LOCUS       sequence-291820-        3081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Orbit integrating plasmid to delete target gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Murphy KC, Nelson SJ, Nambi S, Papavinasasundaram K, Baer CE, 
            Sassetti CM
  TITLE     ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial 
            Chromosomes.
  JOURNAL   MBio. 2018 Dec 11;9(6). pii: mBio.01467-18. doi: 
            10.1128/mBio.01467-18.
  PUBMED    30538179
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2018-12-11"; volume: "9(6). pii: mBio.01467-18. doi"; pages: 
            " 10.1128/mBio.01467-18"
COMMENT     Sequence Label: pKM464 (deletions)
FEATURES             Location/Qualifiers
     source          1..3081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    47..84
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     rep_origin      complement(304..892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(384..403)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(1157..1176)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(3029..3075)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagtcgat cgtacgctag ttaactacgt cgacatcgat aagcttggct tgtcgacgac
       61 ggcggtctcc gtcgtcagga tcatctcgag tctagagcat gcactagtgc ggccgctagc
      121 ggtaccagat ctgggccctt aagtcgcgtt gcgctcggtc gttcggctgc ggcgagcggt
      181 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      241 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      301 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      361 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      421 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccctcggg
      481 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      541 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      601 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      661 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      721 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
      781 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      841 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      901 tttgatcttt tctacggggt ctgacgctca tggaactaga gcatgagatt atcaaaaagg
      961 atcttcacct agatcctttt aaattaaaaa tcggcacgta agaggttcca actttcacca
     1021 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     1081 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     1141 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     1201 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     1261 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     1321 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     1381 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     1441 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     1501 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     1561 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     1621 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1681 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggctggc
     1741 ggaaacggca atgctgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1801 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1861 ccatagttgc ctgactcccc gacgtggccg accagcccgt catcgtcaac gcctgatccg
     1921 cggtgcggac aggccgtgtc gcgaccggcc gtgcggaatt aagccggccc gtaccctgtg
     1981 aatagaggtc cgctgtgaca caagaatccc tgttacttct cgaccgtatt gattcggatg
     2041 attcctacgc gagcctgcgg aacgaccagg aattctggga gccgctggcc cgccgagccc
     2101 tggaggagct cgggctgccg gtgccgccgg tgctgcgggt gcccggcgag agcaccaacc
     2161 ccgtactggt cggcgagccc ggcccggtga tcaagctgtt cggcgagcac tggtgcggtc
     2221 cggagagcct cgcgtcggag tcggaggcgt acgcggtcct ggcggacgcc ccggtgccgg
     2281 tgccccgcct cctcggccgc ggcgagctgc ggcccggcac cggagcctgg ccgtggccct
     2341 acctggtgat gagccggatg accggcacca cctggcggtc cgcgatggac ggcacgaccg
     2401 accggaacgc gctgctcgcc ctggcccgcg aactcggccg ggtgctcggc cggctgcaca
     2461 gggtgccgct gaccgggaac accgtgctca ccccccattc cgaggtcttc ccggaactgc
     2521 tgcgggaacg ccgcgcggcg accgtcgagg accaccgcgg gtggggctac ctctcgcccc
     2581 ggctgctgga ccgcctggag gactggctgc cggacgtgga cacgctgctg gccggccgcg
     2641 aaccccggtt cgtccacggc gacctgcacg ggaccaacat cttcgtggac ctggccgcga
     2701 ccgaggtcac cgggatcgtc gacttcaccg acgtctatgc gggagactcc cgctacagcc
     2761 tggtgcaact gcatctcaac gccttccggg gcgaccgcga gatcctggcc gcgctgctcg
     2821 acggggcgca gtggaagcgg accgaggact tcgcccgcga actgctcgcc ttcaccttcc
     2881 tgcacgactt cgaggtgttc gaggagaccc cgctggatct ctccggcttc accgatccgg
     2941 aggaactggc gcagttcctc tgggggccgc cggacaccgc ccccggcgcc tgacgccccc
     3001 tctagctgat caccgcggcc atgatggcat aaaacgaaag gcccagtctt tcgactgagc
     3061 ctttcgtttt atttgatgcc t
//
