LOCUS       sequence-291905-       10268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human NR4A3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 10268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-NR4A3
FEATURES             Location/Qualifiers
     source          1..10268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    167..284
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(341..359,360..382,383..401,402..424,425..443,444..466,
                     467..485,486..508,509..527,528..550,551..569,570..592,
                     593..611)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        644..682
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     679..703
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1064..1093
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1067..1096
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1070..1099
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1073..1102
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1076..1105
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     misc_feature    2687..3275
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2740..2760)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3158..3169)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3278..3294)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3279..3295)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3800..3980
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4006..4023)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4012..4236
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4282..4710
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4369..4388)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4579..4600
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4719..4739)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4724..5053
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4904..5039
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4966..4985
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5101..5148
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5167..5541
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5671..5792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5708..5727)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5762..5781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5841..5857)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5841..5857)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5854..5876)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5865..5881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5889..5895,5896..5913,5914..5919))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5934..5955
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6072..6089)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6243..6831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6323..6342)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7002..7793,7794..7862))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7625..7644
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7863..7967)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8042..8061)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8233..8612
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8614..8812
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8830..9010
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9057..9182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9675..9908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10093..10137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
       61 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
      121 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      181 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      241 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      301 acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa
      361 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      421 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
      481 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      541 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      601 agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg
      661 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
      721 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt
      781 catgccctgc gtccaagccc aatatagccc ttcccctcca ggttccagtt atgcggcgca
      841 gacatacagc tcggaataca ccacggagat catgaacccc gactacacca agctgaccat
      901 ggaccttggc agcactgaga tcacggctac agccaccacg tccctgccca gcatcagtac
      961 cttcgtggag ggctactcga gcaactacga actcaagcct tcctgcgtgt accaaatgca
     1021 gcggcccttg atcaaagtgg aggaggggcg ggcgcccagc taccatcacc atcaccacca
     1081 ccaccaccac caccaccacc atcaccagca gcagcatcag cagccatcca ttcctccagc
     1141 ctccagcccg gaggacgagg tgctgcccag cacctccatg tacttcaagc agtccccacc
     1201 gtccaccccc accacgccgg ccttcccccc gcaggcgggg gcgttatggg acgaggcact
     1261 gccctcggcg cccggctgca tcgcacccgg cccgctgctg gacccgccga tgaaggcggt
     1321 ccccacggtg gccggcgcgc gcttcccgct cttccacttc aagccctcgc cgccgcatcc
     1381 ccccgcgccc agcccggccg gcggccacca cctcggctac gacccgacgg ccgctgccgc
     1441 gctcagcctg ccgctgggag ccgcagccgc cgcgggcagc caggccgccg cgcttgaggg
     1501 ccacccgtac gggctgccgc tggccaagag ggcggccccg ctggccttcc cgcctctcgg
     1561 cctcacgccc tcccctaccg cgtccagcct gctgggcgag agtcccagcc tgccgtcgcc
     1621 gcccagcagg agctcgtcgt ctggcgaggg cacgtgtgcc gtgtgcgggg acaacgccgc
     1681 ctgccagcac tacggcgtgc gaacctgcga gggctgcaag ggctttttca agagaacagt
     1741 gcagaaaaat gcaaaatatg tttgcctggc aaataaaaac tgcccagtag acaagagacg
     1801 tcgaaaccga tgtcagtact gtcgatttca gaagtgtctc agtgttggaa tggtaaaaga
     1861 agttgtccgt acagatagtc tgaaagggag gagaggtcgt ctgccttcca aaccaaagag
     1921 cccattacaa caggaacctt ctcagccctc tccaccttct cctccaatct gcatgatgaa
     1981 tgctcttgtc cgagctttaa cagactcaac acccagagat cttgattatt ccagatactg
     2041 tcccactgac caggctgctg caggcacaga tgctgagcat gtgcaacaat tctacaacct
     2101 cctgacagcc tccattgatg tatccagaag ctgggcagaa aagattccgg gatttactga
     2161 tctccccaaa gaagatcaga cattacttat tgaatcagcc tttttggagc tgtttgtcct
     2221 cagactttcc atcaggtcaa acactgctga agataagttt gtgttctgca atggacttgt
     2281 cctgcatcga cttcagtgcc ttcgtggatt tggggagtgg ctcgactcta ttaaagactt
     2341 ttccttaaat ttgcagagcc tgaaccttga tatccaagcc ttagcctgcc tgtcagcact
     2401 gagcatgatc acagaaagac atgggttaaa agaaccaaag agagtcgaag agctatgcaa
     2461 caagatcaca agcagtttaa aagaccacca gagtaaggga caggctctgg agcccaccga
     2521 gtccaaggtc ctgggtgccc tggtagaact gaggaagatc tgcaccctgg gcctccagcg
     2581 catcttctac ctgaagctgg aagacttggt gtctccacct tccatcattg acaagctctt
     2641 cctggacacc ctacctttct aagaattcga tatcaagctt atcgataatc aacctctgga
     2701 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2761 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2821 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     2881 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     2941 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     3001 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     3061 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     3121 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     3181 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     3241 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct
     3301 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     3361 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     3421 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     3481 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     3541 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     3601 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     3661 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     3721 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     3781 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     3841 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     3901 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     3961 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     4021 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4081 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4141 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     4201 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     4261 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4321 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4381 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4441 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4501 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4561 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4621 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4681 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     4741 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4801 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4861 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4921 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4981 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5041 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     5101 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     5161 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     5221 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     5281 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     5341 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     5401 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     5461 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     5521 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
     5581 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     5641 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     5701 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5761 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     5821 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     5881 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     5941 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6001 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6061 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6121 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6181 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6241 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6301 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6361 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6421 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6481 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6541 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6601 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6661 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6721 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6781 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6841 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6901 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6961 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7021 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7081 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7141 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7201 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7261 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7321 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7381 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7441 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7501 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7561 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7621 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7681 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7741 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7801 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7861 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7921 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7981 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     8041 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     8101 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     8161 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     8221 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     8281 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     8341 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     8401 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     8461 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     8521 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     8581 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     8641 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     8701 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     8761 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt
     8821 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8881 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8941 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     9001 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     9061 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     9121 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     9181 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     9241 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     9301 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     9361 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     9421 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     9481 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     9541 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     9601 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     9661 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     9721 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     9781 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     9841 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     9901 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     9961 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    10021 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    10081 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    10141 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    10201 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    10261 ggatattc
//
