LOCUS       sequence-291845-        9284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminally strep tagged Kaposi's Sarcoma Associated 
            Herpesvirus (KSHV) ORF25.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9284)
  AUTHORS   Davis ZH, Verschueren E, Jang GM, Kleffman K, Johnson JR, Park J, 
            Von Dollen J, Maher MC, Johnson T, Newton W, Jager S, Shales M, 
            Horner J, Hernandez RD, Krogan NJ, Glaunsinger BA
  TITLE     Global mapping of herpesvirus-host protein complexes reveals a 
            transcription strategy for late genes.
  JOURNAL   Mol Cell. 2015 Jan 22;57(2):349-60. doi: 
            10.1016/j.molcel.2014.11.026. Epub 2014 Dec 24.
  PUBMED    25544563
REFERENCE   2  (bases 1 to 9284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.11.026"; journalName: "Mol Cell"; date: 
            "2015-01-22- 22"; volume: "57"; issue: "2"; pages: "349-60"
COMMENT     Sequence Label: pCDNA4.TO-ORF25-2xCSTREP
FEATURES             Location/Qualifiers
     source          1..9284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5183..5206
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5243..5266
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(5292..5309)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5298..5522
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5568..5996
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5655..5674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5865..5886
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6005..6025)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6010..6339
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6190..6325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6252..6271
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6387..6434
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6453..6827
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6957..7078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6994..7013)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7048..7067
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7127..7143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7127..7143)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7140..7162)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7151..7167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7175..7181,7182..7199,7200..7205))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7220..7241
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7358..7375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7529..8117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7609..7628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8288..9079,9080..9148))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8911..8930
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9149..9253)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     1021 attcatggag gcgaccttgg agcaacgacc tttcccgtac ctcgccacgg aggccaacct
     1081 cctaacgcag attaaggagt cggctgccga cggactcttc aagagctttc agctattgct
     1141 cggcaaggac gccagagaag gcagtgtccg tttcgaagcg ctactgggcg tatataccaa
     1201 tgtggtggag tttgttaagt ttctggagac cgccctcgcc gccgcttgcg tcaataccga
     1261 gttcaaggac ctgcggagaa tgatagatgg aaaaatacag tttaaaattt caatgcccac
     1321 tattgcccac ggagacggga ggaggcccaa caagcagaga cagtatatcg tcatgaaggc
     1381 ttgcaataag caccacatcg gtgcggagat tgagcttgcg gccgcagaca tcgagcttct
     1441 cttcgccgag aaagagacgc ccttggactt cacagagtac gcgggtgcca tcaagacgat
     1501 tacgtcggct ttgcagtttg gtatggacgc cctagaacgg gggctagtgg acacggttct
     1561 cgcagttaaa cttcggcacg ctccacccgt ctttatttta aagacgctgg gcgatcccgt
     1621 ctactctgag aggggcctca aaaaggccgt caagtctgac atggtatcca tgttcaaggc
     1681 acacctcata gaacattcat tttttctaga taaggccgag ctcatgacaa gggggaagca
     1741 gtatgtccta accatgctct ccgacatgct ggccgcggtg tgcgaggata ccgtctttaa
     1801 gggtgtcagc acgtacacca cggcctctgg gcagcaggtg gccggcgtcc tggagacgac
     1861 ggacagcgtc atgagacggc tgatgaacct gctggggcaa gtggaaagtg ccatgtccgg
     1921 gcccgcggcc tacgccagct acgttgtcag gggtgccaac ctcgtcaccg ccgttagcta
     1981 cggaagggcg atgagaaact ttgaacagtt tatggcacgc atagtggacc atcccaacgc
     2041 tctgccgtct gtggaaggtg acaaggccgc tctggcggac ggacacgacg agattcagag
     2101 aacccgcatc gccgcctctc tcgtcaagat aggggataag tttgtggcca ttgaaagttt
     2161 gcagcgcatg tacaacgaga ctcagtttcc ctgcccactg aaccggcgca tccagtacac
     2221 ctatttcttc cctgttggcc ttcaccttcc cgtgccccgc tactcgacat ccgtctcagt
     2281 caggggcgta gaatccccgg ccatccagtc gaccgagacg tgggtggtta ataaaaacaa
     2341 cgtgcctctt tgcttcggtt accaaaacgc cctcaaaagc atatgccacc ctcgaatgca
     2401 caaccccacc cagtcagccc aggcactaaa ccaagctttt cccgatcccg acgggggaca
     2461 tgggtacggt ctcaggtatg agcagacgcc aaacatgaac ctattcagaa cgttccacca
     2521 gtattacatg gggaaaaacg tggcatttgt tcccgatgtg gcccaaaaag cgctcgtaac
     2581 cacggaggat ctactgcacc caacctctca ccgtctcctc agattggagg tccacccctt
     2641 ctttgatttt tttgtgcacc cctgtcctgg agcgagagga tcgtaccgcg ccacccacag
     2701 aacaatggtt ggaaatatac cacaaccgct cgctccaagg gagtttcagg aaagtagagg
     2761 ggcgcagttc gacgctgtga cgaatatgac acacgtcata gaccagctaa ctattgacgt
     2821 catacaggag acggcatttg accccgcgta tcccctgttc tgctatgtaa tcgaagcaat
     2881 gattcacgga caggaagaaa aattcgtgat gaacatgccc ctcattgccc tggtcattca
     2941 aacctactgg gtcaactcgg gaaaactggc gtttgtgaac agttatcaca tggttagatt
     3001 catctgtacg catatgggga atggaagcat ccctaaggag gcgcacggcc actaccggaa
     3061 aatcttaggc gagctcatcg cccttgagca ggcgcttctc aagctcgcgg gacacgagac
     3121 ggtgggtcgg acgccgatca cacatctggt ttcggctctc ctcgacccgc atctgctgcc
     3181 tccctttgcc taccacgatg tctttacgga tcttatgcag aagtcatcca gacaacccat
     3241 aatcaagatc ggggatcaaa actacgacaa ccctcaaaat agggcgacat tcatcaacct
     3301 caggggtcgc atggaggacc tagtcaataa ccttgttaac atttaccaga caagggtcaa
     3361 tgaggaccat gacgagagac acgtcctgga cgtggcgccc ctggacgaga atgactacaa
     3421 cccggtcctc gagaagctat tctactatgt tttaatgccg gtgtgcagta acggccacat
     3481 gtgcggtatg ggggtcgact atcaaaacgt ggccctgacg ctgacttaca acggccccgt
     3541 ctttgcggac gtcgtgaacg cacaggatga tattctactg cacctggaga acggaacctt
     3601 gaaggacatt ctgcaggcag gcgacatacg cccgacggtg gacatgatca gggtgctgtg
     3661 cacctcgttt ctgacgtgcc ctttcgtcac ccaggccgct cgcgtgatca caaagcggga
     3721 cccggcccag agttttgcca cgcacgaata cgggaaggat gtggcgcaga ccgtgcttgt
     3781 taatggcttt ggtgcgttcg cggtggcgga ccgctctcgc gaggcggcgg agactatgtt
     3841 ttatccggta ccctttaaca agctctacgc tgacccgttg gtggctgcca cactgcatcc
     3901 gctcctggca aactatgtca ccaggctccc caaccagaga aacgcggtgg tctttaacgt
     3961 gccatccaat ctcatggcag aatatgagga atggcacaag tcgcccgtcg cggcgtatgc
     4021 cgcgtcttgt caggccaccc cgggcgccat tagcgccatg gtgagcatgc accaaaaact
     4081 atctgccccc agtttcattt gccaggcaaa acaccgcatg caccctggtt ttgccatgac
     4141 agtcgtcagg acggacgagg ttctagcaga gcacatccta tactgctcca gggcgtcgac
     4201 atccatgttt gtgggcttgc cttcggtggt acggcgcgag gtacgttcgg acgcggtgac
     4261 ttttgaaatt acccacgaga tcgcttccct gcacaccgca cttggctact catcagtcat
     4321 cgccccggcc cacgtggccg ccataactac agacatggga gtacattgtc aggacctctt
     4381 tatgattttc ccaggggacg cgtatcagga ccgccagctg catgactata tcaaaatgaa
     4441 agcgggcgtg caaaccggct caccgggaaa cagaatggat cacgtgggat acactgctgg
     4501 ggttcctcgc tgcgagaacc tgcccggttt gagtcatggt cagctggcaa cctgcgagat
     4561 aattcccacg ccggtcacat ctgacgttgc ctatttccag acccccagca acccccgggg
     4621 gcgtgcggcg tgcgtggtgt cgtgtgatgc ttacagtaac gaaagcgcag agcgtttgct
     4681 ctacgaccat tcaataccag accccgcgta cgaatgccgg tccaccaaca acccgtgggc
     4741 ttcgcagcgt ggctccctcg gcgacgtgct atacaatatc acctttcgcc agactgcgct
     4801 gccgggcatg tacagtcctt gtcggcagtt cttccacaag gaagacatta tgcggtacaa
     4861 tagggggttg tacactttgg ttaatgagta ttctgccagg cttgctgggg cccccgccac
     4921 cagcactaca gacctccagt acgtcgtggt caacggtaca gacgtgtttt tggaccagcc
     4981 ttgccatatg ctgcaggagg cctatcccac gctcgccgcc agccacagag ttatgcttga
     5041 cgagtacatg tcaaacaagc agacacacgc cccagtacac atgggccagt atctcattga
     5101 agaggtggcg ccgatgaaga gactattaaa gctcggaaac aaggtggtgt attcgccgcg
     5161 gccgctcgag ggaggcggtg gatggagcca cccccagttc gagaagggcg gcggcagcgg
     5221 cggcggcagc ggcggcggca gctggagcca cccccagttc gagaaggggc ccgtttaaac
     5281 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5341 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5401 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5461 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5521 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     5581 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5641 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5701 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5761 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5821 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5881 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     5941 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     6001 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     6061 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     6121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     6181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6241 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     6301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     6361 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     6421 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     6481 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     6541 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     6601 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     6661 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     6721 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     6781 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     6841 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6901 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6961 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7021 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7081 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     7141 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7201 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7261 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7321 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7381 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7441 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7501 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7561 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7621 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7681 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7741 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7801 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7861 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7921 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7981 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8041 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8101 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8161 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8221 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8281 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8341 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8401 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8461 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8521 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8581 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8641 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8701 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8761 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8821 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8881 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8941 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9001 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9061 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9121 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9181 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9241 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
