LOCUS       sequence-291775-       11631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BirA*-SIX2 expression. Vector also expresses GFP under control of 
            hUBC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11631)
  TITLE     Kingsbury Lab Plasmids
REFERENCE   2  (bases 1 to 11631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTJK421
FEATURES             Location/Qualifiers
     source          1..11631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    82..103
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(118..123,124..141,142..148)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    156..172
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     161..183
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     180..196
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     180..196
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     215..235
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        217..235
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        336..715
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        716..919
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     870..890
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             934..1114
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1161..1286
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1779..2012
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2197..2241
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2534..2651
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2703..3881
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2934..3872
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3829..3849
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3886..3895
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3895..3924
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(3928..4275,4276..4278,4279..4887)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     promoter        5793..7004
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6841..6860
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(7011..7013,7014..7016,7017..7730)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7011..7013,7014..7016,7017..7727)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7056..7077)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7317..7336)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7664..7685
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7809..7829)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8227..8238)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8412..8645
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8717..8838
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8754..8773)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8808..8827
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8878..9013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9033..9052)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9034..9052)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9062..9079)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9062..9078)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9071..9093)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9220..9675
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9307..9326)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9517..9538
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9701..9805
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9806..9874,9875..10666)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10024..10043)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10837..11425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11326..11345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11579..11596
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
       61 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
      121 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      181 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
      241 aaagctggag ctgcaagctt ggccattgca tacgttgtat ccatatcata atatgtacat
      301 ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta
      361 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      421 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      481 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      541 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      601 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      661 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      721 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      781 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      841 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      901 ggtctatata agcagagctc gtttagtgaa ccggggtctc tctggttaga ccagatctga
      961 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     1021 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     1081 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     1141 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     1201 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     1261 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     1321 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     1381 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1441 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1501 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1561 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1621 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1681 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1741 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1801 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1861 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1921 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1981 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     2041 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     2101 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     2161 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     2221 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     2281 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     2341 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     2401 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     2461 gacagatcca ttcgattagt gaacggatct cgacggtatc gatctcgaca caaatggcag
     2521 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2581 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2641 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttgggtc gaggattcgt
     2701 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2761 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2821 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2881 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2941 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     3001 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     3061 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     3121 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     3181 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     3241 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     3301 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     3361 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     3421 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3481 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3541 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3601 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3661 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3721 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3781 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3841 cagtggttca aagttttttt cttccatttc aggtgtcgtg agctagccac catggaacaa
     3901 aaactcatct cagaagagga tctcgacaag gacaacaccg tgcccctgaa gctgatcgcc
     3961 ctgctggcca acggcgagtt ccactctggc gagcagctgg gagagaccct gggaatgagc
     4021 agagccgcca tcaacaagca catccagaca ctgagagact ggggagtgga cgtgttcacc
     4081 gtgcctggca agggctacag cctgcctgag cctatccagc tgctgaacgc caagcagatc
     4141 ctgggacagc tggatggcgg aagcgtggcc gtgctgcctg tgatcgactc caccaatcag
     4201 tacctgctgg acagaatcgg agagctgaag tccggcgacg cctgcatcgc cgagtaccag
     4261 caggctggca gaggaggcag aggacggaag tggttcagcc cattcggagc caacctgtac
     4321 ctgtccatgt tctggagact ggagcaggga cctgctgctg ccatcggact gagtctggtg
     4381 atcggaatcg tgatggccga ggtgctgaga aagctgggag ccgacaaggt gagagtgaag
     4441 tggcctaatg acctgtacct ccaggaccgc aagctggctg gcatcctggt ggagctgaca
     4501 ggcaagacag gcgatgccgc tcagatcgtg atcggagccg gaatcaacat ggccatgaga
     4561 agagtggagg agagcgtggt gaaccagggc tggatcaccc tgcaggaggc tggcatcaac
     4621 ctggaccgga acaccctggc cgccatgctg atcagagagc tgagagccgc tctggagctg
     4681 ttcgagcagg agggactggc tccttacctg agcagatggg agaagctgga caacttcatc
     4741 aacagacctg tgaagctgat catcggcgac aaggaaatct tcggcatctc cagaggaatc
     4801 gacaagcagg gagctctgct gctggagcag gacggaatca tcaagccctg gatgggcgga
     4861 gaaatctccc tgagaagcgc agagaagtct agaatgtcca tgctgcccac cttcggcttc
     4921 acgcaggagc aagtggcgtg cgtgtgcgag gtgctgcagc agggcggcaa catcgagcgg
     4981 ctgggccgct tcctgtggtc gctgcccgcc tgcgagcacc ttcacaagaa tgaaagcgtg
     5041 ctcaaggcca aggccgtggt ggccttccac cgcggcaact tccgcgagct ctacaagatc
     5101 ctggagagcc accagttctc gccgcacaac cacgccaagc tgcagcagct gtggctcaag
     5161 gcacactaca tcgaggcgga gaagctgcgc ggccgacccc tgggcgccgt gggcaaatac
     5221 cgcgtgcgcc gcaaattccc gctgccgcgc tccatctggg acggcgagga gaccagctac
     5281 tgcttcaagg aaaagagtcg cagcgtgctg cgcgagtggt acgcgcacaa cccctaccct
     5341 tcaccccgcg agaagcgtga gctggcggag gccacgggcc tcaccaccac acaggtcagc
     5401 aactggttca agaaccggcg gcagcgcgac cgggcggccg aggccaagga aagggagaac
     5461 aacgagaact ccaattctaa cagccacaac ccgctgaatg gcagcggcaa gtcggtgtta
     5521 ggcagctcgg aggatgagaa gactccatcg gggacgccag accactcatc atccagcccc
     5581 gcactgctcc tcagcccgcc gccccctggg ctgccgtccc tgcacagcct gggccaccct
     5641 ccgggcccca gcgcagtgcc agtgccggtg ccaggcggag gtggagcgga cccactgcaa
     5701 caccaccatg gcctgcagga ctccatcctc aaccccatgt cagccaacct cgtggacctg
     5761 ggctcctagg tcgacggatc ccccgggctg caggaattcg cgccgggttt tggcgcctcc
     5821 cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg
     5881 tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt
     5941 agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact
     6001 ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc
     6061 ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc
     6121 acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga
     6181 tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg
     6241 ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca
     6301 aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct
     6361 tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg
     6421 tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg
     6481 tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa
     6541 ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc
     6601 gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata
     6661 atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc
     6721 agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg
     6781 agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc
     6841 tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt
     6901 aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta
     6961 aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacctcgag atggtgagca
     7021 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     7081 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     7141 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     7201 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     7261 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     7321 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     7381 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     7441 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     7501 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     7561 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     7621 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     7681 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa ggatatcggt
     7741 accgcatgcg tttaaactca acctctggat tacaaaattt gtgaaagatt gactggtatt
     7801 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     7861 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7921 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     7981 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     8041 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     8101 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     8161 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     8221 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     8281 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     8341 ccgcacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     8401 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     8461 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8521 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8581 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8641 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     8701 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8761 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8821 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     8881 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8941 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9001 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     9061 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     9121 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     9181 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     9241 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     9301 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     9361 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     9421 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     9481 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     9541 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     9601 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     9661 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     9721 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     9781 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     9841 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     9901 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     9961 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
    10021 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
    10081 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
    10141 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
    10201 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
    10261 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    10321 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    10381 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    10441 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    10501 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    10561 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    10621 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    10681 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    10741 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    10801 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10861 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10921 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10981 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    11041 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    11101 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    11161 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    11221 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    11281 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    11341 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    11401 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    11461 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    11521 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    11581 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc t
//
