LOCUS       sequence-291996-       13827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hypoxia-inducible expression of leucine zipper with C terminal dCpf1
            (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven 
            puromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13827)
  AUTHORS   Kempton HR, Goudy LE, Love KS, Qi LS
  TITLE     Multiple Input Sensing and Signal Integration Using a Split Cas12a 
            System.
  JOURNAL   Mol Cell. 2020 Jan 30. pii: S1097-2765(20)30037-X. doi: 
            10.1016/j.molcel.2020.01.016.
  PUBMED    32027839
REFERENCE   2  (bases 1 to 13827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2020 Jan 30. pii: S1097-2765(20)30037-X. doi: 
            10.1016/j.molcel.2020.01.016."
COMMENT     Sequence Label: pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406)...
FEATURES             Location/Qualifiers
     source          1..13827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3012..3038
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3018..3044
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3057..3077
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3084..3104
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3141..3290
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             3297..3317
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3450..3806
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             4041..4751
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4186..4204)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4454..4473
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        4758..4969
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4980..5579
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4980..4999)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5476..5496
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5580..6168
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5633..5653)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6051..6062)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6281..6514
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6539..6557)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6540..6557)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6847..6866)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7080..7099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7199..7221
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7259..7277)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7345..7449
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7450..7518,7519..8310)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7668..7687)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8481..9069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8970..8989
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9223..9240
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9310..9330)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9315..9644
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9495..9630
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10778..10843
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10973..10993
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11418..11552
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11455..11474)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11509..11528
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11720..12353
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12400..12525
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13022..13255
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13440..13484
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(13769..13827,1..59)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
       61 gggtttatta cagggacagc agagatccag tttatcgatg aggccctttc gtcttcactc
      121 gagcggatcc gaccttgagt acgtgcgtct ctgcacgtat ggcgattaag accttgagta
      181 cgtgcgtctc tgcacgtatg gcgattaaga ccttgagtac gtgcgtctct gcacgtatgg
      241 cgattaagac cttgagtacg tgcgtctctg cacgtatggc gattaatcat atgctctaga
      301 gggtatataa tgggggccac tagtctacta ccagaaagct tacgcgtacg accatgctgg
      361 agatccgcgc cgccttcctg cgccagcgca acaccgccct gcgcaccgag gtggccgagc
      421 tggagcagga ggtgcagcgc ctggagaacg aggtgagcca gtacgagacc cgctacggcc
      481 ccctgggcgg cggcaaggct agcggaggag gtggtggagg tggaggtgga ggtcatatgg
      541 atctgtctgt ggtggagaag ctgaaggaga tcatcatcca gaaggtggat gagatctaca
      601 aggtgtatgg ctcctctgag aagctgttcg acgccgattt tgtgctggag aagagcctga
      661 agaagaacga cgccgtggtg gccatcatga aggacctgct ggattctgtg aagagcttcg
      721 agaattacat caaggccttc tttggcgagg gcaaggagac aaacagggac gagtccttct
      781 atggcgattt tgtgctggcc tacgacatcc tgctgaaggt ggaccacatc tacgatgcca
      841 tccgcaatta tgtgacccag aagccctact ctaaggataa gttcaagctg tattttcaga
      901 accctcagtt catgggcggc tgggacaagg ataaggagac agactatcgg gccaccatcc
      961 tgagatacgg ctccaagtac tatctggcca tcatggataa gaagtacgcc aagtgcctgc
     1021 agaagatcga caaggacgat gtgaacggca attacgagaa gatcaactat aagctgctgc
     1081 ccggccctaa taagatgctg ccaaaggtgt tcttttctaa gaagtggatg gcctactata
     1141 accccagcga ggacatccag aagatctaca agaatggcac attcaagaag ggcgatatgt
     1201 ttaacctgaa tgactgtcac aagctgatcg acttctttaa ggatagcatc tcccggtatc
     1261 caaagtggtc caatgcctac gatttcaact tttctgagac agagaagtat aaggacatcg
     1321 ccggctttta cagagaggtg gaggagcagg gctataaggt gagcttcgag tctgccagca
     1381 agaaggaggt ggataagctg gtggaggagg gcaagctgta tatgttccag atctataaca
     1441 aggacttttc cgataagtct cacggcacac ccaatctgca caccatgtac ttcaagctgc
     1501 tgtttgacga gaacaatcac ggacagatca ggctgagcgg aggagcagag ctgttcatga
     1561 ggcgcgcctc cctgaagaag gaggagctgg tggtgcaccc agccaactcc cctatcgcca
     1621 acaagaatcc agataatccc aagaaaacca caaccctgtc ctacgacgtg tataaggata
     1681 agaggttttc tgaggaccag tacgagctgc acatcccaat cgccatcaat aagtgcccca
     1741 agaacatctt caagatcaat acagaggtgc gcgtgctgct gaagcacgac gataacccct
     1801 atgtgatcgg catcgccagg ggcgagcgca atctgctgta tatcgtggtg gtggacggca
     1861 agggcaacat cgtggagcag tattccctga acgagatcat caacaacttc aacggcatca
     1921 ggatcaagac agattaccac tctctgctgg acaagaagga gaaggagagg ttcgaggccc
     1981 gccagaactg gacctccatc gagaatatca aggagctgaa ggccggctat atctctcagg
     2041 tggtgcacaa gatctgcgag ctggtggaga agtacgatgc cgtgatcgcc ctggaggacc
     2101 tgaactctgg ctttaagaat agccgcgtga aggtggagaa gcaggtgtat cagaagttcg
     2161 agaagatgct gatcgataag ctgaactaca tggtggacaa gaagtctaat ccttgtgcaa
     2221 caggcggcgc cctgaagggc tatcagatca ccaataagtt cgagagcttt aagtccatgt
     2281 ctacccagaa cggcttcatc ttttacatcc ctgcctggct gacatccaag atcgatccat
     2341 ctaccggctt tgtgaacctg ctgaaaacca agtataccag catcgccgat tccaagaagt
     2401 tcatcagctc ctttgacagg atcatgtacg tgcccgagga ggatctgttc gagtttgccc
     2461 tggactataa gaacttctct cgcacagacg ccgattacat caagaagtgg aagctgtact
     2521 cctacggcaa ccggatcaga atcttccgga atcctaagaa gaacaacgtg ttcgactggg
     2581 aggaggtgtg cctgaccagc gcctataagg agctgttcaa caagtacggc atcaattatc
     2641 agcagggcga tatcagagcc ctgctgtgcg agcagtccga caaggccttc tactctagct
     2701 ttatggccct gatgagcctg atgctgcaga tgcggaacag catcacaggc cgcaccgacg
     2761 tggattttct gatcagccct gtgaagaact ccgacggcat cttctacgat agccggaact
     2821 atgaggccca ggagaatgcc atcctgccaa agaacgccga cgccaatggc gcctataaca
     2881 tcgccagaaa ggtgctgtgg gccatcggcc agttcaagaa ggccgaggac gagaagctgg
     2941 ataaggtgaa gatcgccatc tctaacaagg agtggctgga gtacgcccag accagcgtga
     3001 agcacggatc ctatccctat gacgtgcccg attatgccag cctgggcagc ggctccccca
     3061 agaaaaaacg caaggtggaa gatcctaaga aaaagcggaa agtggacggc attggtagtg
     3121 ggagcaacgg cagcagcctc gatgctttag acgattttga cttagatatg cttggttcag
     3181 acgcgttaga cgacttcgac ctagacatgt taggctcaga tgcattggac gacttcgatt
     3241 tagatatgtt gggctccgat gccctagatg actttgatct agatatgcta ggtagtccca
     3301 aaaagaagag gaaagtggga tccgtccttc cgcaagcgcc tgccccagcg cctgcaccgg
     3361 caatggtgtc cgccctcgca caggcccctg cgcccgtccc cgtgctcgcg cctggaccgc
     3421 cccaggcggt cgctccaccg gctccgaagc cgacgcaggc cggagaggga acactctccg
     3481 aagcacttct tcaactccag tttgatgacg aggatcttgg agcactcctt ggaaactcga
     3541 cagaccctgc ggtgtttacc gacctcgcgt cagtagataa ctccgaattt cagcagcttt
     3601 tgaaccaggg tatcccggtc gcgccacata caacggagcc catgttgatg gaataccccg
     3661 aagcaatcac gagacttgtg acgggagcgc agcggcctcc cgatcccgca cccgcacctt
     3721 tgggggcacc tggcctccct aacggacttt tgagcggcga cgaggatttc tcctccatcg
     3781 ccgatatgga tttctcagcc ttgctgtcag gctctggcag cggcagcgac ctttcccatc
     3841 cgcccccaag gggccatctg gatgagctga caaccacact tgagtccatg accgaggatc
     3901 tgaacctgga ctcacccctg accccggaat tgaacgagat tctggatacc ttcctgaacg
     3961 acgagtgcct cttgcatgcc atgcatatca gcacaggact gtccatcttc gacacatctc
     4021 tgtttggcag cagcggatcc atggtgagca agggcgagga ggataacatg gccatcatca
     4081 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     4141 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     4201 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
     4261 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     4321 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     4381 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     4441 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     4501 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
     4561 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     4621 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
     4681 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     4741 tgtacaagta agtcgacggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     4801 aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     4861 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     4921 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg accggtacca
     4981 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     5041 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     5101 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     5161 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     5221 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     5281 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     5341 ccgcgtggtt cctggccacc gtcggcgttt ctccagacca ccagggcaag ggtctgggca
     5401 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     5461 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     5521 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctaaa
     5581 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5641 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5701 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5761 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5821 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5881 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5941 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     6001 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6061 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6121 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag
     6181 ctcggcctcg agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
     6241 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     6301 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     6361 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6421 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6481 agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc
     6541 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
     6601 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
     6661 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
     6721 ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta
     6781 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     6841 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     6901 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     6961 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     7021 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
     7081 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     7141 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     7201 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     7261 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     7321 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7381 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7441 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7501 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7561 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7621 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7681 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7741 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7801 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7861 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7921 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7981 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8041 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8101 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8161 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8221 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8281 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8341 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8401 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8461 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8521 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8581 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8641 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8701 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8761 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8821 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8881 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8941 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9001 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9061 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9121 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9181 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9241 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     9301 tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     9361 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     9421 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     9481 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9541 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     9601 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga
     9661 caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg
     9721 taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc
     9781 gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt
     9841 cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc
     9901 gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt
     9961 ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
    10021 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
    10081 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga
    10141 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
    10201 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg
    10261 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac
    10321 tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg
    10381 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
    10441 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
    10501 tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
    10561 atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
    10621 gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac
    10681 tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata
    10741 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
    10801 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
    10861 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
    10921 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
    10981 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
    11041 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
    11101 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
    11161 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
    11221 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
    11281 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
    11341 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
    11401 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    11461 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    11521 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    11581 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
    11641 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    11701 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
    11761 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
    11821 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
    11881 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
    11941 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
    12001 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
    12061 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
    12121 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
    12181 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    12241 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    12301 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
    12361 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
    12421 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
    12481 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
    12541 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
    12601 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    12661 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    12721 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    12781 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    12841 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
    12901 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
    12961 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
    13021 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
    13081 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
    13141 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
    13201 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
    13261 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
    13321 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
    13381 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
    13441 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
    13501 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
    13561 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
    13621 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
    13681 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
    13741 atcgccaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
    13801 gtgcagggga aagaatagta gacataa
//
