LOCUS       sequence-291735-       13614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xflag-tagged CHEK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13614)
  AUTHORS   Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins 
            KA, Wang CC, Janes KA
  TITLE     Sporadic activation of an oxidative stress-dependent NRF2-p53 
            signaling network in breast epithelial spheroids and 
            premalignancies.
  JOURNAL   Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 
            10.1126/scisignal.aba4200.
  PUBMED    32291314
REFERENCE   2  (bases 1 to 13614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2020-04-14"; volume: "13(627). pii: 
            13/627/eaba4200. doi"; pages: " 10.1126/scisignal.aba4200"
COMMENT     Sequence Label: pSLIK_neo_3xflag_CHEK2
FEATURES             Location/Qualifiers
     source          1..13614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1606..1723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1780..1804
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    1844..1862
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1880..1898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1916..1934
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1952..1970
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        1982..2020
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2017..2041
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2053..2118
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3792..3816)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3866..5077
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4914..4933
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5136..5753,5754..5759,5760..5795,5796..5798,5799..5834,
                     5835..5837,5838..5873,5874..5882)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5920..6490
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6084..6101)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6311..6330
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6506..7300
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6506..7300
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6560..6579)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7170..7189
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7468..8056
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7521..7541)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7939..7950)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8059..8075)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8060..8076)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8585..8765
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8791..8808)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8797..9021
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9067..9495
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9154..9173)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9364..9385
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9504..9524)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9509..9838
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9689..9824
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9751..9770
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9886..9933
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9952..10326
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10456..10577
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10493..10512)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10547..10566
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10626..10642)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10626..10642)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10639..10661)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10650..10666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10674..10680,10681..10698,10699..10704))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10719..10740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10857..10874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11028..11616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11108..11127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11787..12578,12579..12647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12410..12429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12648..12752)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12827..12846)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        13018..13397
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13399..13597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     1561 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1741 cagcagagat ccagtttggt taattctaga cagttatcaa caagtttgta caaaaaagca
     1801 ggctttaaag gaaccaattc agtcgactgg atcccgagtt tactccctat cagtgataga
     1861 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttantccct
     1921 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     1981 aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     2041 cgccactaga tggactacaa agaccatgac ggtgattata aagatcatga catcgattac
     2101 aaggatgacg atgacaagac tagaatgtct cgggagtcgg atgttgaggc tcagcagtct
     2161 catggcagca gtgcctgttc acagccccat ggcagcgtta cccagtccca aggctcctcc
     2221 tcacagtccc agggcatatc cagctcctct accagcacga tgccaaactc cagccagtcc
     2281 tctcactcca gctctgggac actgagctcc ttagagacag tgtccactca ggaactctat
     2341 tctattcctg aggaccaaga acctgaggac caagaacctg aggagcctac ccctgccccc
     2401 tgggctcgat tatgggccct tcaggatgga tttgccaatc ttgaatgtgt gaatgacaac
     2461 tactggtttg ggagggacaa aagctgtgaa tattgctttg atgaaccact gctgaaaaga
     2521 acagataaat accgaacata cagcaagaaa cactttcgga ttttcaggga agtgggtcct
     2581 aaaaactctt acattgcata catagaagat cacagtggca atggaacctt tgtaaataca
     2641 gagcttgtag ggaaaggaaa acgccgtcct ttgaataaca attctgaaat tgcactgtca
     2701 ctaagcagaa ataaagtttt tgtctttttt gatctgactg tagatgatca gtcagtttat
     2761 cctaaggcat taagagatga atacatcatg tcaaaaactc ttggaagtgg tgcctgtgga
     2821 gaggtaaagc tggctttcga gaggaaaaca tgtaagaaag tagccataaa gatcatcagc
     2881 aaaaggaagt ttgctattgg ttcagcaaga gaggcagacc cagctctcaa tgttgaaaca
     2941 gaaatagaaa ttttgaaaaa gctaaatcat ccttgcatca tcaagattaa aaactttttt
     3001 gatgcagaag attattatat tgttttggaa ttgatggaag ggggagagct gtttgacaaa
     3061 gtggtgggga ataaacgcct gaaagaagct acctgcaagc tctattttta ccagatgctc
     3121 ttggctgtgc agtaccttca tgaaaacggt attatacacc gtgacttaaa gccagagaat
     3181 gttttactgt catctcaaga agaggactgt cttataaaga ttactgattt tgggcactcc
     3241 aagattttgg gagagacctc tctcatgaga accttatgtg gaacccccac ctacttggcg
     3301 cctgaagttc ttgtttctgt tgggactgct gggtataacc gtgctgtgga ctgctggagt
     3361 ttaggagtta ttctttttat ctgccttagt gggtatccac ctttctctga gcataggact
     3421 caagtgtcac tgaaggatca gatcaccagt ggaaaataca acttcattcc tgaagtctgg
     3481 gcagaagtct cagagaaagc tctggacctt gtcaagaagt tgttggtagt ggatccaaag
     3541 gcacgtttta cgacagaaga agccttaaga cacccgtggc ttcaggatga agacatgaag
     3601 agaaagtttc aagatcttct gtctgaggaa aatgaatcca cagctctacc ccaggttcta
     3661 gcccagcctt ctactagtcg aaagcggccc cgtgaagggg aagccgaggg tgccgagacc
     3721 acaaagcgcc cagctgtgtg tgctgctgtg ttgtgacaat tggaagacta atgcgttttc
     3781 gagatatcta gacccagctt tcttgtacaa agtggttgat aacatctcga gccataaaga
     3841 tggttaatta acccacccaa gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc
     3901 gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat
     3961 ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc
     4021 cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc
     4081 tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga
     4141 tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta
     4201 gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac
     4261 ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg
     4321 ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc
     4381 cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg
     4441 aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg
     4501 caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg
     4561 ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt
     4621 tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt
     4681 tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg
     4741 gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc
     4801 gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata
     4861 agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct
     4921 ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc
     4981 ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc
     5041 cgctaaattc tggccgtttt tggctttttt gttagacgaa gcttggtacc gagctcggat
     5101 ctccaccccg taccggtcct gcagtcgaat tcaccatgtc tagactggac aagagcaaag
     5161 tcataaacgg agctctggaa ttactcaatg gtgtcggtat cgaaggcctg acgacaagga
     5221 aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc
     5281 gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc
     5341 ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg
     5401 ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac
     5461 agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga
     5521 acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac
     5581 aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac
     5641 ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg
     5701 gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga
     5761 ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg
     5821 accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt
     5881 aactaagtaa ggatccgcgg ccgcactaga ggaattccgc ccctctcccc ccccccctmw
     5941 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     6001 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     6061 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     6121 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     6181 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     6241 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     6301 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     6361 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     6421 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     6481 acgatgataa gcttaccggt ccaccatgat tgaacaagat ggattgcacg caggttctcc
     6541 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     6601 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     6661 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     6721 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     6781 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     6841 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     6901 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6961 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     7021 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     7081 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     7141 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     7201 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     7261 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga tgtacaagta aagcggccgc
     7321 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     7381 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gtttagtccc
     7441 tcccaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     7501 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7561 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7621 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7681 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7741 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7801 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7861 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7921 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7981 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8041 tgggccgcct ccccgcatcg ataccgtcga cctcgatcga gacctagaaa aacatggagc
     8101 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     8161 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     8221 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     8281 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     8341 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     8401 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     8461 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     8521 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     8581 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8641 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8701 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8761 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     8821 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     8881 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     8941 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     9001 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     9061 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     9121 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     9181 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     9241 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     9301 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     9361 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     9421 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     9481 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     9541 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     9601 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9661 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     9721 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     9781 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     9841 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     9901 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     9961 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
    10021 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
    10081 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
    10141 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
    10201 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
    10261 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
    10321 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
    10381 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
    10441 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    10501 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10561 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
    10621 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    10681 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    10741 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    10801 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    10861 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    10921 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    10981 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    11041 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    11101 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    11161 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    11221 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    11281 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    11341 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    11401 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    11461 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    11521 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    11581 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    11641 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    11701 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    11761 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    11821 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    11881 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    11941 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    12001 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    12061 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    12121 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    12181 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    12241 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    12301 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    12361 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    12421 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    12481 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    12541 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    12601 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    12661 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    12721 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    12781 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
    12841 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
    12901 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
    12961 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
    13021 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
    13081 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
    13141 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
    13201 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
    13261 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
    13321 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
    13381 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
    13441 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
    13501 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
    13561 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tact
//
