LOCUS       sequence-291993-        9759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive PGK driven expression of full length dCpf1 (Cas12a) 
            -miniVPR activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9759)
  AUTHORS   Kempton HR, Goudy LE, Love KS, Qi LS
  TITLE     Multiple Input Sensing and Signal Integration Using a Split Cas12a 
            System.
  JOURNAL   Mol Cell. 2020 Jan 30. pii: S1097-2765(20)30037-X. doi: 
            10.1016/j.molcel.2020.01.016.
  PUBMED    32027839
REFERENCE   2  (bases 1 to 9759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2020 Jan 30. pii: S1097-2765(20)30037-X. doi: 
            10.1016/j.molcel.2020.01.016."
COMMENT     Sequence Label: pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA
FEATURES             Location/Qualifiers
     source          1..9759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..500
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(36..59)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     433..452
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             514..4197
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4204..4230
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4210..4236
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4249..4269
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4276..4296
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4333..4482
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4489..4509
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4642..4998
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             5233..5943
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5378..5396)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5646..5665
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(5962..5980)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5963..5980)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6000..6017)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6006..6230
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6446..6508
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6559..6579)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6559..6577)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6598..6614)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6598..6614)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6611..6633)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6622..6638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6646..6652,6653..6670,6671..6676))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6691..6712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6829..6846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7000..7588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7080..7099)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7759..8550,8551..8619))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8382..8401
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8620..8724)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8750..9205)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8887..8908)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9099..9118
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9332..9354
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9346..9363
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9347..9363
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9373..9392
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9373..9391
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   9421..9455
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
       61 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
      121 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
      181 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
      241 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
      301 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
      361 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
      421 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
      481 cctcatctcc gggcctttcg agaattcggt accatgagca agctggagaa gtttacaaac
      541 tgctactccc tgtctaagac cctgaggttc aaggccatcc ctgtgggcaa gacccaggag
      601 aacatcgaca ataagcggct gctggtggag gacgagaaga gagccgagga ttataagggc
      661 gtgaagaagc tgctggatcg ctactatctg tcttttatca acgacgtgct gcacagcatc
      721 aagctgaaga atctgaacaa ttacatcagc ctgttccgga agaaaaccag aaccgagaag
      781 gagaataagg agctggagaa cctggagatc aatctgcgga aggagatcgc caaggccttc
      841 aagggcaacg agggctacaa gtccctgttt aagaaggata tcatcgagac aatcctgcca
      901 gagttcctgg acgataagga cgagatcgcc ctggtgaaca gcttcaatgg ctttaccaca
      961 gccttcaccg gcttctttga taacagagag aatatgtttt ccgaggaggc caagagcaca
     1021 tccatcgcct tcaggtgtat caacgagaat ctgacccgct acatctctaa tatggacatc
     1081 ttcgagaagg tggacgccat ctttgataag cacgaggtgc aggagatcaa ggagaagatc
     1141 ctgaacagcg actatgatgt ggaggatttc tttgagggcg agttctttaa ctttgtgctg
     1201 acacaggagg gcatcgacgt gtataacgcc atcatcggcg gcttcgtgac cgagagcggc
     1261 gagaagatca agggcctgaa cgagtacatc aacctgtata atcagaaaac caagcagaag
     1321 ctgcctaagt ttaagccact gtataagcag gtgctgagcg atcgggagtc tctgagcttc
     1381 tacggcgagg gctatacatc cgatgaggag gtgctggagg tgtttagaaa caccctgaac
     1441 aagaacagcg agatcttcag ctccatcaag aagctggaga agctgttcaa gaattttgac
     1501 gagtactcta gcgccggcat ctttgtgaag aacggccccg ccatcagcac aatctccaag
     1561 gatatcttcg gcgagtggaa cgtgatccgg gacaagtgga atgccgagta tgacgatatc
     1621 cacctgaaga agaaggccgt ggtgaccgag aagtacgagg acgatcggag aaagtccttc
     1681 aagaagatcg gctccttttc tctggagcag ctgcaggagt acgccgacgc cgatctgtct
     1741 gtggtggaga agctgaagga gatcatcatc cagaaggtgg atgagatcta caaggtgtat
     1801 ggctcctctg agaagctgtt cgacgccgat tttgtgctgg agaagagcct gaagaagaac
     1861 gacgccgtgg tggccatcat gaaggacctg ctggattctg tgaagagctt cgagaattac
     1921 atcaaggcct tctttggcga gggcaaggag acaaacaggg acgagtcctt ctatggcgat
     1981 tttgtgctgg cctacgacat cctgctgaag gtggaccaca tctacgatgc catccgcaat
     2041 tatgtgaccc agaagcccta ctctaaggat aagttcaagc tgtattttca gaaccctcag
     2101 ttcatgggcg gctgggacaa ggataaggag acagactatc gggccaccat cctgagatac
     2161 ggctccaagt actatctggc catcatggat aagaagtacg ccaagtgcct gcagaagatc
     2221 gacaaggacg atgtgaacgg caattacgag aagatcaact ataagctgct gcccggccct
     2281 aataagatgc tgccaaaggt gttcttttct aagaagtgga tggcctacta taaccccagc
     2341 gaggacatcc agaagatcta caagaatggc acattcaaga agggcgatat gtttaacctg
     2401 aatgactgtc acaagctgat cgacttcttt aaggatagca tctcccggta tccaaagtgg
     2461 tccaatgcct acgatttcaa cttttctgag acagagaagt ataaggacat cgccggcttt
     2521 tacagagagg tggaggagca gggctataag gtgagcttcg agtctgccag caagaaggag
     2581 gtggataagc tggtggagga gggcaagctg tatatgttcc agatctataa caaggacttt
     2641 tccgataagt ctcacggcac acccaatctg cacaccatgt acttcaagct gctgtttgac
     2701 gagaacaatc acggacagat caggctgagc ggaggagcag agctgttcat gaggcgcgcc
     2761 tccctgaaga aggaggagct ggtggtgcac ccagccaact cccctatcgc caacaagaat
     2821 ccagataatc ccaagaaaac cacaaccctg tcctacgacg tgtataagga taagaggttt
     2881 tctgaggacc agtacgagct gcacatccca atcgccatca ataagtgccc caagaacatc
     2941 ttcaagatca atacagaggt gcgcgtgctg ctgaagcacg acgataaccc ctatgtgatc
     3001 ggcatcgcca ggggcgagcg caatctgctg tatatcgtgg tggtggacgg caagggcaac
     3061 atcgtggagc agtattccct gaacgagatc atcaacaact tcaacggcat caggatcaag
     3121 acagattacc actctctgct ggacaagaag gagaaggaga ggttcgaggc ccgccagaac
     3181 tggacctcca tcgagaatat caaggagctg aaggccggct atatctctca ggtggtgcac
     3241 aagatctgcg agctggtgga gaagtacgat gccgtgatcg ccctggagga cctgaactct
     3301 ggctttaaga atagccgcgt gaaggtggag aagcaggtgt atcagaagtt cgagaagatg
     3361 ctgatcgata agctgaacta catggtggac aagaagtcta atccttgtgc aacaggcggc
     3421 gccctgaagg gctatcagat caccaataag ttcgagagct ttaagtccat gtctacccag
     3481 aacggcttca tcttttacat ccctgcctgg ctgacatcca agatcgatcc atctaccggc
     3541 tttgtgaacc tgctgaaaac caagtatacc agcatcgccg attccaagaa gttcatcagc
     3601 tcctttgaca ggatcatgta cgtgcccgag gaggatctgt tcgagtttgc cctggactat
     3661 aagaacttct ctcgcacaga cgccgattac atcaagaagt ggaagctgta ctcctacggc
     3721 aaccggatca gaatcttccg gaatcctaag aagaacaacg tgttcgactg ggaggaggtg
     3781 tgcctgacca gcgcctataa ggagctgttc aacaagtacg gcatcaatta tcagcagggc
     3841 gatatcagag ccctgctgtg cgagcagtcc gacaaggcct tctactctag ctttatggcc
     3901 ctgatgagcc tgatgctgca gatgcggaac agcatcacag gccgcaccga cgtggatttt
     3961 ctgatcagcc ctgtgaagaa ctccgacggc atcttctacg atagccggaa ctatgaggcc
     4021 caggagaatg ccatcctgcc aaagaacgcc gacgccaatg gcgcctataa catcgccaga
     4081 aaggtgctgt gggccatcgg ccagttcaag aaggccgagg acgagaagct ggataaggtg
     4141 aagatcgcca tctctaacaa ggagtggctg gagtacgccc agaccagcgt gaagcacgga
     4201 tcctatccct atgacgtgcc cgattatgcc agcctgggca gcggctcccc caagaaaaaa
     4261 cgcaaggtgg aagatcctaa gaaaaagcgg aaagtggacg gcattggtag tgggagcaac
     4321 ggcagcagcc tcgatgcttt agacgatttt gacttagata tgcttggttc agacgcgtta
     4381 gacgacttcg acctagacat gttaggctca gatgcattgg acgacttcga tttagatatg
     4441 ttgggctccg atgccctaga tgactttgat ctagatatgc taggtagtcc caaaaagaag
     4501 aggaaagtgg gatccgtcct tccgcaagcg cctgccccag cgcctgcacc ggcaatggtg
     4561 tccgccctcg cacaggcccc tgcgcccgtc cccgtgctcg cgcctggacc gccccaggcg
     4621 gtcgctccac cggctccgaa gccgacgcag gccggagagg gaacactctc cgaagcactt
     4681 cttcaactcc agtttgatga cgaggatctt ggagcactcc ttggaaactc gacagaccct
     4741 gcggtgttta ccgacctcgc gtcagtagat aactccgaat ttcagcagct tttgaaccag
     4801 ggtatcccgg tcgcgccaca tacaacggag cccatgttga tggaataccc cgaagcaatc
     4861 acgagacttg tgacgggagc gcagcggcct cccgatcccg cacccgcacc tttgggggca
     4921 cctggcctcc ctaacggact tttgagcggc gacgaggatt tctcctccat cgccgatatg
     4981 gatttctcag ccttgctgtc aggctctggc agcggcagcg acctttccca tccgccccca
     5041 aggggccatc tggatgagct gacaaccaca cttgagtcca tgaccgagga tctgaacctg
     5101 gactcacccc tgaccccgga attgaacgag attctggata ccttcctgaa cgacgagtgc
     5161 ctcttgcatg ccatgcatat cagcacagga ctgtccatct tcgacacatc tctgtttggc
     5221 agcagcggat ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     5281 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     5341 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     5401 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     5461 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     5521 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     5581 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     5641 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     5701 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     5761 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     5821 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     5881 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     5941 taactcgaga gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc
     6001 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     6061 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     6121 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     6181 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggt
     6241 cgaggcggcc gctgatcaca attgtctagt taaaagtttt gttactttat agaagaaatt
     6301 ttgagttttt gttttttttt aataaataaa taaacataaa taaattgttt gttgaattta
     6361 ttattagtat gtaagtgtaa atataataaa acttaatatc tattcaaatt aataaataaa
     6421 cctcgatata cagaccgata aaacacatgc gtcaatttta cgcatgatta tctttaacgt
     6481 acgtcacaat atgattatct ttctagggtt aatctagtat acgcgtatgc ggccgcttaa
     6541 ttaatccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     6601 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6661 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6721 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6781 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6841 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6901 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6961 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7021 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7081 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7141 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7201 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7261 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7321 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7381 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7441 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7501 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7561 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7621 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7681 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7741 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7801 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7861 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7921 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7981 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8041 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8101 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8161 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8221 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8281 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8341 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8401 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8461 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8521 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8581 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8641 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8701 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     8761 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     8821 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     8881 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     8941 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     9001 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     9061 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     9121 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     9181 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     9241 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     9301 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     9361 agtgagcgcg cgtaatacga ctcactatag ggcgaattgg ggcgcgccat tctagattaa
     9421 ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt
     9481 tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc
     9541 ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt
     9601 gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact catacgataa
     9661 ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt
     9721 atagatatca actagaatgc tagcatgggc ccagatctc
//
