LOCUS       sequence-292015-        6679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for the  MS2-GFP protein in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Wosika V, Pelet S
  TITLE     Single-particle imaging of stress-promoters induction reveals the 
            interplay between MAPK signaling, chromatin and transcription 
            factors.
  JOURNAL   Nat Commun. 2020 Jun 23;11(1):3171. doi: 10.1038/s41467-020-16943-w.
  PUBMED    32576833
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-06-23"; volume: "11(1):3171. doi"; pages: " 
            10.1038/s41467-020-16943-w"
COMMENT     Sequence Label: pSP561
FEATURES             Location/Qualifiers
     source          1..6679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     251..268
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     252..268
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(297..313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(297..313)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    314..347
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        384..727
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      1546..1743
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    1767..1800
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1801..1820
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1801..1819
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2584..3288
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     terminator      4397..4644
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4413..4431)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(4667..4685)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(4973..5561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5053..5072)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5732..6523,6524..6592))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDCW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6355..6374
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6593..6656)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgatgatatt tcataaataa tgtaattcta tatatgttaa ttaccttttt tgcgaggcat
       61 atttatggtg aaggataagt tttgaccatc aaagaaggtt aatgtggctg tggtttcagg
      121 gtccataatg cttttcaatt catctttttt ttttttgttc ttttttttga ttccggtttc
      181 tttgaaattt ttttgattcg gtaatctccg agcagaagga agaacgaagg aaggagcaca
      241 gacttagatt tgtaaaacga cggccagtgc atgcgtatcg gacgtctccc ttcatggtca
      301 tagctgtttc ctgataactt cgtataatgt atgctatacg aagttattag cttgcctcgt
      361 ccccgccggg tcacccggcg agcgacatgg aggcccagaa taccctcctt gacagtcttg
      421 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc
      481 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg
      541 gctcctcgct ccagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc
      601 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct
      661 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata
      721 aacaaccatg acagtcaaca ctaataccta tagtgagaga gcagaaactc atgcctcacc
      781 agtagcacag cgattatttc gattaatgga actgaagaaa accaatttat gtgcatcaat
      841 tgacgttgat accactaagg agttccttga attaattgat aaattaggtc cttatgtatg
      901 cttaatcaag actcatattg atataatcaa tgatttttcc tatgaatcca ctattgaacc
      961 attattagaa ctttcacgta aacatcaatt tatgattttt gaagatagaa aatttgctga
     1021 tattggtaat accgtaaaga aacaatatat tggtggagtt tataaaatta gtagttgggc
     1081 agatattacc aatgctcatg gtgtcactgg gaatggagtg gttgaaggat taaaacaggg
     1141 agctaaagaa accaccacca accaagagcc aagagggtta ttgatgttag ctgaattatc
     1201 atcagtggga tcattagcat acggagaata ttctcaaaaa actgttgaaa ttgctaaatc
     1261 cgataaggaa tttgttattg gatttattgc ccaacgtgat atgggtggcc aagaagaagg
     1321 atttgattgg cttattatga cacctggagt tggattagat gataaaggtg atggattagg
     1381 acaacaatat agaactgttg atgaagttgt tagcactgga actgatatta tcattgttgg
     1441 tagaggattg tttggtaaag gaagagatcc agatattgaa ggtaaaaggt atagaaatgc
     1501 tggttggaat gcttatttga aaaagactgg ccaattataa tcagtactga caataaaaag
     1561 attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt
     1621 taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg
     1681 aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata
     1741 ctgctgtcga ttcgatacta acgccgataa cttcgtataa tgtatgctat acgaagttat
     1801 taatacgact cactataggg cgaattggag ctcgccggga tcgaagaaat gatggtaaat
     1861 gaaataggaa atcaaggagc atgaaggcaa aagacaaata taagggtcga acgaaaaata
     1921 aagtgaaaag tgttgatatg atgtatttgg ctttgcggcg ccgaaaaaac gagtttacgc
     1981 aattgcacaa tcatgctgac tctgtggcgg acccgcgctc ttgccggccc ggcgataacg
     2041 ctgggcgtga ggctgtgccc ggcggagttt tttgcgcctg cattttccaa ggtttaccct
     2101 gcgctaaggg gcgagattgg agaagcaata agaatgccgg ttggggttgc gatgatgacg
     2161 accacgacaa ctggtgtcat tatttaagtt gccgaaagaa cctgagtgca tttgcaacat
     2221 gagtatacta gaagaatgag ccaagacttg cgagacgcga gtttgccggt ggtgcgaaca
     2281 atagagcgac catgaccttg aaggtgagac gcgcataacc gctagagtac tttgaagagg
     2341 aaacagcaat agggttgcta ccagtataaa tagacaggta catacaacac tggaaatggt
     2401 tgtctgtttg agtacgcttt caattcattt gggtgtgcac tttattatgt tacaatatgg
     2461 aagggaactt tacacttctc ctatgcacat atattaatta aagtccaatg ctagtagaga
     2521 aggggggtaa cacccctccg cgctcttttc cgattttttt ctaaaccgtg gaatatttcg
     2581 gatatccttt tgttgtttcc gggtgtacaa tatggacttc ctcttttctg gcaaccaaac
     2641 ccatacatcg ggattcctat aataccttcg ttggtctccc taacatgtag gtggcggagg
     2701 ggagatatac aatagaacag ataccagaca agacataatg ggctaaacaa gactacacca
     2761 attacactgc ctcattgatg gtggtacata acgaactaat actgtagccc tagacttgat
     2821 agccatcatc atatcgaagt ttcactaccc tttttccatt tgccatctat tgaagtaata
     2881 ataggcgcat gcaacttctt ttcttttttt ttcttttctc tctcccccgt tgttgtctca
     2941 ccatatccgc aatgacaaaa aaatgatgga agacactaaa ggaaaaaatt aacgacaaag
     3001 acagcaccaa cagatgtcgt tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa
     3061 ctttttcctt ccttcattca cgcacactac tctctaatga gcaacggtat acggccttcc
     3121 ttccagttac ttgaatttga aataaaaaaa agtttgctgt cttgctatca agtataaata
     3181 gacctgcaat tattaatctt ttgtttcctc gtcattgttc tcgttccctt tcttccttgt
     3241 ttctttttct gcacaatatt tcaagctata ccaagcatac aatcaactcc aagctggccg
     3301 ctctagaatg gcttctaact ttactcagtt cgttctcgtc gacaatggcg gaactggcga
     3361 cgtgactgtc gccccaagca acttcgctaa cgggatcgct gaatggatca gctctaactc
     3421 gcgttcacag gcttacaaag taacctgtag cgttcgtcag agctctgcgc agaatcgcaa
     3481 atacaccatc aaagtcgagg tgcctaaagg cgcctggcgt tcgtacttaa atatggaact
     3541 aaccattcca attttcgcca cgaattccga ctgcgagctt attgttaagg caatgcaagg
     3601 tctcctaaaa gatggaaacc cgattccctc agcaatcgca gcaaactccg gcatctacaa
     3661 gcttatgtct aaaggcgagg aattgtttac aggtgtagtt cctattttgg tcgaactaga
     3721 tggagatgtc aacggtcata agttttctgt acgtggtgaa ggcgaaggtg atgctactaa
     3781 tggcaaactg actctgaagt tcatctgtac gaccggaaaa ttgccagttc catggcctac
     3841 attagtaaca acattatgtt acggagttca atgctttgct agatacccag atcacatgaa
     3901 gcagcatgac tttttcaaaa gtgctatgcc agaaggttat gtgcaggaga gaactatctc
     3961 cttcaaagat gatggtactt ataagacaag agcagaggta aagtttgaag gcgatacatt
     4021 ggttaataga attgagctta aaggcattga ttttaaggaa gatgggaata tcctcggaca
     4081 taaacttgaa tacaatttca attcacataa tgtttatatc acagcagata aacaaaagaa
     4141 tggtataaaa gcgaacttta agacaagaca taacgtggaa gatggtagcg tccaattagc
     4201 cgaccattac caacaaaaca ctcctatcgg ggacgggcca gttctccttc cagacaacca
     4261 ctacctatca acccaatctg ttctatctaa agaccctaat gaaaaacgag atcacatggt
     4321 cttgttagag ttcgtgactg ccgctggtat aacattagga atggacgaat tgtacaaata
     4381 aggatcctaa ctcgagtcat gtaattagtt atgtcacgct tacattcacg ccctcccccc
     4441 acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat
     4501 ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt
     4561 ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg
     4621 ggacgctcga aggctttaat ttgcggccgg tacccagctt ttgttccctt tagtgagggt
     4681 taatttcctg aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc
     4741 ttcaatttaa ttatatcagt tattacgcgg gaatctcggt cgtaatgatt tctataatga
     4801 cgaaaaaaaa aaaattggaa agaaaatgct tcatggcctt tataaaaagg aactatccaa
     4861 tacctcgcca gaaccaagta acagtatttt acggggcaca aatcaagaac aataagacag
     4921 gactgtaaag atggacgcat ttaattaatt cgaaggccgc gttgctggcg tttttccata
     4981 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5041 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5101 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5161 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5221 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5401 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5521 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5581 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5641 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5701 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5761 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5821 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5881 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5941 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6001 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6061 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacaa tcaaggcgag
     6121 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6181 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6241 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6301 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6361 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6421 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6481 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6541 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6601 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6661 aagtatcgtt cgaattaat
//
