atgcacgagg cgctcggata tgccccccgc ggcatgctgc gggcggccgg cttcaagcac
     5341 gggaactggc atgacgtggg tttctggcag ctggacttca gcctgccggt accgccccgt
     5401 ccggtcctgc ccgtcaccga gatttgactg cagaatcttg gactcccatg ttggcaaagg
     5461 caaccaaaca aacaatgaat gatccgctcc tgcatatggg gcggtttgag tatttcaact
     5521 gccatttggg ctgaattgaa gacatgctcc tgtcagaaat tccgtgatct tactcaatat
     5581 tcagtaatct cggccaatat cctaaatgtg cgtggcttta tctgtctttg tattgtttca
     5641 tcaattcatg taacgtttgc ttttcttatg aattttcaaa taaattatca gatccgagct
     5701 cgaattaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     5761 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     5821 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     5881 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     5941 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     6001 gattgtaacg atgacagagc gttgctgcct gtgatcaccg cggtttcaaa atcggctccg
     6061 tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt
     6121 taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt
     6181 ggggtatctt taaatactgt agaaaagagg ggtaatgact ccaacttatt gatagtgttt
     6241 tatgttcaga taatgcccga tgactttgtc atgcagctcc accgattttg agaacgacag
     6301 cgacttccgt cccagccgtg ccaggtgctg cctcagattc aggttatgcc gctcaattcg
     6361 ctgcgtatat cgcttgctga ttacgtgcag ctttcccttc aggcgggatt catacagcgg
     6421 ccagccatcc gtcatccata tcaccacgtc aaagggtgac agcaggctca taagacgccc
     6481 cagcgtcgcc atagtgcgtt caccgaatac gtgcgcaaca accgtcttcc ggagactgtc
     6541 atacgcgtaa aacagccagc gctggcgcga tttagccccg acatagcccc actgttcgtc
     6601 catttccgcg cagacgatga cgtcactgcc cggctgtatg cgcgaggtta ccgactgcgg
     6661 cctgagtttt ttaagtgacg taaaatcgtg ttgaggccaa cgcccataat gcgggctgtt
     6721 gcccggcatc caacgccatt catggccata tcaatgattt tctggtgcgt accgggttga
     6781 gaagcggtgt aagtgaactg cagttgccat gttttacggc agtgagagca gagatagcgc
     6841 tgatgtccgg cggtgctttt gccgttacgc accaccccgt cagtagctga acaggaggga
     6901 cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag
     6961 taagttggca gcatcaccga aaagaggaag gaaataataa atggctaaaa tgagaatatc
     7021 accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc
     7081 tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga
     7141 cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct
     7201 ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa
     7261 tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag
     7321 ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat
     7381 atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg attacttact
     7441 gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga
     7501 tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc
     7561 ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat
     7621 tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc
     7681 gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat
     7741 caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct
     7801 agaatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7861 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7921 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7981 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     8041 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8101 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8161 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8221 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8281 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8341 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8401 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8461 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8521 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8581 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8641 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     8701 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     8761 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     8821 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     8881 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt
     8941 gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag
     9001 attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga
     9061 agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc
     9121 tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt
     9181 taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg
     9241 tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta
     9301 cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc
     9361 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc
     9421 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc
     9481 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa
     9541 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc
     9601 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc
     9661 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc
     9721 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt
     9781 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt
     9841 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc
     9901 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc
     9961 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat
    10021 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat
    10081 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc
    10141 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact
    10201 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt
    10261 gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat
    10321 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg
    10381 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat
    10441 ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca
    10501 gtgccattgc agggccggca ggcaacccag ccgcttacgc ctggccaacc gcccgttcct
    10561 ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct
    10621 cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg
    10681 cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt
    10741 cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc
    10801 tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag
    10861 cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta
    10921 atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga
    10981 acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca
    11041 agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct
    11101 ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc
    11161 tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc
    11221 ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc
    11281 gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc
    11341 gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg
    11401 gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg
    11461 cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg
    11521 ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag
    11581 cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct
    11641 cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg
    11701 ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg
    11761 ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct
    11821 ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc
    11881 ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc
    11941 ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc
    12001 ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg
    12061 ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct
    12121 ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa
    12181 caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc
    12241 cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag
    12301 gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc
    12361 cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc
    12421 ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc
    12481 atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc
    12541 gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag
    12601 ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg
    12661 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg
    12721 atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt
    12781 tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg
    12841 ccaatatatc ctgtcaaaca ctgatagttt acttctgatc ttgagaaata tatctttctc
    12901 tgtgttcttg atgcagttag tcctgaatct tttgactgca tctttaacct tcttgggaag
    12961 gtatttgatt tcctggagat tattgctcgg gtagatcgtc ttgatgagac ctgctgcgta
    13021 agcctctcta accatctgtg ggttagcatt ctttctgaaa ttgaaaaggc taatctgggg
    13081 acctgcaggg gcaatacatc ttgcatgcct gcaggtcctg ctgagcctcg acatgttgtc
    13141 gcaaaattcg ccctggaccc gcccaacgat ttgtcgtcac tgtcaaggtt tgacctgcac
    13201 ttcatttggg gcccacatac accaaaaaaa tgctgcataa ttctcggggc agcaagtcgg
    13261 ttacccggcc gccgtgctgg accgggttga atggtgcccg taactttcgg tagagcggac
    13321 ggccaatact caacttcaag gaatctcacc catgcgcgcc ggcggggaac cggagttccc
    13381 ttcagtgagc gttattagtt cgccgctcgg tgtgtcgtag atactagccc ctggggcact
    13441 tttgaaattt gaataagatt tatgtaatca gtcttttagg tttgaccggt tctgccgctt
    13501 tttttaaaat tggatttgta ataataaaac gcaattgttt gttattgtgg cgctctatca
    13561 tagatgtcgc tataaaccta ttcagcacaa tatattgttt tcattttaat attgtacata
    13621 taagtagtag ggtacaatca gtaaattgaa cggagaatat tattcataaa aatacgatag
    13681 taacgggtga tatattcatt agaatgaacc gaaaccggcg gtaaggatct gagctacaca
    13741 tgctcaggtt ttttacaacg tgcacaacag aattgaaagc aaatatcatg cgatcatagg
    13801 cgtctcgcat atctcattaa agcaggactc tagattagaa tgttaattaa tcaatttcca
    13861 gttctatgtg cagcaccacc aaacttattc caaatgtgtt ccaccaaatc tttcttaagt
    13921 cggttatgag ccgctcgatc tcggatagaa atatcttttt ctaaaactct atcaaatggt
    13981 gtgttctgtt caggagagaa ctcaggttcc tgaacggttg atgaactagg aggcacattt
    14041 gcatctgcat cttcttcaat aattctggtt tccttctcat cttcaactat catattgtga
    14101 agtatgatgc aagctagaac aacatctcgc agtacacctc gatcatatag acgagctggt
    14161 cgttttaaga tgcaaaatct tcgctgcaat acaccaaagg ctctctcgat atcttttctt
    14221 gccccttctt gcatatctgc atacaatttc tccttttcag tgtttgggag tcgtattgac
    14281 ttaacaaaca ctgcccattc agggtagatt ccatcagcaa gaaaataccc agtattgtat
    14341 tgattcccat ttaccatgta ctggactcta ggagcttgtc ctttgagctc cttgataaat
    14401 acagtagatt ggttcaatac attgatatca ttgttggaac ccgctgctcc aaaaaatgca
    14461 tgccaaatcc aaagatcatg cgatgccaca gcctcaagaa tcagggttgg cactttctga
    14521 tctccacgag tgaactgacc cttccatgct actgggcatc tttcccaatg ccagtgcatg
    14581 cagtcaatgc tgccgaacat tccaggaaaa ccacgtttct caccaagttg gagaagccgt
    14641 tcggtatctt ccatggtggg gcgcctaaga tacctctcac caaacacatc ttgaagacct
    14701 ttgacaaaat tcttcattgc ctccattgct gtagtctctc ctatcttcag atattcatct
    14761 agttcatctg cgccactacc agtagccaac tggcgaatag ctgcagtaca cttttgcagt
    14821 ggactgagtc ctttccgatt aacagcatcc accctttgtg tgaaatacac tgaccactgg
    14881 cctaatgcct caacgatgcg aagaaaaagt ggcctagaca tacgaaatct tcgacgaaaa
    14941 attttggaag ggtaaagagg attttctgaa aagtaatcat tcactagttg ctgatgtgct
    15001 tcctctcgtg gcctcttgat gtgcttcctc ggatgatgtc gatggtcaga agcctcgttt
    15061 tgaagacctt ctatagtgga ctgtactacc accgtggctt cagtgagaag atcatctatg
    15121 atctcatcct cagccaggaa ctcatccaac aagttcactg gaacttgact tccatcagta
    15181 ttttgctcag acatctttga agaagagaga aacagtaaag ataaaaggga ggaggtcttc
    15241 gtatgagaaa agagggaatg agtttctgag aggagtagag agatggctaa actctgcatg
    15301 gcgcgccgtt tgaaggtacc tgtcgagtca acaatcacag ataaatcaga atcaaaatcc
    15361 attacgtaat cttaagtacg gatctgaagc aatctcgtga aaaaaaaccc taatccacgg
    15421 caatagaatc aacaaacaag gcagttgcta acagatctga gcaatatctc tcgaaaaacg
    15481 atatagaatc aacgaataag gctttttgtc aacggatctg aacaatttct tgaaaaacca
    15541 cagaagtata aaaaataagg ctgttgttta acggatctga agtaattcat gaaaaagccc
    15601 tgatctatgg ttacgtttaa cggatctgaa gtaatcaatg aaaaaagccc taatctacga
    15661 ttaatgaatc aacaattaag gcttgttgta aagggatctg acatgaaaaa gctctaatta
    15721 gagcaaatct acgattaaag aatcatcaaa ttaaggacgt tgttgtaaag ataataaaag
    15781 ctgaatctga ttatgaaaat tgaagatcga tgaaaccaaa caaaacacaa tcaaatcaac
    15841 atcgtattga tcaaagaaca tacacaattc atggaaagaa cgaagaagaa aaacagaaga
    15901 aatcgactaa ccttgaaggg agaggtaggg aaatacacag agagaatgga atatattgga
    15961 attgaactgc gaaggaagga ggaggggtgt ggggcgtttt atagcataag gtgtggaacc
    16021 cttctccagt catattgtga cgcgtgttca tgtatggtgt cacttggtgc ctatgacacg
    16081 ggtaatattt ggtattggat gcattttcgg agagtccttc taggaaattg agaattgcaa
    16141 tttgcgtggt agttggcggt ttcgtaatct tgacggagtc acatgcacct tcaagctaaa
    16201 ctgaaggcgg gaaacgacaa tctgatccaa gctcaagctg ctctagcatt cgccattcag
    16261 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    16321 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    16381 acgttgtaaa acgacggcca
//
