LOCUS       sequence-291742-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express SARS-CoV spike protein with C9 tag at C-terminal in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Shang J, Ye G, Shi K, Wan Y, Luo C, Aihara H, Geng Q, Auerbach A, Li
            F
  TITLE     Structural basis of receptor recognition by SARS-CoV-2.
  JOURNAL   Nature. 2020 Mar 30. pii: 10.1038/s41586-020-2179-y. doi: 
            10.1038/s41586-020-2179-y.
  PUBMED    32225175
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2020 Mar 30. pii: 10.1038/s41586-020-2179-y. doi: 
            10.1038/s41586-020-2179-y."
COMMENT     Sequence Label: pcDNA3.1-SARS-Spike
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    260..381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(297..316)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     351..370
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(430..446)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(430..446)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(443..465)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    454..470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(478..484,485..502,503..508))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    523..544
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(661..678)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(908..927)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgaccgctt cctcgtgctt tanggtatcg ccgctcccga ttcgcagcgc atcgccttct
       61 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
      121 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
      181 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
      241 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
      301 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
      361 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
      421 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      481 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      541 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      601 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      661 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      721 naaggcggta atacggttat ccacagaatc nggggataac gcnnaaagaa catgtgagca
      781 aaannncagc aaaaggncan gaaccgtaaa aanncgcgtt gctggcgttt ttccatnnct
      841 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt canagtggcg aaacccgaca
      901 ggactataaa gataccaggc gtttccccct gnagctccct cgtgcgctct ctgttccgac
      961 cctgccgctt accngatacc tgtcgctt
//
