LOCUS       sequence-291767-       10614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Light-induced recruitment of NSImb-QPAS1-mCherry to the target 
            protein bound to membrane; interaction occurs via intrabody fused to
            BphP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Redchuk TA, Karasev MM, Verkhusha PV, Donnelly SK, Hulsemann M, 
            Virtanen J, Moore HM, Vartiainen MK, Hodgson L, Verkhusha VV
  TITLE     Optogenetic regulation of endogenous proteins.
  JOURNAL   Nat Commun. 2020 Jan 30;11(1):605. doi: 10.1038/s41467-020-14460-4.
  PUBMED    32001718
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-30"; volume: "11(1):605. doi"; pages: " 
            10.1038/s41467-020-14460-4"
COMMENT     Sequence Label: pQP-2376
FEATURES             Location/Qualifiers
     source          1..10614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        110..413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        414..617
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     568..588
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(652..654,655..657,658..1368)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     ERVTAAGITLGMDELYK"
     primer_bind     complement(697..718)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(958..977)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    1423..1479
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    1481..2054
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1504..2056
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     regulatory      2087..2096
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4354..4695
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
     misc_feature    4705..4761
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    4763..5337
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4787..5339
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6011..6718
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6156..6174)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6424..6443
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7223..7237
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     polyA_signal    7451..7572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7488..7507)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7542..7561
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7579..8034)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7716..7737)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7928..7947
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8061..8165
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8167..8524
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8167..8358
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8190..8210)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8375..8510
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8559..9353
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8559..9353
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8613..8632)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9223..9242
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9541..9560)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9585..9632
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9961..10549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10450..10469
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat
       61 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      121 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      181 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      241 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      301 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      361 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      421 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      481 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      541 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      601 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac catggtgagc
      661 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      721 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      781 accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      841 accctgggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
      901 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      961 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1021 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1081 tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
     1141 gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac
     1201 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctaagc
     1261 taccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggaa
     1321 cgcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggc tagcggtaaa
     1381 aagaagaaaa agaagtcaaa gacaaagtgt gtaattatgt aaagatctcg agctcaagct
     1441 tcgaattctg cagtcgacgg taccgcgggc ccgggatccg cccctctccc tccccccccc
     1501 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     1561 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     1621 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     1681 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     1741 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     1801 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     1861 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     1921 aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt
     1981 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     2041 aaacacgatg ataatatggc cacaagcgct accggactca gatctaccat ggtggcaggt
     2101 catgcctctg gcagccccgc attcgggacc gccgatcttt cgaattgcga acgtgaagag
     2161 atccacctcg ccggctcgat ccagccgcat ggcgcgcttc tggtcgtcag cgagccggat
     2221 catcgcatca tccaggccag cgccaacgcc gcggaatttc tgaatctcgg aagcgtgctc
     2281 ggcgttccgc tcgccgagat cgacggcgat ctgttgatca agatcctgcc gcatctcgat
     2341 cccaccgccg aaggcatgcc ggtcgcggtg cgctgccgga tcggcaatcc ctccacggag
     2401 tacgacggtc tgatgcatcg gcctccggaa ggcgggctga tcatcgagct cgaacgtgcc
     2461 ggcccgccga tcgatctgtc cggcacgctg gcgccggcgc tggagcggat ccgcacggcg
     2521 ggctcgctgc gcgcgctgtg cgatgacacc gcgctgctgt ttcagcagtg caccggctac
     2581 gaccgggtga tggtgtatcg cttcgacgag cagggccacg gcgaagtgtt ctccgagcgc
     2641 cacgtgcccg ggctcgaatc ctatttcggc aaccgctatc cgtcgtcgga cattccgcag
     2701 atggcgcggc ggctgtacga gcggcagcgc gtccgcgtgc tggtcgacgt cagctatcag
     2761 ccggtgccgc tggagccgcg gctgtcgccg ctgaccgggc gcgatctcga catgtcgggc
     2821 tgcttcctgc gctcgatgtc gccgatccat ctgcagtacc tgaagaacat gggcgtgcgc
     2881 gccaccctgg tggtgtcgct ggtggtcggc ggcaagctgt ggggcctggt tgcctgtcac
     2941 cattatctgc cgcgcttcat ccatttcgag ctgcgggcga tctgcgaact gctcgccgaa
     3001 gcgatcgcga cgcggatcac cgcgcttgag agcttcgcgc agagccagtc ggagctgttc
     3061 gtgcagcggc tcgaacagcg catgatcgaa gcgatcaccc gtgaaggcga ttggcgcgca
     3121 gcgattttcg acaccagcca atcgatcctg cagccgctgc acgccgacgg ttgcgcgctg
     3181 gtgtacgaag accagatcag gaccatcggt gacgtacctt ccacgcagga tgttcgcgag
     3241 atcgccgggt ggctcgatcg ccagccacgt gcggcggtga cctcgaccgc gtcgctcggt
     3301 ctcgacgtgc cggagctcgc gcatctgacg cggatggcga gcggcgtggt cgcggcgccg
     3361 atttcggatc atcgcggcga gtttctgatg tggttccgcc ccgagcgcgt ccacaccgtt
     3421 acctggggcg gcgatccgaa gaagccgttc acgatgggcg atacaccggc ggatctgtcg
     3481 ccgcggcgct ccttcgccaa atggcatcag gttgtcgaag gcacgtccga tccgtggacg
     3541 gccgccgatc tcgccgcggc tcgcaccatc ggtcagaccg tcgccgacat cgtgctgcaa
     3601 ttccgcgcgg tgcggacact gatcgcccgc gaacagtacg aacagttttc gtcccaggtg
     3661 cacgcttcga tgcagccggt gctgatcacc gacgccgaag gccgcatcct gctgatgaac
     3721 gactcgttcc gcgacatgtt gccggcgggg tcgccatccg ccgtccatct cgacgatctc
     3781 gccgggttct tcgtcgaatc gaacgatttc ctgcgcaacg tcgccgaact gatcgatcac
     3841 ggccgcgggt ggcgcggcga agttctgctg cgcggcgcag gtaatcgccc gttgccgctg
     3901 gcagtgcgcg ccgatccggt gacgcgcacg gaggaccagt cgctcggctt cgtgctgatc
     3961 ttcagcgacg ctaccgatcg tcgcaccgca gatgccgcac gcacgcgttt ccaggaaggc
     4021 attcttgcca gcgcacgtcc cggcgtgcgg ctcgactcca agtccgacct cttgcacgag
     4081 aagctgctgt ccgcgctggt cgagaacgcg cagcttgccg cattggaaat tacttacggc
     4141 gtcgagaccg gacgcatcgc cgagctgctc gaaggcgttc gccagtcgat gctgcgcacc
     4201 gccgaagtgc tcggccatct ggtgcagcac gcggcgcgca cggccggcag cgacagctcg
     4261 agcaatggct cgcagaacaa gaaggctagc agtgctggcg gtagtgctgg tgggagtgct
     4321 ggcggtagtg ctggtggcga tatcatggat caagtccaac tggtggagtc tggtggcgct
     4381 ttggtgcagc caggtggctc tctgcgtttg tcctgtgccg cttctggctt cccagtgaac
     4441 cgctattcca tgcgctggta tcgccaggct ccaggcaaag agcgtgagtg ggtagccggt
     4501 atgtccagcg cgggtgatcg tagctcctat gaagactccg tgaagggccg tttcaccatc
     4561 agccgtgacg atgcccgtaa cacggtgtat ctgcaaatga acagcttgaa acctgaagat
     4621 acggccgtgt attactgtaa tgtgaacgtg ggcttcgagt attggggcca aggcacccag
     4681 gtcaccgtct ccagctaaga tatcagatct cgagctcaag cttcgaattc tgcagtcgac
     4741 ggtaccgcgg gcccgggatc cgcccctctc cctccccccc cccctaacgt tactggccga
     4801 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     4861 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     4921 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     4981 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     5041 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     5101 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     5161 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     5221 ggatctgatc tggggcctcg gtacacatgc tttacatgtg tttagtcgag gttaaaaaaa
     5281 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat
     5341 ggccacaaac cggtccatga tgaaaatgga gactgacaaa ataatggacg aaaccaactc
     5401 caatgcacag gccttcacaa ccactatgct gtacgacccg gtgcgcaaga aagactcatc
     5461 gcccacctac caaacggagc gggaactctg ctttcagtac ttcacccagt ggagcgagtc
     5521 gggccaggtg gactttgtgg agcacctgct gtcgcgcatg tgccactatc aacacggaca
     5581 gatcaatgcc tatctcaagc cgatgctcca gcgggacttt atcacattgc tgccaatcaa
     5641 gggtctggat cacatcgcag aaaacatttt gtcgtacttg gatgccgaat cgctcaaatc
     5701 atccgagctg gtctgcaagg aatggctgcg cgtcatttcc gagggcatgc tctggaagaa
     5761 gctcatcgaa cgcaaggtgc gcacagattc cttgtggcgc ggactggccg agcggcgtaa
     5821 ttggatgcag tacctcttca agccaagacc gggccagact caacggccac actcattcca
     5881 tcgcgagttg ttccccaaga taatgaatga cattgacagc atagagaaca actggcggac
     5941 tggccgccac ggaggcggat cataccggtc catgggaatt gatctctcag gattaacact
     6001 gcagactagt atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     6061 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     6121 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     6181 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     6241 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     6301 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     6361 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     6421 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     6481 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     6541 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     6601 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     6661 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagag
     6721 tgctggtggg tccgccggcg ggagtgctgg cggtagtgcc ggcggtagtt ctagaggcaa
     6781 gaacatgcag gcggtcaccg agctgcattc ccggctgatc gctgcgcagc aggcgatgga
     6841 gcgcgactat tggcggttgc gtgaattgga gactcgctac cgcctggtgt tcgacgctgc
     6901 cgccgatgcg gtgatgatcg tctccgccgg cgacatgcgc atcgtcgaag ccaaccgggc
     6961 ggcggtgaat gcgatcagcc gcgtcgagcg cggcaatgac gaccttgcgg ggcgtgattt
     7021 cctcgccgaa gtggcggctg ccgatcgcga tgcggtgcgc gacatgctgg cccaggtgcg
     7081 tcagcgcggc accgcactca gcgtcctcgt tcatctcggc cgttacgacc gcgcctggat
     7141 gctgcgcggt tcgctgatgt cgtccgagcg tcgtcaggtt ttcctgctgc acttcacccc
     7201 ggtgaccacg actcccgcga tcgacgacga cgacaaaggt gtcgttgctt ctgctgccga
     7261 cggtgcagag ggcgcctcag acgacgcaga ggatgaatta tggctgccaa gagggtcaag
     7321 ttggactgag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
     7381 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     7441 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7501 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7561 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     7621 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     7681 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     7741 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     7801 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     7861 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     7921 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     7981 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     8041 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8101 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8161 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     8221 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8281 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8341 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8401 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8461 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8521 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     8581 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     8641 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     8701 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     8761 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     8821 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     8881 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     8941 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     9001 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     9061 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     9121 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     9181 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     9241 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     9301 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     9361 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     9421 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     9481 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     9541 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     9601 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     9661 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     9721 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     9781 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     9841 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     9901 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9961 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    10021 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    10081 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    10141 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    10201 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    10261 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    10321 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    10381 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    10441 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    10501 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    10561 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctt
//
