LOCUS       sequence-291734-        9967 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Light-induced recruitment of QPAS1-mCherry to the target protein 
            bound to membrane; interaction occurs via intrabody fused to BphP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Redchuk TA, Karasev MM, Verkhusha PV, Donnelly SK, Hulsemann M, 
            Virtanen J, Moore HM, Vartiainen MK, Hodgson L, Verkhusha VV
  TITLE     Optogenetic regulation of endogenous proteins.
  JOURNAL   Nat Commun. 2020 Jan 30;11(1):605. doi: 10.1038/s41467-020-14460-4.
  PUBMED    32001718
REFERENCE   2  (bases 1 to 9967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-30"; volume: "11(1):605. doi"; pages: " 
            10.1038/s41467-020-14460-4"
COMMENT     Sequence Label: pQP-2460
FEATURES             Location/Qualifiers
     source          1..9967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        102..405
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        406..609
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     560..580
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(644..646,647..649,650..1360)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     ERVTAAGITLGMDELYK"
     primer_bind     complement(689..710)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(950..969)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    1415..1471
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    1473..2046
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1496..2048
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     regulatory      2079..2088
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4346..4687
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
     misc_feature    4697..4753
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    4755..5329
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4779..5331
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             5388..6095
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5533..5551)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5801..5820
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6600..6614
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     polyA_signal    6796..6917
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6833..6852)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6887..6906
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6924..7379)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7061..7082)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7273..7292
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7406..7510
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7512..7869
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7512..7703
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7535..7555)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7720..7855
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7904..8698
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7904..8698
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7958..7977)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8568..8587
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8886..8905)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8930..8977
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9306..9894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9795..9814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca
       61 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      121 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      181 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      241 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      301 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      361 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      421 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      481 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      541 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      601 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accatggtga gcaagggcga
      661 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      721 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      781 gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg
      841 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
      901 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      961 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1021 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1081 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     1141 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa
     1201 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctaa gctaccagtc
     1261 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg aacgcgtgac
     1321 cgccgccggg atcactctcg gcatggacga gctgtacaag gctagcggta aaaagaagaa
     1381 aaagaagtca aagacaaagt gtgtaattat gtaaagatct cgagctcaag cttcgaattc
     1441 tgcagtcgac ggtaccgcgg gcccgggatc cgcccctctc cctccccccc ccctaacgtt
     1501 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     1561 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     1621 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     1681 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     1741 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     1801 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     1861 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     1921 cattgtatgg gatctgatct ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg
     1981 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     2041 tgataatatg gccacaagcg ctaccggact cagatctacc atggtggcag gtcatgcctc
     2101 tggcagcccc gcattcggga ccgccgatct ttcgaattgc gaacgtgaag agatccacct
     2161 cgccggctcg atccagccgc atggcgcgct tctggtcgtc agcgagccgg atcatcgcat
     2221 catccaggcc agcgccaacg ccgcggaatt tctgaatctc ggaagcgtgc tcggcgttcc
     2281 gctcgccgag atcgacggcg atctgttgat caagatcctg ccgcatctcg atcccaccgc
     2341 cgaaggcatg ccggtcgcgg tgcgctgccg gatcggcaat ccctccacgg agtacgacgg
     2401 tctgatgcat cggcctccgg aaggcgggct gatcatcgag ctcgaacgtg ccggcccgcc
     2461 gatcgatctg tccggcacgc tggcgccggc gctggagcgg atccgcacgg cgggctcgct
     2521 gcgcgcgctg tgcgatgaca ccgcgctgct gtttcagcag tgcaccggct acgaccgggt
     2581 gatggtgtat cgcttcgacg agcagggcca cggcgaagtg ttctccgagc gccacgtgcc
     2641 cgggctcgaa tcctatttcg gcaaccgcta tccgtcgtcg gacattccgc agatggcgcg
     2701 gcggctgtac gagcggcagc gcgtccgcgt gctggtcgac gtcagctatc agccggtgcc
     2761 gctggagccg cggctgtcgc cgctgaccgg gcgcgatctc gacatgtcgg gctgcttcct
     2821 gcgctcgatg tcgccgatcc atctgcagta cctgaagaac atgggcgtgc gcgccaccct
     2881 ggtggtgtcg ctggtggtcg gcggcaagct gtggggcctg gttgcctgtc accattatct
     2941 gccgcgcttc atccatttcg agctgcgggc gatctgcgaa ctgctcgccg aagcgatcgc
     3001 gacgcggatc accgcgcttg agagcttcgc gcagagccag tcggagctgt tcgtgcagcg
     3061 gctcgaacag cgcatgatcg aagcgatcac ccgtgaaggc gattggcgcg cagcgatttt
     3121 cgacaccagc caatcgatcc tgcagccgct gcacgccgac ggttgcgcgc tggtgtacga
     3181 agaccagatc aggaccatcg gtgacgtacc ttccacgcag gatgttcgcg agatcgccgg
     3241 gtggctcgat cgccagccac gtgcggcggt gacctcgacc gcgtcgctcg gtctcgacgt
     3301 gccggagctc gcgcatctga cgcggatggc gagcggcgtg gtcgcggcgc cgatttcgga
     3361 tcatcgcggc gagtttctga tgtggttccg ccccgagcgc gtccacaccg ttacctgggg
     3421 cggcgatccg aagaagccgt tcacgatggg cgatacaccg gcggatctgt cgccgcggcg
     3481 ctccttcgcc aaatggcatc aggttgtcga aggcacgtcc gatccgtgga cggccgccga
     3541 tctcgccgcg gctcgcacca tcggtcagac cgtcgccgac atcgtgctgc aattccgcgc
     3601 ggtgcggaca ctgatcgccc gcgaacagta cgaacagttt tcgtcccagg tgcacgcttc
     3661 gatgcagccg gtgctgatca ccgacgccga aggccgcatc ctgctgatga acgactcgtt
     3721 ccgcgacatg ttgccggcgg ggtcgccatc cgccgtccat ctcgacgatc tcgccgggtt
     3781 cttcgtcgaa tcgaacgatt tcctgcgcaa cgtcgccgaa ctgatcgatc acggccgcgg
     3841 gtggcgcggc gaagttctgc tgcgcggcgc aggtaatcgc ccgttgccgc tggcagtgcg
     3901 cgccgatccg gtgacgcgca cggaggacca gtcgctcggc ttcgtgctga tcttcagcga
     3961 cgctaccgat cgtcgcaccg cagatgccgc acgcacgcgt ttccaggaag gcattcttgc
     4021 cagcgcacgt cccggcgtgc ggctcgactc caagtccgac ctcttgcacg agaagctgct
     4081 gtccgcgctg gtcgagaacg cgcagcttgc cgcattggaa attacttacg gcgtcgagac
     4141 cggacgcatc gccgagctgc tcgaaggcgt tcgccagtcg atgctgcgca ccgccgaagt
     4201 gctcggccat ctggtgcagc acgcggcgcg cacggccggc agcgacagct cgagcaatgg
     4261 ctcgcagaac aagaaggcta gcagtgctgg cggtagtgct ggtgggagtg ctggcggtag
     4321 tgctggtggc gatatcatgg atcaagtcca actggtggag tctggtggcg ctttggtgca
     4381 gccaggtggc tctctgcgtt tgtcctgtgc cgcttctggc ttcccagtga accgctattc
     4441 catgcgctgg tatcgccagg ctccaggcaa agagcgtgag tgggtagccg gtatgtccag
     4501 cgcgggtgat cgtagctcct atgaagactc cgtgaagggc cgtttcacca tcagccgtga
     4561 cgatgcccgt aacacggtgt atctgcaaat gaacagcttg aaacctgaag atacggccgt
     4621 gtattactgt aatgtgaacg tgggcttcga gtattggggc caaggcaccc aggtcaccgt
     4681 ctccagctaa gatatcagat ctcgagctca agcttcgaat tctgcagtcg acggtaccgc
     4741 gggcccggga tccgcccctc tccctccccc cccccctaac gttactggcc gaagccgctt
     4801 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     4861 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     4921 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     4981 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     5041 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     5101 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     5161 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     5221 tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     5281 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     5341 accggtccat gggaattgat ctctcaggat taacactgca gactagtatg gtgagcaagg
     5401 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     5461 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     5521 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     5581 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     5641 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     5701 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     5761 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     5821 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     5881 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     5941 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     6001 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     6061 gccactccac cggcggcatg gacgagctgt acaagagtgc tggtgggtcc gccggcggga
     6121 gtgctggcgg tagtgccggc ggtagttcta gaggcaagaa catgcaggcg gtcaccgagc
     6181 tgcattcccg gctgatcgct gcgcagcagg cgatggagcg cgactattgg cggttgcgtg
     6241 aattggagac tcgctaccgc ctggtgttcg acgctgccgc cgatgcggtg atgatcgtct
     6301 ccgccggcga catgcgcatc gtcgaagcca accgggcggc ggtgaatgcg atcagccgcg
     6361 tcgagcgcgg caatgacgac cttgcggggc gtgatttcct cgccgaagtg gcggctgccg
     6421 atcgcgatgc ggtgcgcgac atgctggccc aggtgcgtca gcgcggcacc gcactcagcg
     6481 tcctcgttca tctcggccgt tacgaccgcg cctggatgct gcgcggttcg ctgatgtcgt
     6541 ccgagcgtcg tcaggttttc ctgctgcact tcaccccggt gaccacgact cccgcgatcg
     6601 acgacgacga caaaggtgtc gttgcttctg ctgccgacgg tgcagagggc gcctcagacg
     6661 acgcagagga ttaagcggcc gcgactctag atcataatca gccataccac atttgtagag
     6721 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     6781 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6841 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6901 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     6961 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     7021 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     7081 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     7141 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     7201 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     7261 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     7321 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     7381 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7441 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7501 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     7561 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7621 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7681 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7741 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7801 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     7861 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     7921 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     7981 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     8041 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     8101 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     8161 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     8221 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     8281 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     8341 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     8401 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     8461 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     8521 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     8581 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     8641 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     8701 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     8761 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     8821 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     8881 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     8941 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     9001 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     9061 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     9121 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     9181 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9241 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9301 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9361 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9421 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9481 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9541 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9601 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9661 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9721 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9781 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9841 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9901 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9961 cctgcgt
//
