LOCUS       sequence-291881-        8589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of TYK2 protein missing the kinase-like (pseudokinase) 
            domain .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Velazquez L, Mogensen KE, Barbieri G, Fellous M, Uze G, Pellegrini S
  TITLE     Distinct domains of the protein tyrosine kinase tyk2 required for 
            binding of interferon-alpha/beta and for signal transduction.
  JOURNAL   J Biol Chem. 1995 Feb 17;270(7):3327-34. doi: 
            10.1074/jbc.270.7.3327.
  PUBMED    7531704
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1074/jbc.270.7";
            journalName: "J Biol Chem"; date: "1995-02-17- 17"; volume: "270"; 
            issue: "7"; pages: "3327-34"
COMMENT     Sequence Label: pRC/CMV-TYK2(delta)KL-VSV
FEATURES             Location/Qualifiers
     source          1..8589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3015..3047
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     promoter        complement(3082..3100)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3083..3100)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3120..3137)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3126..3350
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3396..3909
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3483..3502)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3693..3714
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3932..3952)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3937..4266
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4117..4252
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4333..5127
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4387..4406)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4997..5016
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5301..5435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5338..5357)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5392..5411
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5466..5482)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5466..5482)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5479..5501)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5490..5506
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5514..5520,5521..5538,5539..5544))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5559..5580
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5697..5714)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5868..6456)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5948..5967)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6627..7418,7419..7487))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7250..7269
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7488..7592)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7667..7686)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7858..8237
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8238..8441
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8392..8412
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8486..8505
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8486..8504
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cccccccccc caagaagccg cggggactgg ctgcgcttga caggctgcac ttggatggga
       61 gcacctggtg cctcgggact gctccgatgc ccgggtctgt gctgaatgtg taatatgcgg
      121 aactatattg aaacattaca accatctttt gatggcaaca ccctgaggac ctcccttttc
      181 cagatgggga aactgaggcc cagaattgct aagtggcttg cttgagttga cacagggagc
      241 tccaggactc accctcagct gagccacctg ccgggagcat gcctctgcgc cactggggga
      301 tggccagggg cagtaagccc gttggggatg gagcccagcc catggctgcc atgggaggcc
      361 tgaaggtgct tctgcactgg gctggtccag gcggcgggga gccctgggtc actttcagtg
      421 agtcatcgct gacagctgag gaagtctgca tccacattgc acataaagtt ggtatcactc
      481 ctccttgctt caatctcttt gccctcttcg atgctcaggc ccaagtctgg ttgcccccaa
      541 accacatcct agagatcccc agagatgcaa gcctgatgct atatttccgc ataaggtttt
      601 atttccggaa ctggcatggc atgaatcctc gggaaccggc tgtgtaccgt tgtgggcccc
      661 caggaaccga ggcatcctca gatcagacag cacaggggat gcaactcctg gacccagcct
      721 catttgagta cctctttgag cagggcaagc atgagtttgt gaatgacgtg gcatcactgt
      781 gggagctgtc gaccgaggag gagatccacc actttaagaa tgagagcctg ggcatggcct
      841 ttctgcacct ctgtcacctc gctctccgcc atggcatccc cctggaggag gtggccaaga
      901 agaccagctt caaggactgc atcccgcgct ccttccgccg gcatatccgg cagcacagcg
      961 ccctgacccg gctgcgcctt cggaacgtct tccgcaggtt cctgcgggac ttccagccgg
     1021 gccgactctc ccagcagatg gtcatggtca aatacctagc cacactcgag cggctggcac
     1081 cccgcttcgg cacagagcgt gtgcccgtgt gccacctgag gctgctggcc caggccgagg
     1141 gggagccctg ctacatccgg gacagtgggg tggcccctac agaccctggc cctgagtctg
     1201 ctgctgggcc cccaacccac gaggtgctgg tgacaggcac tggtggcatc cagtggtggc
     1261 cagtagagga ggaggtgaac aaggaggagg gttctagtgg cagcagtggc aggaaccccc
     1321 aagccagcct gtttgggaag aaggccaagg ctcacaaggc attcggccag ccggcagaca
     1381 ggccgcggga gccactgtgg gcctacttct gtgacttccg ggacatcacc cacgtggtgc
     1441 tgaaagagca ctgtgtcagc atccaccggc aggacaacaa gtgcctggag ctgagcttgc
     1501 cttcccgggc tgcggcgctg tccttcgtgt cgctggtgga cggctatttc cgcctgacgg
     1561 ccgactccag ccactacctg tgccacgagg tggctccccc acggctggtg atgagcatcc
     1621 gggatgggat ccacggaccc ctgctggagc catttgtgca ggccaagctg cggcccgagg
     1681 acggcctgta cctcattcac tggagcacca gccaccccta ccgcctgatc ctcacagtgg
     1741 cccagcgtag ccaggcacca gacggcatgc agagcttgcg gctccgaaag ttccccattg
     1801 agcagcagga cggggccttc gtgctggagg gctggggccg gtccttcccc agcgttcggg
     1861 aacttggggc tgccttgcag ggctgcttgc tgagggccgg ggatgactgc ttctctctgc
     1921 gtcgctgttg cctgccccaa ccaggagaaa cctccaatct catcatcatg cggggggctc
     1981 gggccagccc caggacactc aacctcagcc agctcagctt ccaccgggtt gaccagaagg
     2041 agatcaccca gaattcgatc ttgactgtga acccggactc accggcgtcg gaccctacgg
     2101 ttttccacaa gcgctatttg aaaaagatcc gagatctggg cgagggtcac ttcggcaagg
     2161 tcagcttgta ctgctacgat ccgaccaacg acggcactgg cgagatggtg gcggtgaaag
     2221 ccctcaaggc agactgcggc ccccagcacc gctcgggctg gaagcaggag attgacattc
     2281 tgcgcacgct ctaccacgag cacatcatca agtacaaggg ctgctgcgag gaccaaggcg
     2341 agaagtcgct gcagctggtc atggagtacg tgcccctggg cagcctccga gactacctgc
     2401 cccggcacag catcgggctg gcccagctgc tgctcttcgc ccagcagatc tgcgagggca
     2461 tggcctatct gcacgcgcag cactacatcc accgagacct agccgcgcgc aacgtgctgc
     2521 tggacaacga caggctggtc aagatcgggg actttggcct agccaaggcc gtgcccgaag
     2581 gccacgagta ctaccgcgtg cgcgaggatg gggacagccc cgtgttctgg tatgccccag
     2641 agtgcctgaa ggagtataag ttctactatg cgtcagatgt ctggtccttc ggggtgaccc
     2701 tgtatgagct gctgacgcac tgtgactcca gccagagccc ccccacgaaa ttccttgagc
     2761 tcataggcat tgctcagggt cagatgacag ttctgagact cactgagttg ctggaacgag
     2821 gggagaggct gccacggccc gacaaatgtc cctgtgaggt ctatcatctc atgaagaact
     2881 gctgggagac agaggcgtcc tttcgcccaa ccttcgagaa cctcataccc attctgaaga
     2941 cagtccatga gaagtaccaa ggccaggccc cttcagtgtt cagcgtgtgc ccccgggagg
     3001 gcccaccagg cccatacacc gacatcgaga tgaaccggct gggcaagtaa agatctaagg
     3061 taccaatcta gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc
     3121 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     3181 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     3241 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     3301 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     3361 ggaaagaacc agctggggct cgagggggga tccccacgcg ccctgtagcg gcgcattaag
     3421 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3481 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3541 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3601 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3661 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3721 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     3781 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     3841 gcttacaatt taaatatttg cttatacaat cttcctgttt ttggggcttt tctgattatc
     3901 aaccggggtg ggtaccgagc tcgaattctg tggaatgtgt gtcagttagg gtgtggaaag
     3961 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4021 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4081 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4141 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4201 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4261 tgcaaaaagc tcccgggagc ttggatatcc attttcggat ctgatcaaga gacaggatga
     4321 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     4381 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     4441 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     4501 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4561 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     4621 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     4681 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4741 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     4801 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     4861 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     4921 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     4981 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     5041 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     5101 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     5161 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     5221 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     5281 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     5341 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5401 ccaaactcat caatgtatct tatcatgtct ggatccgtcg acctcgagag cttggcgtaa
     5461 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5521 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5581 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5641 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5701 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5761 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5821 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5881 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5941 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6001 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6061 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6121 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6181 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6241 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6301 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6361 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6421 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6481 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6541 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6601 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6661 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6721 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6781 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6841 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6901 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6961 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7021 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7081 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7141 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7201 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7261 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7321 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7381 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7441 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7501 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7561 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7621 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     7681 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     7741 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     7801 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     7861 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     7921 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     7981 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     8041 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     8101 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     8161 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     8221 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     8281 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     8341 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     8401 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     8461 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagcttgtc
     8521 gaggaactga aaaaccagaa agttaactgg taagtttagt ctttttgtct tttatttcag
     8581 gtcccggat
//
