LOCUS       sequence-291987-        8858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox inducible expression of luciferase + crIFNg. Constitutive EF1a 
            driven rtTA and zeocin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Kempton HR, Goudy LE, Love KS, Qi LS
  TITLE     Multiple Input Sensing and Signal Integration Using a Split Cas12a 
            System.
  JOURNAL   Mol Cell. 2020 Jan 30. pii: S1097-2765(20)30037-X. doi: 
            10.1016/j.molcel.2020.01.016.
  PUBMED    32027839
REFERENCE   2  (bases 1 to 8858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2020 Jan 30. pii: S1097-2765(20)30037-X. doi: 
            10.1016/j.molcel.2020.01.016."
COMMENT     Sequence Label: pSLQ8611
            PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-...
FEATURES             Location/Qualifiers
     source          1..8858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   38..72
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    391..409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    427..445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    463..481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    499..517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    535..553
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    571..589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     670..694
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             771..2423
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(837..855)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(2621..2640)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2675..2694
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    2767..3356
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2873..2884
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3283..3303
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3418..4164)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(4177..4230)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(4240..4611)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     intron          4648..5570
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4671..4691)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     repeat_region   5925..5987
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6034..6054)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6034..6052)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6073..6089)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6073..6089)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6086..6108)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6097..6113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6121..6127,6128..6145,6146..6151))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6166..6187
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6304..6321)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6475..7063)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6555..6574)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7234..8025,8026..8094))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7857..7876
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8095..8199)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8225..8680)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8362..8383)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8574..8593
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8807..8829
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8821..8838
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8822..8838
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(8848..8858,1..9)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(8848..8858,1..8)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actatagggc gaattggggc gcgccattct agattaaccc tagaaagata gtctgcgtaa
       61 aattgacgca tgcattcttg aaatattgct ctctctttct aaatagcgcg aatccgtcgc
      121 tgtgcattta ggacatctca gtcgccgctt ggagctcccg tgaggcgtgc ttgtcaatgc
      181 ggtaagtgtc actgattttg aactataacg accgcgtgag tcaaaatgac gcatgattat
      241 cttttacgtg acttttaaga tttaactcat acgataatta tattgttatt tcatgttcta
      301 cttacgtgat aacttattat atatatattt tcttgttata gatatcaact agaatgctag
      361 cgctgatatc tccaccccac agtggtttac tccctatcag tgatagagaa cgtatgaaga
      421 gtttactccc tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag
      481 aacgtataag gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta
      541 tcagtgatag agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca
      601 gtttactccc tatcagtgat agagaacgta taagctttag gcgtgtacgg tgggcgccta
      661 taaaagcaga gctcgtttag tgaaccgtca gatcgcctgg agcaattcca caacactttt
      721 gtcttatacc aactttccgt accacttcct accctcgtaa agaattcacc atggaagacg
      781 ccaaaaacat aaagaaaggc ccggcgccat tctatccact agaggatgga accgctggag
      841 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
      901 atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg
      961 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
     1021 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
     1081 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta
     1141 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac
     1201 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
     1261 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag
     1321 agtcctttga tcgtgacaaa acaattgcac tgataatgaa ctcctctgga tctactgggt
     1381 tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag
     1441 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
     1501 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
     1561 taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa
     1621 gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat
     1681 acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg
     1741 gggaggcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg
     1801 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta
     1861 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg
     1921 ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt tatgtaaaca
     1981 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag
     2041 cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat
     2101 acaaaggata ccaggtggcc cccgctgaat tggagtcgat attgttacaa caccccaaca
     2161 tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg
     2221 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca
     2281 gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga
     2341 aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga
     2401 agggcggaaa gtccaaattg taaggatcct ttctttcttt gaatttctac taagtgtaga
     2461 tagatgagat ggtgacagat aggcaatttc tactaagtgt agatagatga gatggtgaca
     2521 gataggcaat ttctactaag tgtagatcga gttttctttt tgttttgtac aagtaagtta
     2581 cgcgtcttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2641 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2701 tcttatcatg tctggatcaa ctggataact caagctaacc gtcatacata tgtcgacggt
     2761 atcgatgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac
     2821 gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg
     2881 ccgaagggac gtagcagaag gacgtccccg cgcagaatcc aggtggcaac acaggcgagc
     2941 agccaaggaa aggacgatga tttccccgac aacaccacgg aattgtcagt gcccaacagc
     3001 cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg
     3061 agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg
     3121 ggggttgcgt cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac
     3181 tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg
     3241 gaagcaatag catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca
     3301 acatagttaa gaataccagt caatctttca caaattttgt aatccagagg ttgattatcg
     3361 ataagcttga tatcaagctt gcatgcctgc aggtcgactc tagagtctta tgcggcctta
     3421 gcctggcagc atatcgagat cgaagtcatc gagagcgtca gcagggagca tatccagatc
     3481 aaaatcatcc agggcatcgg cgggcagcat gtccaggtcg aagtcgtcca gggcgtcggt
     3541 gggtccgccg ctctcgcact tcagctgctt ctcgaggccg cagatgatca gttccaggcc
     3601 gaacaggaag gcgggctcgg cgccctgtct gtcgaacagc tcgatggcct gcttcagcag
     3661 ggggggcatg ctgtcggtgg tgggtgtctc tctttcctct ttggcgacct ggtgttcctg
     3721 ttcttccagc acgcagccca gggtgaagtg gcccacggcg ctcagggcgt acagggcgtt
     3781 ttccaggctg aagccctgct ggcacaggaa ggccagctgg ttttccagtg tctcgtactg
     3841 cttctcggtg ggtctggtgc ccaggtgcac tttggcgccg tcccggtggc tcagcagggc
     3901 gcatctgtag ctcttggcgt tgttccgcag gaagtcctgc cagctctcgc cttccagagg
     3961 gcagctgtgg gtgtggtgcc ggtccagcat ctcgatgggc agggcgtcca gcagggcccg
     4021 cttgttcttc acgtgccagt acagggtggg ctgttccacg cccagtttct gggccagctt
     4081 ccgggtggtc aggccctcga tgcccacgcc gttcagcagt tccagggcgc tgttgatcac
     4141 tttgctcttg tccagccggc tcatcgaccc tccgcctggg ccaggattct cctcgacgtc
     4201 accgcatgtt agcagacttc ctctgccctc ggaaccgccg tcctgctcct cggccacgaa
     4261 gtgcacgcag ttgccggccg ggtcgcgcag ggcgaactcc cgcccccacg gctgctcgcc
     4321 gatctcggtc atggccggcc cggaggcgtc ccggaagttc gtggacacga cctccgacca
     4381 ctcggcgtac agctcgtcca ggccgcgcac ccacacccag gccagggtgt tgtccggcac
     4441 cacctggtcc tggaccgcgc tgatgaacag ggtcacgtcg tcccggacca caccggcgaa
     4501 gtcgtcctcc acgaagtccc gggagaaccc gagccggtcg gtccagaact cgaccgctcc
     4561 ggcgacgtcg cgcgcggtga gcaccggaac ggcactggtc aacttggcca taattctcga
     4621 gagctccaga tcccctgttc acgacacctg aaatggaaga aaaaaacttt gaaccactgt
     4681 ctgaggcttg agaatgaacc aagatccaaa ctcaaaaagg ccaaattcca aggagaatta
     4741 catcaagtgc caagctggcc taacttcagt ctccacccac tcagtgtggg gaaactccat
     4801 cgcataaaac ccctcccccc aacctaaaga cgacgtactc caaaagctcc agaactaatc
     4861 gaggtgcctg gacggcgccc ggtactccgt ggagtcacat gaagcgacgg ctgaggacgg
     4921 aaaggccctt ttcctttgtg tgggtgactc acccgcccgc tctcccgagc gccgcgtcct
     4981 ccattttgag ccccctggag cagggccggg aagcggccat ctttccgctc acgcaactgg
     5041 tgccgaccgg gccagccttg ccgcccaggg cggggcgata cacggcggcg cgaggccagg
     5101 caccagagca ggccggccag cttgagacta cccccgtccg attctcggtg gccgcgctcg
     5161 caggccccgc ctcgccgaac atgtgcgctg ggacgcacgg gccccgtcgc cggccgcggg
     5221 cccaaaaacc gaaataccag tgtgcagatc ctggcccgca tttacaagac tatcttgcca
     5281 gaaaaaaagc gtcgcagcac gtcatcaaaa attttaaatg gctagagact tatcgaaagc
     5341 agcgagacag gcgcgaaggt gccaccagat tcgcacgcgg cggccccagc gcccaggcca
     5401 ggcctcaact caagcacgag gcgaaggggc tcctaaagcg caaggcccgc ccctggctcc
     5461 agctcgggat caagaatcac gtactggagc caggtggaag taattcaagg cacgcaaggg
     5521 ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg
     5581 gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct
     5641 cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg
     5701 cgccaccttc tctaggcacc ggttcaattg gctgcaggac tagtctagtt aaaagttttg
     5761 ttactttata gaagaaattt tgagtttttg ttttttttta ataaataaat aaacataaat
     5821 aaattgtttg ttgaatttat tattagtatg taagtgtaaa tataataaaa cttaatatct
     5881 attcaaatta ataaataaac ctcgatatac agaccgataa aacacatgcg tcaattttac
     5941 gcatgattat ctttaacgta cgtcacaata tgattatctt tctagggtta atctagtgtt
     6001 taaacggccg cttaattaat ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     6061 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6121 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6181 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6241 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6301 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6361 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6421 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6481 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6541 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6601 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6661 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6721 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6781 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6841 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6901 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6961 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7021 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7081 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7141 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7201 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7261 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7321 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7381 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7441 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7501 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7561 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7621 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7681 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7741 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7801 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7861 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7921 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7981 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8041 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8101 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8161 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8221 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     8281 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     8341 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     8401 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     8461 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     8521 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     8581 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     8641 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     8701 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     8761 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     8821 tgtaaaacga cggccagtga gcgcgcgtaa tacgactc
//
