LOCUS       sequence-291851-        8192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminally strep tagged Kaposi's Sarcoma Associated 
            Herpesvirus (KSHV) ORF9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8192)
  AUTHORS   Davis ZH, Verschueren E, Jang GM, Kleffman K, Johnson JR, Park J, 
            Von Dollen J, Maher MC, Johnson T, Newton W, Jager S, Shales M, 
            Horner J, Hernandez RD, Krogan NJ, Glaunsinger BA
  TITLE     Global mapping of herpesvirus-host protein complexes reveals a 
            transcription strategy for late genes.
  JOURNAL   Mol Cell. 2015 Jan 22;57(2):349-60. doi: 
            10.1016/j.molcel.2014.11.026. Epub 2014 Dec 24.
  PUBMED    25544563
REFERENCE   2  (bases 1 to 8192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.11.026"; journalName: "Mol Cell"; date: 
            "2015-01-22- 22"; volume: "57"; issue: "2"; pages: "349-60"
COMMENT     Sequence Label: pCDNA4.TO-ORF9-2xCSTREP
FEATURES             Location/Qualifiers
     source          1..8192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4091..4114
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4151..4174
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(4200..4217)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4206..4430
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4476..4904
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4563..4582)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4773..4794
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4913..4933)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4918..5247
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5098..5233
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5160..5179
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5295..5342
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5361..5735
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5865..5986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5902..5921)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5956..5975
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6035..6051)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6035..6051)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6048..6070)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6059..6075
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6083..6089,6090..6107,6108..6113))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6128..6149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6266..6283)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6437..7025)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6517..6536)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7196..7987,7988..8056))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7819..7838
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8057..8161)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     1021 attcatggat tttttcaatc catttatcga cccaactcgc ggaggcccga gaaacactgt
     1081 gaggcaaccc acgccgtcac agtcgccaac tgtcccctcg gagacaagag tatgcaggct
     1141 tataccggcc tgtttccaaa ccccggggcg acccggcgtg gttgccgtgg acaccacatt
     1201 tccacccacc tacttccagg gccccaagcg gggagaagta ttcgcgggag agactgggtc
     1261 tatctggaaa acaaggcgcg gacaggcacg caatgctcct atgtcgcacc tcatattcca
     1321 cgtatacgac atcgtggaga ccacctacac ggccgaccgc tgcgaggacg tgccatttag
     1381 cttccagact gatatcattc ccagcggcac cgtcctcaag ctgctcggca gaacactaga
     1441 tggcgccagt gtctgcgtga acgttttcag gcagcgctgc tacttctaca cactagcacc
     1501 ccagggggta aacctgaccc acgtcctcca gcaggccctc caggctggct tcggtcgcgc
     1561 atcctgcggc ttctccaccg agccggtcag aaaaaaaatc ttgcgcgcgt acgacacaca
     1621 acaatatgct gtgcaaaaaa taaccctgtc atccagtccg atgatgcgaa cgcttagcga
     1681 ccgcctaaca acctgtgggt gcgaggtgtt tgagtccaat gtggacgcca ttaggcgctt
     1741 cgtgctggac cacgggttct cgacattcgg gtggtacaag tgcagcaacc cggccccccg
     1801 cacccaggcc agagactctt ggacggaact ggagtttgac tgcagctggg aggacctaaa
     1861 gtttatcccg gagaggacgg agtggccccc atacacaatc ctatcctttg atatagaatg
     1921 tatgggcgag aagggttttc ccaacgcgac tcaagacgag gacatgatta tacaaatctc
     1981 gtgtgtttta cacacagacg gcaacgataa accgtacacc cgcatgctac tgggcctagg
     2041 gacatgcgac ccccttcctg gggtggaggt ctttgagttt ccttcggagt acgacatgct
     2101 ggccgccttc ctcagcatgc tccgcgatta caatgtggag tttataacgg ggtacaacat
     2161 agcaaacttt gaccttccat acatcatagc ccgggcaact caggtgtacg acttcaagct
     2221 gcaggacttc accaaaataa aaactgggtc cgtgtttgag gtccaccaac ccagaggcgg
     2281 ttccgatggg ggcaacttca tgaggtccca gtcaaaggtc aaaatatcgg ggatcgtccc
     2341 catagacatg taccaggttt gcagggaaaa gctgagtctg tcagactaca agctggacac
     2401 agtggctaag caatgcctcg gtcgacaaaa agatgacatc tcatacaagg acataccccc
     2461 gctttttaaa tctggacctg atggtcgcgc aaaggtggga aactactgtg ttattgactc
     2521 ggtcctggtt atggatcttc tgctacggtt tcagacccac gttgagatct cggaaatagc
     2581 caagctggcc aagatcccca cccgtagggt actgacggac ggccaacaga tcagggtatt
     2641 ttcctgcctc ttggaggctg ctgccacgga aggttacatt ctccccgtcc caaaaggaga
     2701 cgcggttagc gggtatcagg gggccactgt aataagcccc tctccgggat tctatgacga
     2761 ccccgtactc gtggtggatt ttgccagctt gtaccccagt atcatccaag cgcacaactt
     2821 gtgctactcc acactgatac ccggcgattc gctccacctg cacccacacc tctccccgga
     2881 cgactacgaa acctttgtcc tcagcggagg tccggtccac tttgtaaaaa aacacaaaag
     2941 ggagtccctt cttgccaagc ttctgacggt atggctcgcg aagagaaaag aaataagaaa
     3001 gaccctggca tcatgcacgg accccgcact gaaaactatt ctagacaaac aacaactggc
     3061 catcaaggtt acctgcaacg ccgtttacgg cttcacgggc gttgcctctg gcatactgcc
     3121 ttgcctaaac atagcggaga ccgtgacact acaagggcga aagatgctgg agagatctca
     3181 ggcctttgta gaggccatct cgccggaacg cctagcgggt ctcctgcgga ggccagtaga
     3241 cgtctcaccc gacgcccgat tcaaggtcat atacggcgac actgactctc ttttcatatg
     3301 ctgcatgggt ttcaacatgg acagcgtgtc agacttcgcg gaggagctag cgtcaatcac
     3361 caccaacacg ctgtttcgta gccccatcaa gctggaggct gaaaagatct tcaagtgcct
     3421 tctgctcctg actaaaaaga gatacgtggg ggtactcagt gacgacaagg ttctgatgaa
     3481 gggcgtagac ctcattagga aaacagcctg tcgttttgtc caggaaaaga gcagtcaggt
     3541 cctggacctc atactgcggg agccgagcgt caaggccgcg gccaagctta tttcggggca
     3601 ggcgacagac tgggtgtaca gggaagggct cccagagggg ttcgtcaaga taattcaagt
     3661 gctcaacgcg agccaccggg aactgtgcga acgcagcgta ccagtagaca aactgacgtt
     3721 taccaccgag ctaagccgcc cgctggcgga ctacaagacg caaaacctcc cgcacctgac
     3781 cgtgtaccaa aagctacaag ctagacagga ggagcttccc cagatacacg acagaatccc
     3841 ctacgtgttc gtcgacgccc caggtagcct gcgctccgag ctggcagagc accccgagta
     3901 cgtcaagcag cacggactgc gcgtggcggt ggacctgtac ttcgacaagc tggtacacgc
     3961 ggtagccaac atcatccaat gcctcttcca gaacaacacg tcggcaaccg tagctatctt
     4021 gtataacttt ttagacattc ccgtgacttt tcccacgccc tcggggcggc cgctcgaggg
     4081 aggcggtgga tggagccacc cccagttcga gaagggcggc ggcagcggcg gcggcagcgg
     4141 cggcggcagc tggagccacc cccagttcga gaaggggccc gtttaaaccc gctgatcagc
     4201 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4261 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4321 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4381 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     4441 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     4501 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4561 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4621 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4681 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4741 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4801 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4861 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     4921 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4981 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     5041 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     5101 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5161 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     5221 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     5281 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     5341 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     5401 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     5461 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     5521 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     5581 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     5641 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     5701 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     5761 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     5821 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     5881 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5941 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     6001 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6061 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6121 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6181 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6241 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6301 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6361 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6421 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6481 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6541 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6601 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6661 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6721 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6781 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6841 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6901 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6961 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7021 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7081 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7141 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7201 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7261 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7321 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7381 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7441 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7501 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7561 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7621 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7681 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7741 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7801 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7861 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7921 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7981 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8041 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8101 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8161 gcacatttcc ccgaaaagtg ccacctgacg tc
//
