LOCUS       sequence-291842-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for sACE2-sfGFP high affinity variant 
            4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pcDNA3-sACE2v4-sfGFP
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    263..384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(300..319)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     354..373
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(433..449)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(433..449)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(446..468)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    457..473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(481..487,488..505,506..511))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    526..547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(664..681)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(915..934)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
       61 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
      121 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
      181 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
      241 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
      301 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      361 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
      421 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      481 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      541 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      601 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      661 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      721 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      781 agcaaaaggn cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      841 tnggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcana ggtggcgaaa
      901 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      961 tgttccgacc ctgc
//
