LOCUS       sequence-291874-        8023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for c-myc-tagged mGluR3 C127A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8023)
  TITLE     Mammalian expression plasmids for production of metabotropic 
            glutamate receptor type 3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3_HLA-cmyc-GRM3 C127A
FEATURES             Location/Qualifiers
     source          1..8023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..120
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(2720..2737)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2726..2950
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2996..3424
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3083..3102)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3293..3314
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3433..3453)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3438..3767
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3618..3753
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3680..3699
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3834..4628
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3888..3907)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4498..4517
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4802..4923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4839..4858)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4893..4912
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4972..4988)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4972..4988)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4985..5007)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4996..5012
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5020..5026,5027..5044,5045..5050))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5065..5086
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5203..5220)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5374..5962)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5454..5473)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6133..6924,6925..6993))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6756..6775
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6994..7098)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7173..7192)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7364..7743
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7744..7947
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7898..7918
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7992..8011
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7992..8010
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctagcgcca ccatggccgt catggcgccc cgaaccctcg tcctgctact ctcgggggcc
       61 ctggccctga cccagacctg ggcgggctcc gaacaaaagc ttatttctga agaggacttg
      121 ggaggatcca ggagagagat taaaatagaa ggtgaccttg ttttaggggg cctgtttcct
      181 attaacgaaa aaggcactgg aactgaagaa tgtgggcgaa tcaatgaaga ccgagggatt
      241 caacgcctgg aagccatgtt gtttgctatt gatgaaatca acaaagatga ttacttgcta
      301 ccaggagtga agttgggtgt tcacattttg gatacatgtt caagggatac ctatgcattg
      361 gagcaatcac tggagtttgt cagggcatct ttgacaaaag tggatgaagc tgagtatatg
      421 gctcctgatg gatcctatgc cattcaagaa aacatcccac ttctcattgc aggggtcatt
      481 ggtggctctt atagcagtgt ttccatacag gtggcaaacc tgctgcggct cttccagatc
      541 cctcagatca gctacgcatc caccagcgcc aaactcagtg ataagtcgcg ctatgattac
      601 tttgccagga ccgtgccccc cgacttctac caggccaaag ccatggctga gatcttgcgc
      661 ttcttcaact ggacctacgt gtccacagta gcctccgagg gtgattacgg ggagacaggg
      721 atcgaggcct tcgagcagga agcccgcctg cgcaacatct gcatcgctac ggcggagaag
      781 gtgggccgct ccaacatccg caagtcctac gacagcgtga tccgagaact gttgcagaag
      841 cccaacgcgc gcgtcgtggt cctcttcatg cgcagcgacg actcgcggga gctcattgca
      901 gccgccagcc gcgccaatgc ctccttcacc tgggtggcca gcgacggctg gggcgcgcag
      961 gagagcatca tcaagggcag cgagcatgtg gcctacggcg ccatcaccct ggagctggcc
     1021 tcccagcctg tccgccagtt cgaccgctac ttccagagcc tcaaccccta caacaaccac
     1081 cgcaacccct ggttccggga cttctgggag caaaagtttc agtgcagcct ccagaacaaa
     1141 cgcaaccaca ggcgcgtctg cgacaagcac ctggccatcg acagcagcaa ctacgagcaa
     1201 gagtccaaga tcatgtttgt ggtgaacgcg gtgtatgcca tggcccacgc tttgcacaaa
     1261 atgcagcgca ccctctgtcc caacactacc aagctttgtg atgctatgaa gatcctggat
     1321 gggaagaagt tgtacaagga ttacttgctg aaaatcaact tcacggctcc attcaaccca
     1381 aataaagatg cagatagcat agtcaagttt gacacttttg gagatggaat ggggcgatac
     1441 aacgtgttca atttccaaaa tgtaggtgga aagtattcct acttgaaagt tggtcactgg
     1501 gcagaaacct tatcgctaga tgtcaactct atccactggt cccggaactc agtccccact
     1561 tcccagtgca gcgacccctg tgcccccaat gaaatgaaga atatgcaacc aggggatgtc
     1621 tgctgctgga tttgcatccc ctgtgaaccg tacgaatacc tggctgatga gtttacctgt
     1681 atggattgtg ggtctggaca gtggcccact gcagacctaa ctggatgcta tgaccttcct
     1741 gaggactaca tcaggtggga agacgcctgg gccattggcc cagtcaccat tgcctgtctg
     1801 ggttttatgt gtacatgcat ggttgtaact gtttttatca agcacaacaa cacacccttg
     1861 gtcaaagcat cgggccgaga actctgctac atcttattgt ttggggttgg cctgtcatac
     1921 tgcatgacat tcttcttcat tgccaagcca tcaccagtca tctgtgcatt gcgccgactc
     1981 gggctgggga gttccttcgc tatctgttac tcagccctgc tgaccaagac aaactgcatt
     2041 gcccgcatct tcgatggggt caagaatggc gctcagaggc caaaattcat cagccccagt
     2101 tctcaggttt tcatctgcct gggtctgatc ctggtgcaaa ttgtgatggt gtctgtgtgg
     2161 ctcatcctgg aggccccagg caccaggagg tatacccttg cagagaagcg ggaaacagtc
     2221 atcctaaaat gcaatgtcaa agattccagc atgttgatct ctcttaccta cgatgtgatc
     2281 ctggtgatct tatgcactgt gtacgccttc aaaacgcgga agtgcccaga aaatttcaac
     2341 gaagctaagt tcataggttt taccatgtac accacgtgca tcatctggtt ggccttcctc
     2401 cctatatttt atgtgacatc aagtgactac agagtgcaga cgacaaccat gtgcatctct
     2461 gtcagcctga gtggctttgt ggtcttgggc tgtttgtttg cacccaaggt tcacatcatc
     2521 ctgtttcaac cccagaagaa tgttgtcaca cacagactgc acctcaacag gttcagtgtc
     2581 agtggaactg ggaccacata ctctcagtcc tctgcaagca cgtatgtgcc aacggtgtgc
     2641 aatgggcggg aagtcctcga ctccaccacc tcatctctgt gactcgagtc tagagggccc
     2701 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     2761 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     2821 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     2881 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     2941 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     3001 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3061 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3121 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3181 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     3241 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3301 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     3361 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3421 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     3481 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3541 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3601 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3661 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     3721 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     3781 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     3841 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     3901 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     3961 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     4021 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     4081 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     4141 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     4201 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     4261 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     4321 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     4381 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     4441 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     4501 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     4561 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     4621 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     4681 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     4741 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     4801 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4861 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4921 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     4981 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     5041 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     5101 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5161 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5221 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5281 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5341 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5401 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5461 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5521 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5581 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5641 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5701 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5761 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     5821 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5881 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5941 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6001 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6061 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6121 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6181 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6241 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6301 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6361 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6421 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6481 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6541 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     6601 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     6661 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     6721 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     6781 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     6841 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     6901 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     6961 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7021 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7081 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     7141 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     7201 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     7261 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     7321 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     7381 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     7441 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     7501 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     7561 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     7621 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     7681 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     7741 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     7801 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     7861 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     7921 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
     7981 cttatcgaaa ttaatacgac tcactatagg gagacccaag ctg
//
