LOCUS       sequence-291757-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET vector for IPTG-inducible, T7-based expression of the 
            exonuclease deficient version of RTX bearing a C-terminal 6xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Bhadra S, Maranhao AC, Paik I, Ellington AD
  TITLE     A one-enzyme RT-qPCR assay for SARS-CoV-2, and procedures for 
            reagent production
  JOURNAL   BioProtocol 2021
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BioProtocol
            2021"
COMMENT     Sequence Label: pET_RTX(exo-)-6xHis
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             451..459
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gttagcagcc ggatcaatgc ggccgcttat cagtgatggt gatggtgatg gctggatccc
       61 tcgagggtac ctttcggctt cagacgggca ctcaaaccga cttgacgagt tttctggtac
      121 cgcaaatcct ccttgcgata gccgaacgca cgtaagatgc gctccaccgc cggcagaacc
      181 tgtttttcaa tgtaatattc cgcatcatat ttatgcttcg tcggatcaaa ttcatcaaat
      241 ggaatcgcgc gatcaacaat ccggcctgag cctttgagga cgatgtaact aataaccgtg
      301 cctggccgaa tcttgacgcc ccgagccgca agccgctttg ctacggcgac gtgtggacca
      361 gtggccttat agtctttaag gtcgcgagtg atttgtttat gaataacaag tttctctggt
      421 gggacctcat acttgctaag cttctctgta acctccttaa cgatgcgtac ggctttttcg
      481 acgtcaccat ccttcaggag ggcctccaac acacgagctt gggtctcctt tgcgatctcg
      541 ctccaatcgc gacgaacaat ttccaagcca cgagttgtga tcttgccttc ctcgtcaatt
      601 accgcgtatt tcttctttgt cacgaacagg ccacgcttat aaaaaccttc gtactctaat
      661 tccagggcgc ctggcaactt cgcattaata tacttcaaga actccattgc ctttttcttt
      721 acagtttctg cgtccgcacc tggaatcgtt gcaaagaaac catccgtgtc actatagatg
      781 accttgaaac cgtatttctc ctcaatttcc ttaatcgtca tggtcaggta ctcccgaccc
      841 caagcaatga ctgattctgc acattctttg caataccacc gggcgcgtgc gtagccgtaa
      901 taaccgtaca gagagttcgc gaggatcttg attgcacgct gacggtaatc aagtaatttg
      961 cgttcaattg ggtcaattgt cgctttcata cgttt
//
