LOCUS       sequence-291746-        4381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For making myc-T58A-S62D RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Williams C
  TITLE     Williams Lab Plasmids 2020
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIVT-myc-T58A-S62D
FEATURES             Location/Qualifiers
     source          1..4381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           62..104
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     3'UTR           1531..1656
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(1712..1729)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1712..1728)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1721..1743)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1937..1956)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2056..2078
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2116..2134)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2202..2306
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2307..2375,2376..3167)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2525..2544)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3338..3926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3827..3846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4080..4097
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4214..4235
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4250..4255,4256..4273,4274..4280)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4288..4304
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4293..4315
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4312..4328
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4312..4328
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4352..4370
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4352..4369
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 taatacgact cactataggg agagagagag aattaccctc actaaaggga ggagaagctt
       61 gcttgttctt tttgcagaag ctcagaataa acgctcaact ttggcagatc aagcttgcgt
      121 gcctgcaggt cgacgccacc atgcccctca acgtgaactt caccaacagg aactatgacc
      181 tcgactacga ctccgtacag ccctatttca tctgcgacga ggaagagaat ttctatcacc
      241 agcaacagca gagcgagctg cagccgcccg cgcccagtga ggatatctgg aagaaattcg
      301 agctgcttcc cgccccgccc ctggacccga gccgccgctc cgggctctgc tctccatcct
      361 atgttgcggt cgctacgtcc ttctccccaa gggaagacga tgacggcggc ggtggcaact
      421 tctccaccgc cgatcagctg gagatgatga ccgagttact tggaggagac atggtgaacc
      481 agagcttcat ctgcgatcct gacgacgaga ccttcatcaa gaacatcatc atccaggact
      541 gtatgtggag cggtttctca gccgctgcca agctggtctc ggagaagctg gcctcctacc
      601 aggctgcgcg caaagacagc accagcctga gccccgcccg cgggcacagc gtctgctcca
      661 cctccagcct gtacctgcag gacctcaccg ccgccgcgtc cgagtgcatt gacccctcag
      721 tggtctttcc ctacccgctc aacgacagca gctcgcccaa atcctgtacc tcgtccgatt
      781 ccacggcctt ctctccttcc tcggactcgc tgctgtcctc cgagtcctcc ccacgggcca
      841 gccctgagcc cctagtgctg catgaggaga caccgcccac caccagcagc gactctgaag
      901 aagagcaaga agatgaggaa gaaattgatg tggtgtctgt ggagaagagg caaacccctg
      961 ccaagaggtc ggagtcgggc tcatctccat cccgaggcca cagcaaacct ccgcacagcc
     1021 cactggtcct caagaggtgc cacgtctcca ctcaccagca caactacgcc gcacccccct
     1081 ccacaaggaa ggactatcca gctgccaaga gggccaagtt ggacagtggc agggtcctga
     1141 agcagatcag caacaaccgc aagtgctcca gccccaggtc ctcagacacg gaggaaaacg
     1201 acaagaggcg gacacacaac gtcttggaac gtcagaggag gaacgagctg aagcgcagct
     1261 tttttgccct gcgtgaccag atccctgaat tggaaaacaa cgaaaaggcc cccaaggtag
     1321 tgatcctcaa aaaagccacc gcctacatcc tgtccattca agcagacgag cacaagctca
     1381 cctctgaaaa ggacttattg aggaaacgac gagaacagtt gaaacacaaa ctcgaacagc
     1441 ttcgaaactc tggtgcataa tctagaggat ccccgggtac cgagctcgaa ttcgcccttc
     1501 tagtgactga ctaggatctg gttaccacta aaccagcctc aagaacaccc gaatggagtc
     1561 tctaagctac ataataccaa cttacacttt acaaaatgtt gtcccccaaa atgtagccat
     1621 tcgtatctgc tcctaataaa aagaaagttt cttcacattc taaaaaaaaa aaaaaaaaaa
     1681 aaaaaaamcc ccccccccca agggcgaatt cactggccgt cgttttacaa cgtcgtgact
     1741 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     1801 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     1861 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     1921 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     1981 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     2041 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     2101 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     2161 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2221 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2281 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2341 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2401 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2461 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2521 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2581 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2641 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2701 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2761 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2821 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     2881 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2941 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3001 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3061 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3121 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3181 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3241 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3361 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3421 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3481 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3541 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3601 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3661 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3721 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3781 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3841 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3901 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3961 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4021 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4081 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4141 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4201 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     4261 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     4321 gctatgacca tgattacgcc aagctccaac gatttaggtg acactataga agagaaggaa
     4381 t
//
