LOCUS       sequence-291847-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for sACE2-sfGFP high affinity variant 
            1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pcDNA3-sACE2v1-sfGFP
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..734)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(24..734)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(64..84)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     679..707
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gggncnctag actcgagtta tcatttgtag agctcatcca tgccatgtgt aatcccagca
       61 gcagttacaa actcaagaag gaccatgtgg tcacgctttt cgttgggatc tttcgaaagg
      121 acagattgtg tcgacaggta atggttgtct ggtaaaagga cagggccatc gccaattgga
      181 gtattttgtt gataatggtc tgctagttga acggaaccat cttcaacgtt gtggcgaatt
      241 ttgaagttag ctttgattcc attcttttgt ttgtctgccg tgatgtatac attgtgtgag
      301 ttaaagttgt actccagttt gtgtccgaga atgtttccat cttctttaaa atcaatacct
      361 tttaactcga tacgattaac aagggtatca ccttcaaact tgacttcagc acgcgtcttg
      421 taggtcccgt catctttgaa agatatagtg cgttcctgta cataaccttc gggcatggca
      481 ctcttgaaaa agtcatgccg tttcatgtga tccggataac gggaaaagca ttgaacacca
      541 taggtcagag tagtgacaag tgttggccat ggaacaggta gttttccagt agtgcaaata
      601 aatttaaggg tgagttttcc gtttgtagca tcaccttcac cctctccacg gacagaaaat
      661 ttgtgcccat taacatcacc atctaattca acaagaattg ggacaactcc agtgaaaagt
      721 tcttctcctt tggaaccacc actaccggac ccaccggatc cgtctgcata tggactccag
      781 tcggtactcc atcccacaaa agaattcttg ttctggtctt tcagccaggt aaataagggc
      841 tcaaagtagt tgagcagtgg ccttacattc atgttctttg ctcctacaa
//
