LOCUS       sequence-291910-        9859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of mouse Meis in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-Meis1
FEATURES             Location/Qualifiers
     source          1..9859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(2..20,21..43,44..62,63..85,86..104,105..127,128..146,
                     147..169,170..188,189..211,212..230,231..253,254..272)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        305..343
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     340..364
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1939..2527
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1992..2012)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2410..2421)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2530..2546)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2531..2547)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3052..3232
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3258..3275)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3264..3488
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3534..3962
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3621..3640)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3831..3852
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3971..3991)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3976..4305
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4156..4291
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4218..4237
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4353..4400
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4419..4793
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4923..5044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4960..4979)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5014..5033
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5093..5109)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5093..5109)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5106..5128)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5117..5133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5141..5147,5148..5165,5166..5171))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5186..5207
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5324..5341)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5495..6083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5575..5594)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6254..7045,7046..7114))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6877..6896
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7115..7219)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7294..7313)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7485..7864
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7866..8064
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8082..8262
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8309..8434
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8927..9160
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9345..9389
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9687..9804
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
       61 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      121 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      181 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      241 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc ggtacccggg
      301 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
      361 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      421 cagcctccgc ggccccgaat tcatggcgca aaggtacgac gacctacccc attatggggg
      481 tatggatgga gtaggcatcc cctccacgat gtatggggac ccgcatgcag ccaggtccat
      541 gcaaccggtc caccacctga accacgggcc tcctctgcac tcgcatcagt acccgcacac
      601 agctcacacc aacgccatgg cccccagcat gggttcctcg gtcaatgacg ctttaaagag
      661 agataaagat gccatttatg gacaccccct cttccctctc ttagcactga tttttgagaa
      721 atgtgaatta gctacttgta ccccccgcga gccgggggtg gcgggcgggg acgtctgctc
      781 gtcagagtca ttcaatgaag atatagcggt gttcgccaaa cagattcgcg cagaaaaacc
      841 tctattctct tctaatccag aactggataa cttgatgatt caagccatac aagtgttaag
      901 gtttcatctg ttggaattag agaaggtaca cgaattatgt gacaatttct gccaccggta
      961 tattagctgt ttgaaaggga aaatgcctat cgatttggtg atagatgata gagaaggagg
     1021 atcaaaatca gacagtgaag atgtaacaag atcagcaaat ctaactgacc agccctcttg
     1081 gaatagagac catgatgaca cggcatccac tcgttcagga ggaaccccgg gcccttccag
     1141 cggtggccat acttcacaca gtggggataa cagcagtgag caaggtgatg gcttggacaa
     1201 cagtgtagct tcccccagca caggtgacga tgatgaccct gataaggaca aaaagcgtca
     1261 caaaaagcgt ggcatctttc ccaaagtagc caccaatatc atgagggcgt ggctgttcca
     1321 gcatctaaca cacccttacc cttctgaaga acagaaaaag cagttggcac aagatacagg
     1381 actcaccatc cttcaagtga acaattggtt tattaatgcc cggagaagaa tagtgcagcc
     1441 catgatagac cagtccaacc gagcaggcaa gtcccctcta gtgactgtat tcaagtcagg
     1501 caagcgaaaa gcatcctcaa gccattcacc aggagggctg ctgcctggta aatgaactgg
     1561 gagtgagtac tacgagtgcc tggctttaga aatgaaacca gtttggtttt gtaaccacac
     1621 taatcaattt aacatcatta taaaatattt gaagaaggta gagaataaaa agccatagga
     1681 gaaaagtaac tcttaaacca ccaagtgctc agctacaacc ttctttgtaa acattcaata
     1741 tattaatgtt atttttcccc caagtttgcc tacccagctt tctgccacta tgaccactcc
     1801 ctggtgcatg gcttggtgtg gaattgctgt aaactttatt acctgtcaaa agggcaaagg
     1861 ggtcgggatc acttgtggga ctcagtaaag ttcaaagaca ttcacgctca gtaggaattc
     1921 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     1981 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2041 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2101 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2161 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2221 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2281 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     2341 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     2401 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     2461 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     2521 tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc
     2581 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     2641 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     2701 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     2761 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     2821 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     2881 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     2941 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     3001 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     3061 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     3121 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     3181 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     3241 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3301 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3361 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     3421 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     3481 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     3541 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3601 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3661 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3721 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3781 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3841 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3901 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3961 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4021 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4081 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4141 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4201 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     4261 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     4321 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     4381 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     4441 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     4501 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     4561 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     4621 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     4681 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     4741 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     4801 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     4861 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     4921 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4981 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5041 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     5101 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     5161 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     5221 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     5281 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     5341 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5401 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5461 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5521 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5581 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5641 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5701 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5761 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5821 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5881 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     5941 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6001 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6061 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6121 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6181 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6241 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6301 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6361 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6421 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6481 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6541 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6601 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6661 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6721 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6781 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6841 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     6901 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6961 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7021 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7081 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7141 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7201 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     7261 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7321 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     7381 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     7441 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
     7501 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     7561 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     7621 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     7681 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     7741 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     7801 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     7861 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     7921 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     7981 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     8041 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
     8101 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8161 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8221 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     8281 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     8341 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     8401 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     8461 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     8521 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     8581 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     8641 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     8701 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     8761 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     8821 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     8881 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     8941 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     9001 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     9061 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     9121 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     9181 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     9241 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     9301 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     9361 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     9421 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     9481 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     9541 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     9601 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     9661 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     9721 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     9781 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     9841 ttagatctcg agtttacca
//
