LOCUS       sequence-291848-        1248 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral EGFP lipid sensor for PI(4,5)P2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1248)
  TITLE     APEX2 and lentiviral lipid sensors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-PH-PLC-delta-EGFP
FEATURES             Location/Qualifiers
     source          1..1248
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      523..532
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(529..531,532..534,535..1248)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(529..531,532..534,535..1245)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(574..595)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(835..854)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1182..1203
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggactcgg gccgggactt cctgaccctg cacggcctac aggatgatga ggatctacag
       61 gcgctgctga agggcagcca gctcctgaag gtgaagtcca gctcatggag gagagagcgg
      121 ttctacaagt tgcaggagga ctgcaagacc atctggcagg agtcccgcaa ggtcatgcgg
      181 accccggagt cccagctgtt ctccatcgag gacattcagg aggtgcgaat ggggcaccgc
      241 acggagggtc tggagaagtt cgcccgtgat gtgcccgagg accgctgctt ctccattgtc
      301 ttcaaggacc agcgcaatac actagacctc atcgccccat cgccagctga tgcccagcac
      361 tgggtgctgg ggctgcacaa gatcatccac cactcaggct ccatggacca gcgtcagaag
      421 ctacagcact ggattcactc ctgcttgcga aaagctgaca aaaacaagga caacaagatg
      481 agcttcaagg agctgcagaa cttcctgaag gatccaccgg tcgccaccat ggtgagcaag
      541 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      601 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      661 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      721 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      781 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      841 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      901 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      961 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1021 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1081 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1141 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1201 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaa
//
