LOCUS       sequence-291885-        9677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human RUNX3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 9677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-RUNX3
FEATURES             Location/Qualifiers
     source          1..9677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    167..284
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(341..359,360..382,383..401,402..424,425..443,444..466,
                     467..485,486..508,509..527,528..550,551..569,570..592,
                     593..611)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        644..682
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     679..703
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1854..1863
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2096..2684
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2149..2169)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2567..2578)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2687..2703)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2688..2704)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3209..3389
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3415..3432)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3421..3645
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3691..4119
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3778..3797)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3988..4009
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4128..4148)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4133..4462
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4313..4448
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4375..4394
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4510..4557
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4576..4950
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5080..5201
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5117..5136)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5171..5190
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5250..5266)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5250..5266)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5263..5285)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5274..5290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5298..5304,5305..5322,5323..5328))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5343..5364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5481..5498)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5652..6240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5732..5751)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6411..7202,7203..7271))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7034..7053
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7272..7376)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7451..7470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7642..8021
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8023..8221
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8239..8419
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8466..8591
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9084..9317
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9502..9546
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
       61 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
      121 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      181 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      241 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      301 acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa
      361 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      421 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
      481 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      541 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      601 agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg
      661 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
      721 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt
      781 catggcatcg aacagcatct tcgactcctt cccgacctac tcgccgacct tcatccgcga
      841 cccaagcacc agccgccgct tcacacctcc ctccccggcc ttcccctgcg gcggcggcgg
      901 cggcaagatg ggcgagaaca gcggcgcgct gagcgcgcag gcggccgtgg ggcccggagg
      961 gcgcgcccgg cccgaggtgc gctcgatggt ggacgtgctg gcggaccacg caggcgagct
     1021 cgtgcgcacc gacagcccca acttcctctg ctccgtgctg ccctcgcact ggcgctgcaa
     1081 caagacgctg cccgtcgcct tcaaggtggt ggcattgggg gacgtgccgg atggtacggt
     1141 ggtgactgtg atggcaggca atgacgagaa ctactccgct gagctgcgca atgcctcggc
     1201 cgtcatgaag aaccaggtgg ccaggttcaa cgaccttcgc ttcgtgggcc gcagtgggcg
     1261 agggaagagt ttcaccctga ccatcactgt gttcaccaac cccacccaag tggcgaccta
     1321 ccaccgagcc atcaaggtga ccgtggacgg accccgggag cccagacggc accggcagaa
     1381 gctggaggac cagaccaagc cgttccctga ccgctttggg gacctggaac ggctgcgcat
     1441 gcgggtgaca ccgagcacac ccagcccccg aggctcactc agcaccacaa gccacttcag
     1501 cagccagccc cagaccccaa tccaaggcac ctcggaactg aacccattct ccgacccccg
     1561 ccagtttgac cgctccttcc ccacgctgcc aaccctcacg gagagccgct tcccagaccc
     1621 caggatgcat tatcccgggg ccatgtcagc tgccttcccc tacagcgcca cgccctcggg
     1681 cacgagcatc agcagcctca gcgtggcggg catgccggcc accagccgct tccaccatac
     1741 ctacctcccg ccaccctacc cgggggcccc gcagaaccag agcgggccct tccaggccaa
     1801 cccgtccccc taccacctct actacgggac atcctctggc tcctaccagt tctccatggt
     1861 ggccggcagc agcagtgggg gcgaccgctc acctacccgc atgctggcct cttgcaccag
     1921 cagcgctgcc tctgtcgccg ccggcaacct catgaacccc agcctgggcg gccagagtga
     1981 tggcgtggag gccgacggca gccacagcaa ctcacccacg gccctgagca cgccaggccg
     2041 catggatgag gccgtgtggc ggccctactg agaattcgat atcaagctta tcgataatca
     2101 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     2161 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     2221 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     2281 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     2341 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     2401 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     2461 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     2521 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     2581 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     2641 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     2701 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     2761 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     2821 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     2881 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     2941 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     3001 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     3061 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     3121 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     3181 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     3241 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     3301 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     3361 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
     3421 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     3481 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     3541 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     3601 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     3661 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     3721 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3781 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3841 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3901 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3961 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4021 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4081 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     4141 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4201 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4261 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4321 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4381 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     4441 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     4501 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     4561 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     4621 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     4681 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     4741 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     4801 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     4861 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     4921 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     4981 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     5041 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     5101 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     5161 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     5221 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     5281 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     5341 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     5401 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5461 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5521 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5581 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5641 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5701 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5761 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5821 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5881 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5941 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6001 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6061 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6121 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6181 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6241 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6301 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6361 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6421 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6481 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6541 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6601 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6661 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6721 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6781 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6841 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6901 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6961 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7021 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7081 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7141 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7201 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7261 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7321 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7381 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     7441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     7501 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     7561 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     7621 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     7681 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     7741 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     7801 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     7861 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     7921 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     7981 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     8041 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     8101 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     8161 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     8221 gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8281 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8341 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8401 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     8461 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     8521 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     8581 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     8641 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     8701 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     8761 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     8821 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     8881 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     8941 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     9001 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     9061 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     9121 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     9181 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     9241 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     9301 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     9361 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     9421 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     9481 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     9541 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     9601 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     9661 gttaggcagg gatattc
//
