LOCUS       sequence-291994-       11072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible, wt C-terminal His tagged RPB1, resistant to siRNAs 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11072)
  AUTHORS   Tufegdzic Vidakovic A, Mitter R, Kelly GP, Neumann M, Harreman M, 
            Rodriguez-Martinez M, Herlihy A, Weems JC, Boeing S, Encheva V, Gaul
            L, Milligan L, Tollervey D, Conaway RC, Conaway JW, Snijders AP, 
            Stewart A, Svejstrup JQ
  TITLE     Regulation of the RNAPII Pool Is Integral to the DNA Damage 
            Response.
  JOURNAL   Cell. 2020 Mar 5. pii: S0092-8674(20)30153-7. doi: 
            10.1016/j.cell.2020.02.009.
  PUBMED    32142654
REFERENCE   2  (bases 1 to 11072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Mar 5. pii: S0092-8674(20)30153-7. doi: 10.1016/j.cell.2020.02.009."
COMMENT     Sequence Label: pFRT-TO-RPB1-His WT CRres si2,4R
FEATURES             Location/Qualifiers
     source          1..11072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1592..1601
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1995..2004
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6966..6983
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7024..7041)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7030..7254
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7387..7406)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7538..7585
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7593..8615
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7593..8615
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8745..8866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8782..8801)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8836..8855
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8915..8931)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8915..8931)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8928..8950)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8939..8955
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8963..8969,8970..8987,8988..8993))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9008..9029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9146..9163)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9317..9905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9397..9416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10076..10867,10868..10936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10699..10718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10937..11041)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     1021 attctgcaga tatccagcac agtggcggcc gcatgcacgg gggtggcccc ccctcggggg
     1081 acagcgcatg cccgctgcgc accatcaaga gagtccagtt cggagtcctg agtccggatg
     1141 aactgaagcg aatgtctgtg acggagggtg gcatcaaata cccagagacg actgagggag
     1201 gccgccccaa gcttgggggg ctgatggacc cgaggcaggg ggtgattgag cggactggcc
     1261 gctgccaaac atgtgcagga aacatgacag agtgtcctgg ccactttggc cacattgaac
     1321 tggccaagcc tgtgtttcac gtgggcttcc tggtgaagac aatgaaagtt ttgcgctgtg
     1381 tctgcttctt ctgctccaaa ctgcttgtgg actctaacaa cccaaagatc aaggatatcc
     1441 tggctaagtc caagggacag cccaagaagc ggctcacaca tgtctacgac ctttgcaagg
     1501 gcaaaaacat atgcgagggt ggggaggaga tggacaacaa gttcggtgtg gaacaacctg
     1561 agggtgacga ggatctgacc aaagaaaagg gccatggtgg ctgtgggcgg taccagccca
     1621 ggatccggcg ttctggccta gagctgtatg cggaatggaa gcacgttaat gaggactctc
     1681 aggagaagaa gatcctgctg agtccagagc gagtgcatga gatcttcaaa cgcatctcag
     1741 atgaggagtg ttttgtgctg ggcatggagc cccgctatgc acggccagag tggatgattg
     1801 tcacagtgct gcctgtgccc ccgctctccg tgcggcctgc tgttgtgatg cagggctctg
     1861 cccgtaacca ggatgacctg actcacaaac tggctgacat cgtgaagatc aacaatcagc
     1921 tgcggcgcaa tgagcagaac ggcgcagcgg cccatgtcat tgcagaggat gtgaagctcc
     1981 tccagttcca tgtggccacc atggtggaca atgagctgcc tggcttgccc cgtgccatgc
     2041 agaagtctgg gcgtcccctc aagtccctga agcagcggtt gaagggcaag gaaggccggg
     2101 tgcgagggaa cctgatgggc aaaagagtgg acttctcggc ccgtactgtc atcacccccg
     2161 accccaacct ctccattgac caggttggcg tgccccgctc cattgctgcc aacatgacct
     2221 ttgcggagat tgtcaccccc ttcaacattg acagacttca agaactagtg cgcaggggga
     2281 acagccagta cccaggcgcc aagtacatca tccgagacaa tggtgatcgc attgacttgc
     2341 gtttccaccc caagcccagt gaccttcacc tgcagaccgg ctataaggtg gaacggcaca
     2401 tgtgtgatgg ggacattgtt atcttcaacc ggcagccaac tctgcacaaa atgtccatga
     2461 tggggcatcg ggtccgcatt ctcccatggt ctacctttcg cttgaatctt agtgtgacaa
     2521 ctccgtacaa tgcagacttt gacggtgacg aaatgaatct tcacctgcca cagtctctgg
     2581 agacgcgagc agagatccag gagctggcca tggttcctcg catgattgtc accccccaga
     2641 gcaatcggcc tgtcatgggt attgtgcagg acacactcac agcagtgcgc aaattcacca
     2701 agagagacgt cttcctggag cggggtgaag tgatgaacct cctgatgttc ctgtcgacgt
     2761 gggatgggaa ggtcccacag ccggccatcc taaagccccg gcccctgtgg acaggcaagc
     2821 aaatcttctc cctcatcata cctggtcaca tcaattgtat ccgtacccac agcacccatc
     2881 ccgatgatga agacagtggc ccttacaagc acatctctcc tggggacacc aaggtggtgg
     2941 tggagaatgg ggagctgatc atgggcatcc tgtgtaagaa gtctctgggc acgtcagctg
     3001 gctccctggt ccacatctcc tacctagaga tgggtcatga catcactcgc ctcttctact
     3061 ccaacattca gactgtcatt aacaactggc tcctcatcga gggtcatact attggcattg
     3121 gggactccat tgctgattct aagacttacc aggacattca gaacactatt aagaaggcca
     3181 agcaggacgt tatcgaagtg attgaaaagg cacacaacaa tgagctggag cccaccccag
     3241 ggaacactct gcggcagacg tttgagaatc aggtgaaccg cattcttaac gatgcccgag
     3301 acaagactgg ctcctctgct cagaaatccc tgtctgaata caacaacttc aagtctatgg
     3361 tcgtgtccgg agctaaaggt tccaagatta acatctccca ggtcattgct gtcgttggac
     3421 agcagaacgt cgagggcaag cggattccat ttggcttcaa gcaccggact ctgcctcact
     3481 tcatcaagga tgactacggg cctgagagcc gtggctttgt ggagaactcc tacctagccg
     3541 gcctcacacc cactgagttc tttttccacg ccatgggggg tcgtgagggg ctcattgaca
     3601 cggctgtcaa gactgctgag actggataca tccagcggcg gctgatcaag tccatggagt
     3661 cagtgatggt gaagtacgac gcgactgtgc ggaactccat caaccaggtg gtgcagctgc
     3721 gctacggcga agacggcctg gcaggcgaga gcgttgagtt ccagaacctg gctacgctta
     3781 agccttccaa caaggctttt gagaagaagt tccgctttga ttataccaat gagagggccc
     3841 tgcggcgcac tctgcaggag gacctggtga aggacgtgct gagcaacgca cacatccaga
     3901 acgagttgga gcgggaattt gagcggatgc gggaggatcg ggaggtgctc agggtcatct
     3961 tcccaactgg agacagcaag gtcgtcctcc cctgtaacct gctgcggatg atctggaatg
     4021 ctcagaaaat cttccacatc aacccacgcc ttccctccga cctgcacccc atcaaagtgg
     4081 tggagggagt caaggaattg agcaagaagc tggtgattgt gaatggggat gacccactaa
     4141 gtcgacaggc ccaggaaaat gccacgctgc tcttcaacat ccacctgcgg tccacgttgt
     4201 gttcccgccg catggcagag gagtttcggc tcagtgggga ggccttcgac tggctgcttg
     4261 gggagattga gtccaagttc aaccaagcca ttgcgcatcc cggggaaatg gtgggggctc
     4321 tggctgcgca gtcccttgga gaacctgcca cccagatgac cttgaatacc ttccactatg
     4381 ctggtgtgtc tgccaagaat gtgacgctgg gtgtgccccg acttaaggag ctcatcaaca
     4441 tttccaagaa gccaaagact ccttcgctta ctgtcttcct gttgggccag tccgctcgtg
     4501 atgctgagag agccaaggat attctgtgcc gtctggagca tacaacgttg aggaaggtga
     4561 ctgccaacac agccatctac tatgacccca acccccagag cacggtggtg gcagaggatc
     4621 aggaatgggt gaatgtctac tatgaaatgc ctgactttga tgtggcccga atctccccct
     4681 ggctgttgcg ggtggagctg gatcggaagc acatgactga ccggaagctc accatggagc
     4741 agattgctga aaagatcaat gctggttttg gtgacgactt gaactgcatc tttaatgatg
     4801 acaatgcaga gaagctggtg ctccgtattc gcatcatgaa cagtgatgag aacaagatgc
     4861 aagaagagga agaggtggtg gacaagatgg atgatgatgt cttcctgcgc tgcatcgagt
     4921 ccaacatgct gacagatatg accctgcagg gcatcgagca gatcagcaag gtgtacatgc
     4981 acttgccaca gacagacaac aagaagaaga tcatcatcac ggaggatggg gaattcaagg
     5041 ccctgcagga gtggatcctg gagacggacg gcgtgagctt gatgcgggtg ctgtcagaga
     5101 aagacgtgga tccagtacgc acaacgtcga atgacattgt ggagatcttc acggtgctgg
     5161 gcattgaagc cgtgcggaag gccctggagc gggagctgta ccacgtcatc tcctttgatg
     5221 gctcctatgt caattaccga cacttggctc tcttgtgtga taccatgacc tgtcgtggcc
     5281 acttgatggc catcacccga cacggagtca accgccagga cacaggacca ctcatgaagt
     5341 gttcctttga ggaaacggtg gacgtgctta tggaagcagc cgcacacggt gagagtgacc
     5401 ccatgaaggg ggtctctgag aatatcatgc tgggccagct ggctccggcc ggcactggct
     5461 gctttgacct cctgcttgat gcagagaagt gcaagtatgg catggagatc cccaccaata
     5521 tccccggcct gggggctgct ggacccaccg gcatgttctt tggttcagca cccagtccca
     5581 tgggtggaat ctctcctgcc atgacacctt ggaaccaggg tgcaacccct gcctatggcg
     5641 cctggtcccc cagtgttggg agtggaatga ccccaggggc agccggcttc tctcccagtg
     5701 ctgcgtcaga tgccagcggc ttcagcccag gttactcccc tgcctggtct cccacaccgg
     5761 gctccccggg gtccccaggt ccctcaagcc cctacatccc ttcaccaggt ggtgccatgt
     5821 ctcccagcta ctcgccaacg tcacctgcct acgagccccg ctctcctggg ggctacacac
     5881 cccagagtcc ctcttattcc cccacttcac cctcctactc ccctacctct ccatcctatt
     5941 ctccaaccag tcccaactat agtcccacat cacccagcta ttcgccaacg tcacccagct
     6001 actcaccgac ctctcccagc tactcaccca cctctcccag ctactcgccc acctctccca
     6061 gctactcgcc cacctctccc agctactcac ccacttcccc tagctactcg cccacttccc
     6121 ctagctactc gccaacgtct cccagctact cgccgacatc tcccagctac tcgccaactt
     6181 cacccagcta ttctcccact tctcccagct actcacctac ctctccaagc tattcaccca
     6241 cctcccccag ctactcaccc acttccccaa gttactcacc caccagcccg aactattctc
     6301 caaccagtcc caattacacc ccaacatcac ccagctacag cccgacatca cccagctatt
     6361 cacctactag tcccaactac acacctacca gccctaacta cagcccaacc tctccaagct
     6421 actctccaac atcacccagc tattccccga cctcaccaag ttactcccct tccagcccac
     6481 gatacacacc acagtctcca acctataccc caagctcacc cagctacagc cccagctcgc
     6541 ccagctacag cccaacctca cccaagtaca ccccaaccag tccttcttac agtcccagct
     6601 ccccagagta taccccaacc tctcccaagt actcacctac cagtcccaaa tattcaccca
     6661 cctctcccaa gtactcgcct accagtccca cctattcacc caccacccca aaatactccc
     6721 caacatctcc tacttattcc ccaacctctc cagtctacac cccaacctct cccaagtact
     6781 cacctactag ccccacttac tcgcccactt cccccaagta ctcgcccacc agccccacct
     6841 actcgcccac ctcccccaaa ggctcaacct actctcccac ttcccctggt tactcgccca
     6901 ccagccccac ctacagtctc acaagcccgg ctatcagccc ggatgacagt gacgaggaga
     6961 acgctcacca tcaccatcac cattgactcg agtctagagg gcccgtttaa acccgctgat
     7021 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     7081 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     7141 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     7201 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     7261 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     7321 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     7381 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     7441 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     7501 ccaaaaaact tgattagggt gatggttcac gtacctagaa gttcctattc cgaagttcct
     7561 attctctaga aagtatagga acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg
     7621 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     7681 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     7741 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     7801 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     7861 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     7921 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     7981 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     8041 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     8101 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     8161 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     8221 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     8281 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     8341 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     8401 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     8461 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     8521 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     8581 gacgccccag cactcgtccg agggcaaagg aatagcacgt actacgagat ttcgattcca
     8641 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     8701 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     8761 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8821 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     8881 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     8941 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     9001 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9061 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     9121 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9481 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9721 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     9781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9961 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    10021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    10081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    10141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    10201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    10261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    10321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    10441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    10501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    10561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    10621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    10681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    11041 gcacatttcc ccgaaaagtg ccacctgacg tc
//
