LOCUS       sequence-291774-       10728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3xMYC-SIX2 expression. Vector also expresses GFP under control of 
            hUBC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10728)
  TITLE     Kingsbury Lab Plasmids
REFERENCE   2  (bases 1 to 10728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTJK477
FEATURES             Location/Qualifiers
     source          1..10728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    44..65
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(80..85,86..103,104..110)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    118..134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     123..145
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     142..158
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     142..158
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     177..197
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        179..197
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        298..677
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        678..881
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     832..852
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             896..1076
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1123..1248
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1741..1974
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2159..2203
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2496..2613
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2665..3843
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2896..3834
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3791..3811
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3848..3857
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3857..3886
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3890..3919
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3923..3952
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        4852..6063
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5900..5919
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6070..6072,6073..6075,6076..6789)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6070..6072,6073..6075,6076..6786)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6115..6136)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6376..6395)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6723..6744
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6868..6888)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7286..7297)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7471..7704
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7776..7897
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7813..7832)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7867..7886
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7937..8072
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8092..8111)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8093..8111)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8121..8138)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8121..8137)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8130..8152)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8279..8734
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8366..8385)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8576..8597
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8760..8864
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8865..8933,8934..9725)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9083..9102)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9896..10484
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10385..10404
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10638..10655
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
       61 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      121 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
      181 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttggccattg catacgttgt
      241 atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac
      301 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      361 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      421 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      481 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      541 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      601 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      661 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      721 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      781 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      841 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccggggtc
      901 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      961 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     1021 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     1081 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     1141 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     1201 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     1261 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     1321 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1381 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1441 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     1501 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     1561 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     1621 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1681 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1741 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1801 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1861 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1921 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1981 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     2041 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     2101 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     2161 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     2221 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     2281 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     2341 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2401 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     2461 tcgatctcga cacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     2521 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2581 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     2641 ccagtttggg tcgaggattc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg
     2701 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     2761 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     2821 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     2881 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
     2941 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
     3001 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
     3061 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
     3121 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     3181 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
     3241 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     3301 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     3361 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     3421 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     3481 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     3541 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     3601 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt
     3661 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     3721 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     3781 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     3841 tgagctagcc accatggaac aaaaactcat ctcagaagag gatctgattg aacaaaagct
     3901 catctcagag gaagatctga tggagcagaa actgatcagc gaagaggacc tcatgtccat
     3961 gctgcccacc ttcggcttca cgcaggagca agtggcgtgc gtgtgcgagg tgctgcagca
     4021 gggcggcaac atcgagcggc tgggccgctt cctgtggtcg ctgcccgcct gcgagcacct
     4081 tcacaagaat gaaagcgtgc tcaaggccaa ggccgtggtg gccttccacc gcggcaactt
     4141 ccgcgagctc tacaagatcc tggagagcca ccagttctcg ccgcacaacc acgccaagct
     4201 gcagcagctg tggctcaagg cacactacat cgaggcggag aagctgcgcg gccgacccct
     4261 gggcgccgtg ggcaaatacc gcgtgcgccg caaattcccg ctgccgcgct ccatctggga
     4321 cggcgaggag accagctact gcttcaagga aaagagtcgc agcgtgctgc gcgagtggta
     4381 cgcgcacaac ccctaccctt caccccgcga gaagcgtgag ctggcggagg ccacgggcct
     4441 caccaccaca caggtcagca actggttcaa gaaccggcgg cagcgcgacc gggcggccga
     4501 ggccaaggaa agggagaaca acgagaactc caattctaac agccacaacc cgctgaatgg
     4561 cagcggcaag tcggtgttag gcagctcgga ggatgagaag actccatcgg ggacgccaga
     4621 ccactcatca tccagccccg cactgctcct cagcccgccg ccccctgggc tgccgtccct
     4681 gcacagcctg ggccaccctc cgggccccag cgcagtgcca gtgccggtgc caggcggagg
     4741 tggagcggac ccactgcaac accaccatgg cctgcaggac tccatcctca accccatgtc
     4801 agccaacctc gtggacctgg gctcctaggt cgacggatcc cccgggctgc aggaattcgc
     4861 gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag
     4921 acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct
     4981 gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact
     5041 tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt
     5101 cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata
     5161 aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct
     5221 tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc
     5281 tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg
     5341 tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat
     5401 ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga
     5461 aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc
     5521 gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag
     5581 tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt
     5641 gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca
     5701 cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct
     5761 tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg
     5821 ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt
     5881 acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt
     5941 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
     6001 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
     6061 gacctcgaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     6121 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     6181 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     6241 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     6301 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     6361 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     6421 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     6481 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     6541 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     6601 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     6661 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     6721 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     6781 tacaagtaag gatatcggta ccgcatgcgt ttaaactcaa cctctggatt acaaaatttg
     6841 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6901 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6961 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7021 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7081 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7141 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7201 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     7261 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7321 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7381 ctccctttgg gccgcctccc cgcactttaa gaccaatgac ttacaaggca gctgtagatc
     7441 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7501 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7561 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7621 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7681 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     7741 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     7801 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7861 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     7921 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7981 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8041 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     8101 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     8161 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     8221 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     8281 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8341 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8401 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8461 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     8521 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8581 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     8641 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     8701 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     8761 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     8821 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8881 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8941 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     9001 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     9061 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     9121 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     9181 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     9241 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     9301 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     9361 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     9421 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     9481 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     9541 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     9601 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     9661 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     9721 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     9781 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9841 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     9901 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     9961 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    10021 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    10081 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    10141 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    10201 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    10261 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    10321 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    10381 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    10441 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    10501 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    10561 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    10621 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    10681 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacg
//
