LOCUS       sequence-292039-        5283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and 
            rbcS-E9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Jores T, Tonnies J, Dorrity MW, Cuperus J, Fields S, Queitsch C
  TITLE     Identification of Plant Enhancers and Their Constituent Elements by 
            STARR-seq in Tobacco Leaves.
  JOURNAL   Plant Cell. 2020 May 14. pii: tpc.20.00155. doi: 
            10.1105/tpc.20.00155.
  PUBMED    32409318
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2020 May 14. pii: tpc.20.00155. doi: 10.1105/tpc.20.00155."
COMMENT     Sequence Label: pZS*11_4enh
FEATURES             Location/Qualifiers
     source          1..5283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      446..668
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(1400..1494)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1683..2474,2475..2543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2306..2325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2544..2648)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(2992..3010,3011..3016,3017..3033,3034..3039,
                     3040..3065)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    2992..3010
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3017..3035
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             3078..3100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      4106..4192
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(4731..5283,1..398))
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa tatctcttca
       61 ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca ctggaaaatc
      121 tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag ctctctggtt
      181 gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg agcgtattgg
      241 ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt ggggttgagt
      301 agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata gcgactaatc
      361 gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg gtctaggtga
      421 ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt ccttttcccg
      481 ggagatctgg gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct
      541 agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtttatat tcaagtggtt
      601 ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg
      661 tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt
      721 gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg
      781 ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt
      841 tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta
      901 caggcgccac actaacatcg aatgtactta tctcattagt ttaaattatt gtttgatcat
      961 gtttaatcct tatactgttg ttagtttttt cagttagctt agtgggcatc ttacacgtgg
     1021 cattattatc ctattggtgg ctaatgataa ggttagcaca caaaactttt caatcttgtg
     1081 tggttaatat gactgcaaag tttatcattt tcacaatcca acaaactggt tctaggcagt
     1141 ggggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa agcccgtcac
     1201 gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac tttttgctgt
     1261 tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc cgtgacaggt
     1321 cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc ttacccgtct
     1381 tactgtccct agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     1441 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     1501 agctcagatc tctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     1561 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgacatctcc
     1621 actgacgtaa gggatgacgc acaatcccac tatccttcga gctcgtaaac ttggtctgac
     1681 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1741 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1801 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1861 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1921 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1981 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2041 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2101 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2161 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2221 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2281 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2341 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2401 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2461 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2521 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2581 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2641 gttccgcgca catttccccg aaaagtgcca cctgacgtcc aacatatggc accggaggct
     2701 ttcgtcttca cctcgagatc gaatttgttg ggcaagcgcc cagtgtgatg tacgttggga
     2761 aaacttgcaa gaggatgcga ccaaatgaac tggtaaacca tcccgtgagc gtggcctaca
     2821 cattttaagc cagcggactc tttcgacttg tcttacaaaa gctggtccag tcacgagcct
     2881 tagccctaac catagccaca agtacatctc atccatttaa ggcctctgcg tgcaccaatg
     2941 gcatccaagc tgcagatttc ttttcaccac cgtctctctt gtcagctcga gtccctatca
     3001 gtgatagaga ttgacatccc tatcagtgat agagatactg agcacatcag caggacgcac
     3061 tgaccgaatt caataatttt gtttaacttt aagaaggaga tggtaccatg agcaaaggtg
     3121 aagaactgtt caccggcgtt gtgccaattc tggttgagct ggatggtgac gtgaatggcc
     3181 acaaattttc cgtgtctggt gaaggcgagg gtgatgctac ttatggcaaa ctgactctga
     3241 aactgatctg taccaccggc aaactgcctg ttccatggcc aactctggtc actactctgg
     3301 gttacggcct gatgtgtttt gcgcgttacc cggatcacat gaaacagcat gacttcttca
     3361 aatctgccat gccggaaggc tatgtccaag aacgtacgat ctttttcaag gacgacggca
     3421 actataaaac ccgtgccgaa gttaaattcg agggtgacac cctggttaac cgcatcgaac
     3481 tgaaaggcat tgacttcaaa gaggacggca acattctggg tcacaagctg gaatacaact
     3541 acaactccca caacgtttac attactgctg acaagcagaa aaacggcatc aaagcaaact
     3601 tcaagatccg tcacaacatt gaagatggtg gcgtacagct ggcagatcac taccagcaga
     3661 acactccaat cggtgatggc ccagtactgc tgccagataa ccattacctg tcctaccaga
     3721 gcaaactgtc taaagacccg aacgaaaaac gtgaccacat ggtactgctg gaatttgtta
     3781 ccgcggcagg cattacccac ggtatggacg aactgtataa atagaagctt aattagctga
     3841 gtctagactg ccataatgtc acaatttttc tcaaatcttg tggctctcaa acactgtata
     3901 aaacacgaca aatgtggacc caaaatatat accttacact tctgagttag agaagcagag
     3961 ccccataatt aagcctattt tatgaaaaaa ataatattat gttgagtcat atatccataa
     4021 gaatccccac agtcacacat ggaagagcag cattggatac aaatgatatg aagattttga
     4081 tccatgaacg gatttctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     4141 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc
     4201 ctagacctag ggtacgggtt ttgctgcccg caaacgggct gttctggtgt tgctagtttg
     4261 ttatcagaat cgcagatccg gcttcagccg gttttgccgg ctgaaagcgc tatttcttcc
     4321 agaattgcca tgattttttc cccacgggag gcgtcactgg ctcccgtgtt gtcggcagct
     4381 ttgattcgat aagcagcatc gcctgtttca ggctgtctat gtgtgactgt tgagctgtaa
     4441 caagttgtct caggtgttca atttcatgtt ctagttgctt tgttttactg gtttcacctg
     4501 ttctattagg tgttacatgc tgttcatctg ttacattgtc gatctgttca tggtgaacag
     4561 ctttgaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt acaccgtttt
     4621 catctgtgca tatggacagt tttccctttg atatgtaacg gtgaacagtt gttctacttt
     4681 tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt
     4741 ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac
     4801 gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc aaaactggtg
     4861 agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag
     4921 gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca
     4981 agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc
     5041 gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt
     5101 attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt
     5161 aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt
     5221 gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta
     5281 aca
//
