LOCUS       sequence-291883-        9821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human IKZF1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 9821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-IKZF1
FEATURES             Location/Qualifiers
     source          1..9821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    264..381
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(438..456,457..479,480..498,499..521,522..540,541..563,
                     564..582,583..605,606..624,625..647,648..666,667..689,
                     690..708)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        741..779
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     776..800
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2337..2925
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2390..2410)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2808..2819)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2928..2944)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2929..2945)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3450..3630
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3656..3673)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3662..3886
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3932..4360
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4019..4038)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4229..4250
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4369..4389)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4374..4703
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4554..4689
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4616..4635
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4751..4798
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4817..5191
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5321..5442
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5358..5377)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5412..5431
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5491..5507)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5491..5507)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5504..5526)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5515..5531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5539..5545,5546..5563,5564..5569))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5584..5605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5722..5739)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5893..6481)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5973..5992)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6652..7443,7444..7512))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7275..7294
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7513..7617)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7692..7711)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7883..8262
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8264..8462
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8480..8660
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8707..8832
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9325..9558
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9743..9787
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
       61 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
      121 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
      181 acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga
      241 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
      301 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
      361 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta
      421 gatctcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
      481 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
      541 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
      601 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
      661 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
      721 gagctcggta cccgggtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc
      781 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
      841 gacaccggga ccgatccagc ctccgcggcc ccgaattcat ggatgctgat gagggtcaag
      901 acatgtccca agtttcaggg aaggaaagcc cccctgtaag cgatactcca gatgagggcg
      961 atgagcccat gccgatcccc gaggacctct ccaccacctc gggaggacag caaagctcca
     1021 agagtgacag agtcgtggcc agtaatgtta aagtagagac tcagagtgat gaagagaatg
     1081 ggcgtgcctg tgaaatgaat ggggaagaat gtgcggagga tttacgaatg cttgatgcct
     1141 cgggagagaa aatgaatggc tcccacaggg accaaggcag ctcggctttg tcgggagttg
     1201 gaggcattcg acttcctaac ggaaaactaa agtgtgatat ctgtgggatc atttgcatcg
     1261 ggcccaatgt gctcatggtt cacaaaagaa gccacactgg agaacggccc ttccagtgca
     1321 atcagtgcgg ggcctcattc acccagaagg gcaacctgct ccggcacatc aagctgcatt
     1381 ccggggagaa gcccttcaaa tgccacctct gcaactacgc ctgccgccgg agggacgccc
     1441 tcactggcca cctgaggacg cactccgtca ttaaagaaga aactaatcac agtgaaatgg
     1501 cagaagacct gtgcaagata ggatcagaga gatctctcgt gctggacaga ctagcaagta
     1561 acgtcgccaa acgtaagagc tctatgcctc agaaatttct tggggacaag ggcctgtccg
     1621 acacgcccta cgacagcagc gccagctacg agaaggagaa cgaaatgatg aagtcccacg
     1681 tgatggacca agccatcaac aacgccatca actacctggg ggccgagtcc ctgcgcccgc
     1741 tggtgcagac gcccccgggc ggttccgagg tggtcccggt catcagcccg atgtaccagc
     1801 tgcacaagcc gctcgcggag ggcaccccgc gctccaacca ctcggcccag gacagcgccg
     1861 tggagaacct gctgctgctc tccaaggcca agttggtgcc ctcggagcgc gaggcgtccc
     1921 cgagcaacag ctgccaagac tccacggaca ccgagagcaa caacgaggag cagcgcagcg
     1981 gtctcatcta cctgaccaac cacatcgccc cgcacgcgcg caacgggctg tcgctcaagg
     2041 aggagcaccg cgcctacgac ctgctgcgcg ccgcctccga gaactcgcag gacgcgctcc
     2101 gcgtggtcag caccagcggg gagcagatga aggtgtacaa gtgcgaacac tgccgggtgc
     2161 tcttcctgga tcacgtcatg tacaccatcc acatgggctg ccacggcttc cgtgatcctt
     2221 ttgagtgcaa catgtgcggc taccacagcc aggaccggta cgagttctcg tcgcacataa
     2281 cgcgagggga gcaccgcttc cacatgagct aagaattcga tatcaagctt atcgataatc
     2341 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     2401 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     2461 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     2521 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     2581 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     2641 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     2701 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     2761 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     2821 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     2881 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     2941 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     3001 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     3061 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     3121 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     3181 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     3241 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     3301 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     3361 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     3421 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     3481 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     3541 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     3601 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     3661 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3721 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3781 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3841 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3901 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     3961 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4021 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4081 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4141 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4201 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4261 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4321 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     4381 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4441 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4501 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4561 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4621 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     4681 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     4741 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     4801 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     4861 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     4921 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     4981 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     5041 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     5101 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     5161 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     5221 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     5281 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     5341 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5401 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     5461 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5521 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     5581 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     5641 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5701 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5761 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5821 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5881 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5941 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6001 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6061 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6121 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6181 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6241 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6301 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6361 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6421 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6481 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6541 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6601 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6661 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6721 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6781 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6841 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6901 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6961 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7021 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7081 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7141 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7201 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7261 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7321 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7381 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7441 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7501 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7561 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7621 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     7681 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     7741 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     7801 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     7861 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     7921 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     7981 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     8041 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     8101 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     8161 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     8221 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     8281 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     8341 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     8401 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     8461 agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8521 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8581 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8641 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     8701 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     8761 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     8821 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     8881 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     8941 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     9001 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     9061 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     9121 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     9181 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     9241 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     9301 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     9361 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     9421 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     9481 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     9541 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     9601 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     9661 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     9721 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     9781 aagtttgtgg aattggttta acataacaaa ttggctgtgg t
//
