LOCUS       sequence-291878-        9438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of TYK2 protein mutated in the two tyrosines 
            (Y1054F/Y1055F) of the activation loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Gauzzi MC, Velazquez L, McKendry R, Mogensen KE, Fellous M, 
            Pellegrini S
  TITLE     Interferon-alpha-dependent activation of Tyk2 requires 
            phosphorylation of positive regulatory tyrosines by another kinase.
  JOURNAL   J Biol Chem. 1996 Aug 23;271(34):20494-500. doi: 
            10.1074/jbc.271.34.20494.
  PUBMED    8702790
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.271.34"; journalName: "J Biol Chem"; date: "1996-08-23-
            23"; volume: "271"; issue: "34"; pages: "20494-500"
COMMENT     Sequence Label: pRC/CMV-TYK21054-5FF-VSV
FEATURES             Location/Qualifiers
     source          1..9438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3895..3911)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3895..3911)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(3921..3939)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3922..3939)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3959..3976)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3965..4189
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4235..4748
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4322..4341)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4532..4553
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4771..4791)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4776..5105
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4956..5091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5172..5966
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5226..5245)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5836..5855
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6140..6274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6177..6196)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6231..6250
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6305..6321)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6305..6321)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6318..6340)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6329..6345
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6353..6359,6360..6377,6378..6383))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6398..6419
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6536..6553)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6707..7295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6787..6806)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7466..8257,8258..8326))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8089..8108
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8327..8431)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8506..8525)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8697..9076
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9077..9280
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9231..9251
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9325..9344
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9325..9343
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccccccaaga agccgcgggg actggctgcg cttgacaggc tgcacttgga tgggagcacc
       61 tggtgcctcg ggactgctcc gatgcccggg tctgtgctga atgtgtaata tgcggaacta
      121 tattgaaaca ttacaaccat cttttgatgg caacaccctg aggacctccc ttttccagat
      181 ggggaaactg aggcccagaa ttgctaagtg gcttgcttga gttgacacag ggagctccag
      241 gactcaccct cagctgagcc acctgccggg agcatgcctc tgcgccactg ggggatggcc
      301 aggggcagta agcccgttgg ggatggagcc cagcccatgg ctgccatggg aggcctgaag
      361 gtgcttctgc actgggctgg tccaggcggc ggggagccct gggtcacttt cagtgagtca
      421 tcgctgacag ctgaggaagt ctgcatccac attgcacata aagttggtat cactcctcct
      481 tgcttcaatc tctttgccct cttcgatgct caggcccaag tctggttgcc cccaaaccac
      541 atcctagaga tccccagaga tgcaagcctg atgctatatt tccgcataag gttttatttc
      601 cggaactggc atggcatgaa tcctcgggaa ccggctgtgt accgttgtgg gcccccagga
      661 accgaggcat cctcagatca gacagcacag gggatgcaac tcctggaccc agcctcattt
      721 gagtacctct ttgagcaggg caagcatgag tttgtgaatg acgtggcatc actgtgggag
      781 ctgtcgaccg aggaggagat ccaccacttt aagaatgaga gcctgggcat ggcctttctg
      841 cacctctgtc acctcgctct ccgccatggc atccccctgg aggaggtggc caagaagacc
      901 agcttcaagg actgcatccc gcgctccttc cgccggcata tccggcagca cagcgccctg
      961 acccggctgc gccttcggaa cgtcttccgc aggttcctgc gggacttcca gccgggccga
     1021 ctctcccagc agatggtcat ggtcaaatac ctagccacac tcgagcggct ggcaccccgc
     1081 ttcggcacag agcgtgtgcc cgtgtgccac ctgaggctgc tggcccaggc cgagggggag
     1141 ccctgctaca tccgggacag tggggtggcc cctacagacc ctggccctga gtctgctgct
     1201 gggcccccaa cccacgaggt gctggtgaca ggcactggtg gcatccagtg gtggccagta
     1261 gaggaggagg tgaacaagga ggagggttct agtggcagca gtggcaggaa cccccaagcc
     1321 agcctgtttg ggaagaaggc caaggctcac aaggcattcg gccagccggc agacaggccg
     1381 cgggagccac tgtgggccta cttctgtgac ttccgggaca tcacccacgt ggtgctgaaa
     1441 gagcactgtg tcagcatcca ccggcaggac aacaagtgcc tggagctgag cttgccttcc
     1501 cgggctgcgg cgctgtcctt cgtgtcgctg gtggacggct atttccgcct gacggccgac
     1561 tccagccact acctgtgcca cgaggtggct cccccacggc tggtgatgag catccgggat
     1621 gggatccacg gacccctgct ggagccattt gtgcaggcca agctgcggcc cgaggacggc
     1681 ctgtacctca ttcactggag caccagccac ccctaccgcc tgatcctcac agtggcccag
     1741 cgtagccagg caccagacgg catgcagagc ttgcggctcc gaaagttccc cattgagcag
     1801 caggacgggg ccttcgtgct ggagggctgg ggccggtcct tccccagcgt tcgggaactt
     1861 ggggctgcct tgcagggctg cttgctgagg gccggggatg actgcttctc tctgcgtcgc
     1921 tgttgcctgc cccaaccagg agaaacctcc aatctcatca tcatgcgggg ggctcgggcc
     1981 agccccagga cactcaacct cagccagctc agcttccacc gggttgacca gaaggagatc
     2041 acccagctgt cccacttggg ccagggcaca aggaccaacg tgtatgaggg ccgcctgcga
     2101 gtggagggca gcggggaccc tgaggagggc aagatggatg acgaggaccc cctcgtgcct
     2161 ggcagggacc gtgggcagga gctacgagtg gtgctcaaag tgctggaccc tagtcaccat
     2221 gacatcgccc tggccttcta cgagacagcc agcctcatga gccaggtctc ccacacgcac
     2281 ctggccttcg tgcatggcgt ctgtgtgcgc ggccctgaaa atatcatggt gacagagtac
     2341 gtggagcacg gacccctgga tgtgtggctg cggagggagc ggggccatgt gcccatggct
     2401 tggaagatgg tggtggccca gcagctggcc agcgccctca gctacctgga gaacaagaac
     2461 ctggttcatg gtaatgtgtg tggccggaac atcctgctgg cccggctggg gttggcagag
     2521 ggcaccagcc ccttcatcaa gctgagtgat cctggcgtgg gcctgggcgc cctctccagg
     2581 gaggagcggg tggagaggat cccctggctg gcccccgaat gcctaccagg tggggccaac
     2641 agcctaagca ccgccatgga caagtggggg tttggcgcca ccctcctgga gatctgcttt
     2701 gacggagagg cccctctgca gagccgcagt ccctccgaga aggagcattt ctaccagagg
     2761 cagcaccggc tgcccgagcc ctcctgccca cagctggcca cactcaccag ccagtgtctg
     2821 acctatgagc caacccagag gccatcattc cgcaccatcc tgcgtgacct cacccggctg
     2881 cagccccaca atcttgctga cgtcttgact gtgaacccgg actcaccggc gtcggaccct
     2941 acggttttcc acaagcgcta tttgaaaaag atccgagatc tgggcgaggg tcacttcggc
     3001 aaggtcagct tgtactgcta cgatccgacc aacgacggca ctggcgagat ggtggcggtg
     3061 aaagccctca aggcagactg cggcccccag caccgctcgg gctggaagca ggagattgac
     3121 attctgcgca cgctctacca cgagcacatc atcaagtaca agggctgctg cgaggaccaa
     3181 ggcgagaagt cgctgcagct ggtcatggag tacgtgcccc tgggcagcct ccgagactac
     3241 ctgccccggc acagcatcgg gctggcccag ctgctgctct tcgcccagca gatctgcgag
     3301 ggcatggcct atctgcacgc gcagcactac atccaccgag acctagccgc gcgcaacgtg
     3361 ctgctggaca acgacaggct ggtcaagatc ggggactttg gcctagccaa ggccgtgccc
     3421 gaaggccacg agttcttccg cgtgcgcgag gatggggaca gccccgtgtt ctggtatgcc
     3481 ccagagtgcc tgaaggagta taagttctac tatgcgtcag atgtctggtc cttcggggtg
     3541 accctgtatg agctgctgac gcactgtgac tccagccaga gcccccccac gaaattcctt
     3601 gagctcatag gcattgctca gggtcagatg acagttctga gactcactga gttgctggaa
     3661 cgaggggaga ggctgccacg gcccgacaaa tgtccctgtg aggtctatca tctcatgaag
     3721 aactgctggg agacagaggc gtcctttcgc ccaaccttcg agaacctcat acccattctg
     3781 aagacagtcc atgagaagta ccaaggccag gccccttcag tgttcagcgt gtgctgaggc
     3841 acaatggcag ccctgcctgg gaggactgga ccaggcagtg gctgcagccc gggggatcca
     3901 ctagttctag agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc
     3961 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     4021 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     4081 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4141 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     4201 gaaagaacca gctggggctc gaggggggat ccccacgcgc cctgtagcgg cgcattaagc
     4261 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4321 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4381 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4441 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4501 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4561 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4621 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4681 cttacaattt aaatatttgc ttatacaatc ttcctgtttt tggggctttt ctgattatca
     4741 accggggtgg gtaccgagct cgaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     4801 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4861 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4921 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4981 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     5041 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     5101 gcaaaaagct cccgggagct tggatatcca ttttcggatc tgatcaagag acaggatgag
     5161 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     5221 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5281 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     5341 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     5401 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     5461 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     5521 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     5581 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     5641 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     5701 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     5761 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     5821 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     5881 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     5941 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     6001 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     6061 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     6121 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     6181 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6241 caaactcatc aatgtatctt atcatgtctg gatccgtcga cctcgagagc ttggcgtaat
     6301 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6361 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6421 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6481 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6541 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6601 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6661 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6721 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6781 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6841 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6901 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6961 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7021 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7081 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7141 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7201 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7261 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7321 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7381 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7441 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7501 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7561 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7621 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7681 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7741 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7801 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7861 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7921 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7981 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8041 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8101 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8161 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8221 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8281 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8341 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8401 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8461 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     8521 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     8581 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     8641 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
     8701 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     8761 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     8821 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     8881 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     8941 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     9001 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     9061 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     9121 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     9181 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     9241 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
     9301 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttgtcg
     9361 aggaactgaa aaaccagaaa gttaactggt aagtttagtc tttttgtctt ttatttcagg
     9421 tcccggatcc cccccccc
//
