LOCUS       sequence-291875-       10468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Pietila I, Van Mourik D, Tamelander A, Kriz V, Claesson-Welsh L, 
            Tengholm A, Welsh M
  TITLE     Temporal Dynamics of VEGFA-Induced VEGFR2/FAK Co-Localization Depend
            on SHB.
  JOURNAL   Cells. 2019 Dec 15;8(12). pii: cells8121645. doi: 
            10.3390/cells8121645.
  PUBMED    31847469
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2019-12-15"; volume: "8(12). pii: cells8121645. doi"; pages: 
            " 10.3390/cells8121645"
COMMENT     Sequence Label: pcDNA3.1-Y1214F-VEGFR2-mCherry
FEATURES             Location/Qualifiers
     source          1..10468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5134..5844
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5279..5297)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5547..5566
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5890..5907)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5896..6120
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6166..6594
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6253..6272)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6463..6484
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6603..6623)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6608..6937
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6788..6923
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6850..6869
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6986..8011
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6986..8011
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8141..8262
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8178..8197)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8232..8251
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8311..8327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8311..8327)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8324..8346)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8335..8351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8359..8365,8366..8383,8384..8389))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8404..8425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8542..8559)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8713..9301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8793..8812)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9472..10263,10264..10332))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10095..10114
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10333..10437)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      961 agatatccag cacagtggcg gccgctcgag gtcgacgcgc catggagctc catggcgcgt
     1021 cgacgcgcca tggagaattc cagcaaggtg ctgctggccg tcgccctgtg gctctgcgtg
     1081 gagacccggg ccgcctctgt gggtttgcct agtgtttctc ttgatctgcc caggctcagc
     1141 atacaaaaag acatacttac aattaaggct aatacaactc ttcaaattac ttgcagggga
     1201 cagagggact tggactggct ttggcccaat aatcagagtg gcagtgagca aagggtggag
     1261 gtgactgagt gcagcgatgg cctcttctgt aagacactca caattccaaa agtgatcgga
     1321 aatgacactg gagcctacaa gtgcttctac cgggaaactg acttggcctc ggtcatttat
     1381 gtctatgttc aagattacag atctccattt attgcttctg ttagtgacca acatggagtc
     1441 gtgtacatta ctgagaacaa aaacaaaact gtggtgattc catgtctcgg gtccatttca
     1501 aatctcaacg tgtcactttg tgcaagatac ccagaaaaga gatttgttcc tgatggtaac
     1561 agaatttcct gggacagcaa gaagggcttt actattccca gctacatgat cagctatgct
     1621 ggcatggtct tctgtgaagc aaaaattaat gatgaaagtt accagtctat tatgtacata
     1681 gttgtcgttg tagggtatag gatttatgat gtggttctga gtccgtctca tggaattgaa
     1741 ctatctgttg gagaaaagct tgtcttaaat tgtacagcaa gaactgaact aaatgtgggg
     1801 attgacttca actgggaata cccttcttcg aagcatcagc ataagaaact tgtaaaccga
     1861 gacctaaaaa cccagtctgg gagtgagatg aagaaatttt tgagcacctt aactatagat
     1921 ggtgtaaccc ggagtgacca aggattgtac acctgtgcag catccagtgg gctgatgacc
     1981 aagaagaaca gcacatttgt cagggtccat gaaaaacctt ttgttgcttt tggaagtggc
     2041 atggaatctc tggtggaagc cacggtgggg gagcgtgtca gaatccctgc gaagtacctt
     2101 ggttacccac ccccagaaat aaaatggtat aaaaatggaa taccccttga gtccaatcac
     2161 acaattaaag cggggcatgt actgacgatt atggaagtga gtgaaagaga cacaggaaat
     2221 tacactgtca tccttaccaa tcccatttca aaggagaagc agagccatgt ggtctctctg
     2281 gttgtgtatg tcccacccca gattggtgag aaatctctaa tctctcctgt ggattcctac
     2341 cagtacggca ccactcaaac gctgacatgt acggtctatg ccattcctcc cccgcatcac
     2401 atccactggt attggcagtt ggaggaagag tgcgccaacg agcccagcca agctgtctca
     2461 gtgacaaacc catacccttg tgaagaatgg agaagtgtgg aggacttcca gggaggaaat
     2521 aaaattgaag ttaataaaaa tcaatttgct ctaattgaag gaaaaaacaa aactgtaagt
     2581 acccttgtta tccaagcggc aaatgtgtca gctttgtaca aatgtgaagc ggtcaacaaa
     2641 gtcgggagag gagagagggt gatctccttc cacgtgacca ggggtcctga aattactttg
     2701 caacctgaca tgcagcccac tgagcaggag agcgtgtctt tgtggtgcac tgcagacaga
     2761 tctacgtttg agaacctcac atggtacaag cttggcccac agcctctgcc aatccatgtg
     2821 ggagagttgc ccacacctgt ttgcaagaac ttggatactc tttggaaatt gaatgccacc
     2881 atgttctcta atagcacaaa tgacattttg atcatggagc ttaagaatgc atccttgcag
     2941 gaccaaggag actatgtctg ccttgctcaa gacaggaaga ccaagaaaag acattgcgtg
     3001 gtcaggcagc tcacagtcct agagcgtgtg gcacccacga tcacaggaaa cctggagaat
     3061 cagacgacaa gtattgggga aagcatcgaa gtctcatgca cggcatctgg gaatccccct
     3121 ccacagatca tgtggtttaa agataatgag acccttgtag aagactcagg cattgtattg
     3181 aaggatggga accggaacct cactatccgc agagtgagga aggaggacga aggcctctac
     3241 acctgccagg catgcagtgt tcttggctgt gcaaaagtgg aggcattttt cataatagaa
     3301 ggtgcccagg aaaagacgaa cttggaaatc attattctag taggcacggc ggtgattgcc
     3361 atgttcttct ggctacttct tgtcatcatc ctacggaccg ttaagcgggc caatggaggg
     3421 gaactgaaga caggctactt gtccatcgtc atggatccag atgaactccc attggatgaa
     3481 cattgtgaac gactgcctta tgatgccagc aaatgggaat tccccagaga ccggctgaag
     3541 ctaggtaagc ctcttggccg tggtgccttt ggccaagtga ttgaagcaga tgcctttgga
     3601 attgacaaga cagcaacttg caggacagta gcagtcaaaa tgttgaaaga aggagcaaca
     3661 cacagtgagc atcgagctct catgtctgaa ctcaagatcc tcattcatat tggtcaccat
     3721 ctcaatgtgg tcaaccttct aggtgcctgt accaagccag gagggccact catggtgatt
     3781 gtggaattct gcaaatttgg aaacctgtcc acttacctga ggagcaagag aaatgaattt
     3841 gtcccctaca agaccaaagg ggcacgattc cgtcaaggga aagactacgt tggagcaatc
     3901 cctgtggatc tgaaacggcg cttggacagc atcaccagta gccagagctc agccagctct
     3961 ggatttgtgg aggagaagtc cctcagtgat gtagaagaag aggaagctcc tgaagatctg
     4021 tataaggact tcctgacctt ggagcatctc atctgttaca gcttccaagt ggctaagggc
     4081 atggagttct tggcatcgcg aaagtgtatc cacagggacc tggcggcacg aaatatcctc
     4141 ttatcggaga agaacgtggt taaaatctgt gactttggct tggcccggga tatttataaa
     4201 gatccagatt atgtcagaaa aggagatgct cgcctccctt tgaaatggat ggccccagaa
     4261 acaatttttg acagagtgta cacaatccag agtgacgtct ggtcttttgg tgttttgctg
     4321 tgggaaatat tttccttagg tgcttctcca tatcctgggg taaagattga tgaagaattt
     4381 tgtaggcgat tgaaagaagg aactagaatg agggcccctg attatactac accagaaatg
     4441 taccagacca tgctggactg ctggcacggg gagcccagtc agagacccac gttttcagag
     4501 ttggtggaac atttgggaaa tctcttgcaa gctaatgctc agcaggatgg caaagactac
     4561 attgttcttc cgatatcaga gactttgagc atggaagagg attctggact ctctctgcct
     4621 acctcacctg tttcctgtat ggaggaggag gaagtatgtg accccaaatt ccattttgac
     4681 aacacagcag gaatcagtca gtatctgcag aacagtaagc gaaagagccg gcctgtgagt
     4741 gtaaaaacat ttgaagatat cccgttagaa gaaccagaag taaaagtaat cccagatgac
     4801 aaccagacgg acagtggtat ggttcttgcc tcagaagagc tgaaaacttt ggaagacaga
     4861 accaaattat ctccatcttt tggtggaatg gtgcccagca aaagcaggga gtctgtggca
     4921 tctgaaggct caaaccagac aagcggctac cagtccggat atcactccga tgacacagac
     4981 accaccgtgt actccagtga ggaagcagaa cttttaaagc tgatagagat tggagtgcaa
     5041 accggtagca cagcccagat tctccagcct gactcgggga ccacactgag ctctcctcct
     5101 gttggatccg gtgggagtgg aggtgggagt ggaatggtga gcaagggcga ggaggataac
     5161 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     5221 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     5281 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     5341 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     5401 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     5461 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     5521 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     5581 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     5641 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     5701 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     5761 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     5821 ggcatggacg agctgtacaa gtaaagcggc cgctcgagtc tagagggccc gtttaaaccc
     5881 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     5941 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6001 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6061 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6121 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6181 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6241 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6301 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6361 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     6421 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6481 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6541 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     6601 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6661 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6721 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6781 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6841 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     6901 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     6961 cattttcgga tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtctgtcga
     7021 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     7081 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     7141 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     7201 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
     7261 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     7321 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     7381 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     7441 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     7501 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     7561 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     7621 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     7681 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     7741 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     7801 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     7861 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     7921 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     7981 ccccagcact cgtccgaggg caaaggaata gcacgtgcta cgagatttcg attccaccgc
     8041 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     8101 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     8161 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8221 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     8281 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8341 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8401 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8461 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8521 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8581 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8641 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8701 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8761 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8821 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8881 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8941 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9001 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9061 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9121 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     9181 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9241 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9301 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9361 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9421 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9481 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9541 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9601 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9661 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9721 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9781 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9841 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9901 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9961 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10021 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10081 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10141 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10201 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10261 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10321 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10381 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10441 atttccccga aaagtgccac ctgacgtc
//
