LOCUS       sequence-291783-        4821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR delivery and repair template delivery vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   de Maat V, Stege PB, Dedden M, Hamer M, van Pijkeren JP, Willems 
            RJL, van Schaik W
  TITLE     CRISPR-Cas9-mediated genome editing in vancomycin-resistant 
            Enterococcus faecium.
  JOURNAL   FEMS Microbiol Lett. 2019 Nov 1;366(22). pii: 5697197. doi: 
            10.1093/femsle/fnz256.
  PUBMED    31905238
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol Lett."; date: "2019-11-1"; volume: "366(22). pii: 5697197.
            doi"; pages: " 10.1093/femsle/fnz256"
COMMENT     Sequence Label: pVDM1001
FEATURES             Location/Qualifiers
     source          1..4821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(14..30)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14..30)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(2072..2083)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4086..4108
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4100..4117
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4101..4117
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4127..4146
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4127..4145
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   4292..4327
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   4358..4393
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(4394..4525)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
ORIGIN
        1 ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtggg atcctctaga
       61 gtctagggac ctctttagct ccttggaagc tgtcagtagt agggacgctt tattcttccc
      121 aaaaattgtt caatatttat caataaatca gtagttttaa aagtaagcac ctgttattgc
      181 aataaaatta gcctaattga gagaagtttc tatagaattt ttcatatact taacgagtgc
      241 tttcaccttt gaatatagtc cttcccactt atcatcacac tctccccgat agccttttct
      301 agctatatcc agtaaagtta catgctcttt aggtaaaaga ggtatagccc attctgcagc
      361 gacatctttc gaggtaattt caccagtagt cactgtttgc cacattcgag ctagggttaa
      421 aattacatta cgctcatcac cttttatccc ctcaattagt tctggcaaag aatccttaat
      481 tgctcttcga atatctgtca aaggtacgga gacaagtata cttgaagaat caggaccaaa
      541 tagagaaata ctattctttc ttgcttgtgc taaaacaata gccaaatcag gatcatagct
      601 tggttcctga atttgtccat tctcaaattc acccctgagc cactcaccgt atataaattc
      661 tctttttgga ggatattgcc aagggacaac ttcactccta tttataaccg taacttcaag
      721 tggtctaaca gaatccgtat ttccaatctt tcctgatata gtcattagtc tttctgttag
      781 tttttttcga gttaattgag gtaaactatg attcacgacg actagaacat ctacatcgct
      841 gttaatgcgt aaaccaccat ttactgctga accaaataga tatactccaa ctattgaact
      901 tccaaataaa tcttttacga tttttaatgt ttgaatcgct tgatttggta tttttccgtt
      961 aatcaaattg ctcatgattt cacctcgttg attatgttca tataaagttt atattgatac
     1021 tcaatttact taccctagat tggacatata cttaaattac tgttcaataa agctgaccgt
     1081 tagcgtttaa gtacatcctt tcacaatttg tctacagatt aataattatt ctttattata
     1141 cagatcgatc ctctagagta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1201 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1261 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttcgaaa caaaacccac
     1321 tttttcactc gttaaaaagt tttgagaata ttttatattt ttgttcatgt aatcactcct
     1381 tcttaattac aaatttttag catctaattt aacttcaatt cctattatac aaaattttaa
     1441 gatactgcac tatcaacaca ctcttaagtt tgcttctaag tcttatttcc ataacttctt
     1501 ttacgtttcc gccattcttt gctgtttcga tttttatgat atggtgcaag tcagcacgaa
     1561 cacgaaccgt cttatctccc attatatctt tttttgcact gattggtgta tcatttcgtt
     1621 tttctttttg tgactctaga ggatcctgat aaatatgaac atgatgagtg atcgttaaat
     1681 ttatactgca atcggatgcg attattgaat aaaagatatg agagatttat ctaatttctt
     1741 ttttcttgta aaaaaagaaa gttcttaaag gttttatagt tttggtcgta gagcacacgg
     1801 tttaacgact taattacgaa gtaaataagt ctagtgtgtt agactttatg aaatctatat
     1861 acgtttatat atatttatta tccgattttt tattaaaacg tctcaaaatc gtttctgaga
     1921 cgttttagcg tttatttcgt ttagttatcg gcataatcgt taaaacaggc gttatcgtag
     1981 cgtaaaagcc cttgagcgta gcgtggcttt gcagcgaaga tgttgtctgt tagattatga
     2041 aagccgatga ctgaatgaaa taataagcgc agcgcccttc tatttcggtt ggaggaggct
     2101 caagggagta tgagggaatg aaattccctc atgggtttga ttttaaaaat tgcttgcaat
     2161 tttgccgagc ggtagcgctg gaaaattttt gaaaaaaatt tggaatttgg aaaaaaatgg
     2221 ggggaaagga agcgaatttt gcttccgtac tacgaccccc cattaagtgc cgagtgccaa
     2281 tttttgtgcc aaaaacgctc tatcccaact ggctcaaggg tttaaggggt ttttcaatcg
     2341 ccaacgaatc gccaacgttt tcgccaacgt tttttataaa tctatattta agtagcttta
     2401 ttgttgtttt tatgattaca aagtgataca ctaactttat aaaattattt gattggagtt
     2461 ttttaaatgg tgatttcaga atcgaaaaaa agagttatga tttctctgac aaaagagcaa
     2521 gataaaaaat taacagatat ggcgaaacaa aaaggttttt caaaatctgc ggttgcggcg
     2581 ttagctatag aagaatatgc aagaaaggaa tcagaacaaa aaaaataagc gaaagctcgc
     2641 gtttttagaa ggatacgagt tttcgctact tgtttttgat aaggtaatta tatcatggct
     2701 attaaaaata ctaaagctag aaattttgga tttttattat atcctgactc aattcctaat
     2761 gattggaaag aaaaattaga gagtttgggc gtatctatgg ctgtcagtcc tttacacgat
     2821 atggacgaaa aaaaagataa agatacatgg aataatagta atattataca aaatggaaag
     2881 cactataaaa aaccacacta tcacgttata tatattgcac gaaatcctgt aacaatagaa
     2941 agcgttagga acaagattaa gcgaaaattg gggaatagtt cagttgctca tgttgagata
     3001 cttgattata tcaaaggttc atatgaatat ttgactcatg aatcaaagga cgctattgct
     3061 aagaataaac atatatacga caaaaaagat attttgaaca ttaatgattt tgatattgac
     3121 cgctatataa cacttgatga aagccaaaaa agagaattga agaatttact tttagatata
     3181 gtggatgact ataatttggt aaatacaaaa gatttaatgg cttttattcg ccttagggga
     3241 gcggagtttg gaattttaaa tacgaatgat gtaaaagata ttgtttcaac aaactctagc
     3301 gcctttagat tatggtttga gggcaattat cagtgtggat atagagcaag ttatgcaaag
     3361 gttcttgatg ctgaaacggg ggaaataaaa tgacaaacaa agaaaaagag ttatttgctg
     3421 aaaatgagga attaaaaaaa gaaattaagg acttaaaaga gcgtattgaa agatacagag
     3481 aaatggaagt tgaattaagt acaacaatag atttattgag aggagggatt attgaataaa
     3541 taaaagcccc ctgacgaaag tcgaaggggg tttttatttt ggtttgatgt tgcgattaat
     3601 agcaatacaa ttgcaataaa caaaatgatc ttccttcagg ttatgaccat ctgtgccagt
     3661 tcgtaatgtc tggtcaactt tccgactctg agaaacttct ggaatcgcta gagaatttct
     3721 ggaatgggat tcaggagtgg acagaacgac acggatatat agtggatgtg tcaaaacgca
     3781 taccattttg aacgatgacc tctaataatt gttaatcatg ttggttacgt atttattaac
     3841 ttctcctagt attagtaatt atcatggctg tcatggcgca ttaacggaat aaagggtgtg
     3901 cttaaatcgg gccattttgc gtaataagaa aaaggattaa ttatgagcga attgaattaa
     3961 taataaggta atagatttac attagaaaat gaaaggggat tttatgcgtg agaatgttac
     4021 agtctatccc ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     4081 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     4141 atagggcgaa ttgggtaccg ggccccccct cgagaagctc gcgttatcgg tcctttaatt
     4201 ggtggacaaa tttacattag tctcggacat tctgctcccg cccttatggg atttatcttc
     4261 cttataactc aacaagtctc agtgtgctga agttttggga ccattcaaaa cagcatagct
     4321 ctaaaacgag acctttgagc ttccgagact ggtctcagtt ttgggaccat tcaaaacagc
     4381 atagctctaa aacctcgtag actatttttg tctaaaaaat ttcgtaatcg cactatttgt
     4441 ctcagctaga cttcagtctt gaaaagcccc tgtattactg catttattaa gagtattata
     4501 ccatattttt agttattaag aaatatctcg ctttgattgt tctatcgaaa gcgaaatcaa
     4561 acgaataatt tataaataaa aaaaccacct ctaaaaggtg atttttattt ataaattaca
     4621 atttttcagc aatatcagta attgctttat caactgctgc tttttggcta tcaatcaaag
     4681 caacacgtgc tgtaatttgt ttaattgcca tttcaattga acgtttcaag ccttggtttt
     4741 ctaattttgg ttcaaagaaa gcttgagctc tctagaacta gtggtaccgc atgcctgcag
     4801 tacccatggt gagtggaatt c
//
