LOCUS       sequence-291863-        6821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  containing 1.3 units of the HBV genome (subtype ayw).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Wang H, Kim S, Ryu WS
  TITLE     DDX3 DEAD-Box RNA helicase inhibits hepatitis B virus reverse 
            transcription by incorporation into nucleocapsids.
  JOURNAL   J Virol. 2009 Jun;83(11):5815-24. doi: 10.1128/JVI.00011-09. Epub 
            2009 Mar 18.
  PUBMED    19297497
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.00011-09"; journalName: "J Virol"; date: "2009-06"; 
            volume: "83"; issue: "11"; pages: "5815-24"
COMMENT     Sequence Label: HBV 1.3-mer WT replicon
FEATURES             Location/Qualifiers
     source          1..6821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             843..1688
                     /codon_start=1
                     /product="middle (pre-S2/S) envelope protein of hepatitis B
                     virus"
                     /label=HBsAg (pre-S2/S)
                     /note="hepatitis B surface antigen"
                     /translation="MQWNSTTFHQTLQDPRVRGLYFPAGGSSSGTVNPVLTTASPLSSI
                     FSRIGDPALNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGTTVCL
                     GQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCP
                     LIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLWEWAS
                     ARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCLWVYI
                     "
     primer_bind     complement(2865..2884)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2866..2884)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2903..2919)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2903..2919)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2916..2938)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2927..2943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2951..2957,2958..2975,2976..2981))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2996..3017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3134..3151)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3305..3893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3385..3404)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4064..4855,4856..4924))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4687..4706
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4925..5029)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5097..5115
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5153..5175)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5275..5294
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5488..5510
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5502..5519
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5503..5519
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5527..5545
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5527..5544
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ccggagacta ctgttgttag acgacgaggc aggtccccta gaagaagaac tccctcgcct
       61 cgcagacgaa ggtctcaatc gccgcgtcgc agaagatctc aatctcggga atctcaatgt
      121 tagtattcct tggactcata aggtggggaa ctttactggg ctttattctt ctactgtacc
      181 tgtctttaat cctcattgga aaacaccatc ttttcctaat atacatttac accaagacat
      241 tatcaaaaaa tgtgaacagt ttgtaggccc actcacagtt aatgagaaaa gaagattgca
      301 attgattatg cctgccaggt tttatccaaa ggttaccaaa tatttaccat tggataaggg
      361 tattaaacct tattatccag aacatctagt taatcattac ttccaaacta gacactattt
      421 acacactcta tggaaggcgg gtatattata taagagagaa acaacacata gcgcctcatt
      481 ttgtgggtca ccatattctt gggaacaaga tctacagcat ggggcagaat ctttccacca
      541 gcaatcctct gggattcttt cccgaccacc agttggatcc agccttcaga gcaaacaccg
      601 caaatccaga ttgggacttc aatcccaaca aggacacctg gccagacgcc aacaaggtag
      661 gagctggagc attcgggctg ggtttcaccc caccgcacgg aggccttttg gggtggagcc
      721 ctcaggctca gggcatacta caaactttgc cagcaaatcc gcctcctgcc tccaccaatc
      781 gccagtcagg aaggcagcct accccgctgt ctccaccttt gagaaacact catcctcagg
      841 ccatgcagtg gaattccaca accttccacc aaactctgca agatcccaga gtgagaggcc
      901 tgtatttccc tgctggtggc tccagttcag gaacagtaaa ccctgttctg actactgcct
      961 ctcccttatc gtcaatcttc tcgaggattg gggaccctgc gctgaacatg gagaacatca
     1021 catcaggatt cctaggaccc cttctcgtgt tacaggcggg gtttttcttg ttgacaagaa
     1081 tcctcacaat accgcagagt ctagactcgt ggtggacttc tctcaatttt ctagggggaa
     1141 ctaccgtgtg tcttggccaa aattcgcagt ccccaacctc caatcactca ccaacctctt
     1201 gtcctccaac ttgtcctggt tatcgctgga tgtgtctgcg gcgttttatc atcttcctct
     1261 tcatcctgct gctatgcctc atcttcttgt tggttcttct ggactatcaa ggtatgttgc
     1321 ccgtttgtcc tctaattcca ggatcctcaa caaccagcac gggaccatgc cggacctgca
     1381 tgactactgc tcaaggaacc tctatgtatc cctcctgttg ctgtaccaaa ccttcggacg
     1441 gaaattgcac ctgtattccc atcccatcat cctgggcttt cggaaaattc ctatgggagt
     1501 gggcctcagc ccgtttctcc tggctcagtt tactagtgcc atttgttcag tggttcgtag
     1561 ggctttcccc cactgtttgg ctttcagtta tatggatgat gtggtattgg gggccaagtc
     1621 tgtacagcat cttgagtccc tttttaccgc tgttaccaat tttcttttgt ctttgggtat
     1681 acatttaaac cctaacaaaa caaagagatg gggttactct ctaaatttta tgggttatgt
     1741 cattggatgt tatgggtcct tgccacaaga acacatcata caaaaaatca aagaatgttt
     1801 tagaaaactt cctattaaca ggcctattga ttggaaagta tgtcaacgaa ttgtgggtct
     1861 tttgggtttt gctgcccctt ttacacaatg tggttatcct gcgttgatgc ctttgtatgc
     1921 atgtattcaa tctaagcagg ctttcacttt ctcgccaact tacaaggcct ttctgtgtaa
     1981 acaatacctg aacctttacc ccgttgcccg gcaacggcca ggtctgtgcc aagtgtttgc
     2041 tgacgcaacc cccactggct ggggcttggt catgggccat cagcgcatgc gtggaacctt
     2101 ttcggctcct ctgccgatcc atactgcgga actcctagcc gcttgttttg ctcgcagcag
     2161 gtctggagca aacattatcg ggactgataa ctctgttgtc ctatcccgca aatatacatc
     2221 gtttccatgg ctgctaggct gtgctgccaa ctggatcctg cgcgggacgt cctttgttta
     2281 cgtcccgtcg gcgctgaatc ctgcggacga cccttctcgg ggtcgcttgg gactctctcg
     2341 tccccttctc cgtctgccgt tccgaccgac cacggggcgc acctctcttt acgcggactc
     2401 cccgtctgtg ccttctcatc tgccggaccg tgtgcacttc gcttcacctc tgcacgtcgc
     2461 atggagacca ccgtgaacgc ccaccaaata ttgcccaagg tcttacataa gaggactctt
     2521 ggactctcag caatgtcaac gaccgacctt gaggcatact tcaaagactg tttgtttaaa
     2581 gactgggagg agttggggga ggagattagg ttaaaggtct ttgtactagg aggctgtagg
     2641 cataaattgg tctgcgcacc agcaccatgc aactttttca cctctgccta atcatctctt
     2701 gttcatgtcc tactgttcaa gcctccaagc tgtgccttgg gtggctttgg ggcatggaca
     2761 tcgaccctta taaagaattc ggagctactg tggagttact ctcgtttttg ccttctgact
     2821 tctttccttc agtacgagat cttctagata ccgcaagctt gtctccctat agtgagtcgt
     2881 attagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2941 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3001 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     3061 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     3121 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3181 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3241 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3301 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3361 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3421 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3481 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3541 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3601 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3661 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3721 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3781 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3841 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3901 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3961 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4021 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4081 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4141 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4201 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4261 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4321 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4381 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4441 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4501 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4561 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4621 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4681 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4741 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4801 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4861 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4921 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4981 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5041 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     5101 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     5161 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     5221 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     5281 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     5341 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     5401 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     5461 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     5521 aattggattt aggtgacact atagaatacg aattcgagct cgtattcaat ctaagcaggc
     5581 tttcactttc tcgccaactt acaaggcctt tctgtgtaaa caatacctga acctttaccc
     5641 cgttgcccgg caacggccag gtctgtgcca agtgtttgct gacgcaaccc ccactggctg
     5701 gggcttggtc atgggccatc agcgcatgcg tggaaccttt tcggctcctc tgccgatcca
     5761 tactgcggaa ctcctagccg cttgttttgc tcgcagcagg tctggagcaa acattatcgg
     5821 gactgataac tctgttgtcc tatcccgcaa atatacatcg tttccatggc tgctaggctg
     5881 tgctgccaac tggatcctgc gcgggacgtc ctttgtttac gtcccgtcgg cgctgaatcc
     5941 tgcggacgac ccttctcggg gtcgcttggg actctctcgt ccccttctcc gtctgccgtt
     6001 ccgaccgacc acggggcgca cctctcttta cgcggactcc ccgtctgtgc cttctcatct
     6061 gccggaccgt gtgcacttcg cttcacctct gcacgtcgca tggagaccac cgtgaacgcc
     6121 cactaaatat tgcccaaggt cttacataag aggactcttg gactctcagc aatgtcaacg
     6181 accgaccttg aggcatactt caaagactgt ttgtttaaag actgggagga gttgggggag
     6241 gagattaggt taaaggtctt tgtactagga ggctgtaggc ataaattggt ctgcgcacca
     6301 gcaccatgca actttttcac ctctgcctaa tcatctcttg ttcatgtcct actgttcaag
     6361 cctccaagct gtgccttggg tggctttggg gcatggacat cgacccttat aaagaatttg
     6421 gagctactgt ggagttactc tcgtttttgc cttctgactt ctttccttca gtacgagatc
     6481 ttctagatac cgcctcagct ctgtatcggg aagccttaga gtctcctgag cattgttcac
     6541 ctcaccatac tgcactcagg caagcaattc tttgctgggg ggaactaatg actctagcta
     6601 cctgggtggg tgttaatttg gaagatccag cgtctagaga cctagtagtc agttatgtca
     6661 acactaatat gggcctaaag ttcaggcaac tcttgtggtt tcacatttct tgtctcactt
     6721 ttggaagaga aacagttata gagtatttgg tgtctttcgg agtgtggatt cgcactcctc
     6781 cagcttatag accaccaaat gcccctatcc tatcaacact t
//
