LOCUS       sequence-291940-        9509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human NFE2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-NFE2
FEATURES             Location/Qualifiers
     source          1..9509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    167..284
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(341..359,360..382,383..401,402..424,425..443,444..466,
                     467..485,486..508,509..527,528..550,551..569,570..592,
                     593..611)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        644..682
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     679..703
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1928..2516
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1981..2001)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2399..2410)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2519..2535)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2520..2536)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3041..3221
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3247..3264)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3253..3477
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3523..3951
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3610..3629)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3820..3841
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3960..3980)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3965..4294
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4145..4280
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4207..4226
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4342..4389
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4408..4782
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4912..5033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4949..4968)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5003..5022
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5082..5098)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5082..5098)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5095..5117)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5106..5122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5130..5136,5137..5154,5155..5160))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5175..5196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5313..5330)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5484..6072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5564..5583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6243..7034,7035..7103))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6866..6885
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7104..7208)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7283..7302)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7474..7853
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7855..8053
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8071..8251
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8298..8423
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8916..9149
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9334..9378
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
       61 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
      121 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      181 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      241 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      301 acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa
      361 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      421 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
      481 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      541 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      601 agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg
      661 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
      721 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt
      781 catgtccccg tgtcctcccc agcagagcag gaacagggtg atacagctgt ccacttcaga
      841 gctaggagag atggaactga cttggcagga gatcatgtcc atcaccgagc tgcagggtct
      901 gaatgctcca agtgagccat catttgagcc ccaagcccca gctccatacc ttggacctcc
      961 accacccaca acttactgcc cctgctcaat ccacccagat tctggcttcc cacttcctcc
     1021 accaccttat gagctcccag catccacatc ccatgtccca gatcccccat actcctatgg
     1081 caacatggcc ataccagtct ccaagccact gagcctctca ggcctgctca gtgagccgct
     1141 ccaagacccc ttagccctcc tggacattgg gctgccagca gggccaccta agccccaaga
     1201 agacccagaa tccgactcag gattatccct caactatagc gatgctgaat ctcttgagct
     1261 ggaggggaca gaggctggtc ggcggcgcag cgaatatgta gagatgtacc cagtggagta
     1321 cccctactca ctcatgccca actccttggc ccactccaac tataccttgc cagctgctga
     1381 gacccccttg gccttagagc cctcctcagg ccctgtgcgg gctaagccca ctgcacgggg
     1441 ggaggcaggg agtcgggatg aacgtcgggc cttggccatg aagattcctt ttcctacgga
     1501 caagattgtc aacttgccgg tagatgactt taatgagcta ttggcaaggt acccgctgac
     1561 agagagccag ctagcgctag tccgggacat ccgacgacgg ggcaaaaaca aggtggcagc
     1621 ccagaactgc cgcaagagga agctggaaac cattgtgcag ctggagcggg agctggagcg
     1681 gctgaccaat gaacgggagc ggcttctcag ggcccgcggg gaggcagacc ggaccctgga
     1741 ggtcatgcgc caacagctga cagagctgta ccgtgacatt ttccagcacc ttcgggatga
     1801 atcaggcaac agctactctc ctgaagagta cgcgctgcaa caggctgccg atgggaccat
     1861 cttccttgtg ccccggggga ccaagatgga ggccacagac tgagaattcg atatcaagct
     1921 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1981 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2041 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2101 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2161 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2221 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2281 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2341 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2401 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2461 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     2521 ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     2581 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     2641 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     2701 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     2761 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     2821 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     2881 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     2941 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     3001 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     3061 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     3121 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     3181 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     3241 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     3301 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     3361 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3421 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3481 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     3541 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3601 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3661 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3721 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3781 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3841 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3901 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     3961 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4021 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4081 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4141 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4201 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     4261 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     4321 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     4381 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     4441 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     4501 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     4561 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     4621 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     4681 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     4741 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     4801 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     4861 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     4921 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4981 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5041 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     5101 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5161 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5221 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5281 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5341 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5401 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5461 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5521 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5581 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5641 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5701 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5761 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5821 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5881 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5941 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6001 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6061 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6121 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6181 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6241 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6301 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6361 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6421 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6481 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6541 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6601 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6661 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6721 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6781 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6841 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6901 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6961 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7021 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7081 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7141 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7201 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     7261 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     7321 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     7381 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     7441 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     7501 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     7561 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     7621 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     7681 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     7741 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     7801 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     7861 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     7921 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     7981 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     8041 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     8101 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8161 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8221 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     8281 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     8341 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8401 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8461 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8521 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8581 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8641 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8701 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8761 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     8821 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     8881 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     8941 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     9001 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     9061 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     9121 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     9181 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     9241 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     9301 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     9361 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     9421 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     9481 atagtgaata gagttaggca gggatattc
//
