LOCUS       sequence-291838-       10382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral EGFP lipid sensor for PI(4,5)P2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10382)
  TITLE     APEX2 and lentiviral lipid sensors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-PH-PLC-delta-EGFP
FEATURES             Location/Qualifiers
     source          1..10382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1197..1206
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1725..1734
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1731..1733,1734..1736,1737..2450)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1731..1733,1734..1736,1737..2447)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1776..1797)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2037..2056)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2384..2405
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2472..3060
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2525..2545)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2943..2954)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3584..3764
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3790..3807)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3796..4020
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4066..4494
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4153..4172)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4363..4384
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4503..4523)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4508..4837
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4688..4823
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4750..4769
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4885..4932
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4951..5325
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5455..5576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5492..5511)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5546..5565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5625..5641)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5625..5641)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5638..5660)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5649..5665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5673..5679,5680..5697,5698..5703))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5718..5739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5856..5873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6027..6615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6107..6126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6786..7577,7578..7646))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7409..7428
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7647..7751)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7826..7845)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8017..8396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8398..8596
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8551..8571
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8614..8794
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8841..8966
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9459..9692
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9877..9921
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10219..10336
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgtgg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattcggcc attacggcca
     1201 ccatggactc gggccgggac ttcctgaccc tgcacggcct acaggatgat gaggatctac
     1261 aggcgctgct gaagggcagc cagctcctga aggtgaagtc cagctcatgg aggagagagc
     1321 gcttctacaa gttgcaggag gactgcaaga ccatctggca ggagtcccgc aaggtcatgc
     1381 ggaccccgga gtcccagctg ttctccatcg aggacattca ggaggtgcga atggggcacc
     1441 gcacggaggg tctggagaag ttcgcccgtg atgtgcccga ggaccgctgc ttctccattg
     1501 tcttcaagga ccagcgcaat acactagacc tcatcgcccc atcgccagct gatgcccagc
     1561 actgggtgct ggggctgcac aagatcatcc accactcagg ctccatggac cagcgtcaga
     1621 agctacagca ctggattcac tcctgcttgc gaaaagctga caaaaacaag gacaacaaga
     1681 tgagcttcaa ggagctgcag aacttcctga aggatccacc ggtcgccacc atggtgagca
     1741 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1801 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1861 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1921 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1981 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     2041 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2101 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2161 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     2221 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     2281 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     2341 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2401 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa ggccgcctcg
     2461 gccgcggccg caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2521 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2581 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2641 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2701 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2761 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2821 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2881 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     2941 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3001 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3061 ctgcctgcag gtttgtcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     3121 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     3181 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     3241 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     3301 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     3361 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     3421 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     3481 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     3541 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     3601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     3661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     3721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     3781 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     3841 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     3901 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     3961 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     4021 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     4081 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4141 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4201 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4261 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4321 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4381 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4441 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     4501 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4561 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4621 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4681 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4741 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     4801 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     4861 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     4921 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     4981 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     5041 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     5101 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     5161 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     5221 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     5281 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     5341 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     5401 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     5461 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5521 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5581 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     5641 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5701 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5761 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5821 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5881 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5941 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6001 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6061 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6121 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6181 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6241 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6301 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6361 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6421 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6481 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6541 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6601 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6661 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6721 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6781 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6841 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6901 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6961 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7021 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7081 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7141 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7201 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7261 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7321 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7381 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7441 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7501 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7561 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7621 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7681 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7741 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     7801 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     7861 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     7921 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     7981 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     8041 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     8101 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     8161 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     8221 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     8281 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     8341 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     8401 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     8461 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     8521 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     8581 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
     8641 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8701 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8761 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     8821 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     8881 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     8941 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     9001 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     9061 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     9121 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     9181 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     9241 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     9301 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     9361 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     9421 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     9481 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     9541 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     9601 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     9661 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     9721 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     9781 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     9841 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     9901 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     9961 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    10021 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    10081 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    10141 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
    10201 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    10261 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    10321 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa
    10381 tt
//
