LOCUS       sequence-291832-        3931 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Subramanian R, Gelles J
  TITLE     Two Distinct Modes of Processive Kinesin Movement in Mixtures of ATP
            and AMP-PNP.
  JOURNAL   J Gen Physiol. 2007 Nov . 130(5):445-55.
  PUBMED    17968024
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Physiol. 2007 Nov . 130(5):445-55."
COMMENT     Sequence Label: pWC2
FEATURES             Location/Qualifiers
     source          1..3931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1493..1510
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(1592..2383,2384..2452))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2215..2234
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2453..2557)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2625..2643
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      2772..3360
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3261..3280
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3514..3531
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3766..3788
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3853..3872
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3853..3871
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3903..3925
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tatgtccgcg gaacgagaga ttcccgccga ggacagcatc aaagtggtct gccgattccg
       61 accgctgaac gacagcgaag agaaggccgg ctccaagttc gtggtcaagt tccccaacaa
      121 tgtggaggag aactgcatat ccatagcggg caaggtgtat ttgttcgaca aggtcttcaa
      181 accgaatgca tcccaggaaa aggtctacaa tgaggcggcc aagtccattg ttacggatgt
      241 cctggccggg tacaatggaa cgatattcgc atatggtcag acgtcctccg gaaaaacgca
      301 tacgatggag ggcgtgatcg gggactccgt aaaacagggt atcataccac gtatcgtcaa
      361 cgacattttc aatcacatct acgcgatgga ggtgaacctg gagtttcaca tcaaggtctc
      421 ctactacgag atctacatgg acaagattcg agatctgttg gacgtctcca aggtgaacct
      481 tagtgtgcac gaggataaga accgggtgcc gtacgtcaag ggcgctacgg aacggttcgt
      541 ctcgtcgccg gaggatgttt tcgaggtgat cgaggagggc aaatccaatc gtcacatcgc
      601 tgtgacaaac atgaacgagc attcttcgcg atcccactca gtattcctta tcaatgtgaa
      661 gcaggagaac ctggagaacc agaagaaact atccggcaaa ctctacctgg tggatttggc
      721 cggttccgag aaggtttcca agactggagc ggagggaacc gttcttgatg aagccaagaa
      781 catcaacaag tcgctgtcgg ccttgggcaa cgtaatttct gccctggcgg acggaaacaa
      841 aacgcacatc ccctaccgtg attccaagct aacgcgcatc ctgcaggagt cgctgggagg
      901 caacgcacgc acaaccatcg tcatctgctg ctctccagcc agtttcaacg aatctgaaac
      961 gaagtcaacg ctggacttcg gtcgtagagc caagacagtg aagaacgtgg tctgcgttaa
     1021 cgaggagctt actgccgagg aatggaagcg acgctatgaa aaggagaagg aaaagaacgc
     1081 ccgactaaag ggtaaggtgg agaagctgga gatcgagctt gcgcgctgga gagcgggtga
     1141 aactgttaag gcggaggagc aaatcaacat ggaggatctc atggaggcaa gcacgcccaa
     1201 cctgcgcaag gccatggaag cgccagcagc agcggaaatc agtggtcaca tcgtacgttc
     1261 cccgatggtt ggtactttct accgcacccc aagcccggac gcaaaagcgt tcatcgaagt
     1321 gggtcagaaa gtcaacgtgg gcgataccct gtgcatcgtt gaagccatga aaatgatgaa
     1381 ccagatcgaa gcggacaaat ccggtaccgt taaagctatc cttgttgaat ctggtcagcc
     1441 ggttgaattc gacgaaccgc ttgttgttat cgaattatct gagactagtg ggcaccacca
     1501 tcaccatcac tgacaagctt atcgatgata agctgtcaaa catgagaatt aaatcaatct
     1561 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1621 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1681 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1741 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1801 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1861 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1921 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1981 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2041 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2101 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2161 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     2221 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2281 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2341 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2401 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2461 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2521 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     2581 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     2641 ggccctttcg tcttcaagaa taaaaggatc taggtgaaga tcctttttga taatctcatg
     2701 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2761 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2821 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2881 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2941 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3001 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3061 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3121 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3181 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3241 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3301 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3361 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3421 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3481 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3541 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatagga
     3601 agatcttccg gaagatcttc ctatggtgca ctctcagtac aatctgctct gatgcgctac
     3661 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     3721 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     3781 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggcc cagcgattcg aacttctgat
     3841 agacttcgaa attaatacga ctcactatag ggagaccaca acggtttccc tctagaaata
     3901 attttgttta actttaagaa ggagatatac a
//
