LOCUS       sequence-292068-        9233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human HOXA9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 9233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-HOXA9
FEATURES             Location/Qualifiers
     source          1..9233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    167..284
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(341..359,360..382,383..401,402..424,425..443,444..466,
                     467..485,486..508,509..527,528..550,551..569,570..592,
                     593..611)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        644..682
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     679..703
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1034..1051
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1601..1624
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1652..2240
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1705..1725)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2123..2134)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2243..2259)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2244..2260)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2765..2945
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2971..2988)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2977..3201
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3247..3675
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3334..3353)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3544..3565
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3684..3704)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3689..4018
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3869..4004
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3931..3950
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4066..4113
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4132..4506
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPGMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4636..4757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4673..4692)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4727..4746
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4806..4822)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4806..4822)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4819..4841)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4830..4846
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4854..4860,4861..4878,4879..4884))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4899..4920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5037..5054)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5208..5796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5288..5307)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5967..6758,6759..6827))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6590..6609
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6828..6932)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7007..7026)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7198..7577
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7579..7777
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7795..7975
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8022..8147
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8640..8873
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9058..9102
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
       61 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
      121 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      181 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      241 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      301 acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa
      361 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      421 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
      481 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      541 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      601 agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg
      661 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
      721 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt
      781 caccatggcc accactgggg ccctgggcaa ctactacgtg gactcgttcc tgctgggcgc
      841 cgacgccgcg gatgagctga gcgttggccg ctatgcgccg gggaccctgg gccagcctcc
      901 ccggcaggcg gcgacgctgg ccgagcaccc cgacttcagc ccgtgcagct tccagtccaa
      961 ggcgacggtg tttggcgcct cgtggaaccc agtgcacgcg gcgggcgcca acgctgtacc
     1021 cgctgcggtg taccaccacc atcaccacca cccctacgtg cacccccagg cgcccgtggc
     1081 ggcggcggcg ccggacggca ggtacatgcg ctcctggctg gagcccacgc ccggtgcgct
     1141 ctccttcgcg ggcttgccct ccagccggcc ttatggcatt aaacctgaac cgctgtcggc
     1201 cagaaggggt gactgtccca cgcttgacac tcacactttg tccctgactg actatgcttg
     1261 tggttctcct ccagttgata gagaaaaaca acccagcgaa ggcgccttct ctgaaaacaa
     1321 tgctgagaat gagagcggcg gagacaagcc ccccatcgat cccaataacc cagcagccaa
     1381 ctggcttcat gcgcgctcca ctcggaaaaa gcggtgcccc tatacaaaac accagaccct
     1441 ggaactggag aaagagtttc tgttcaacat gtacctcacc agggaccgca ggtacgaggt
     1501 ggctcgactg ctcaacctca ccgagaggca ggtcaagatc tggttccaga accgcaggat
     1561 gaaaatgaag aaaatcaaca aagaccgagc aaaagacgag gattacaagg atgacgacga
     1621 taagtgagaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     1681 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     1741 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     1801 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     1861 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     1921 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     1981 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     2041 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     2101 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     2161 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     2221 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
     2281 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     2341 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     2401 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     2461 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     2521 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     2581 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     2641 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     2701 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     2761 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     2821 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     2881 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     2941 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     3001 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     3061 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3121 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     3181 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     3241 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3301 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3361 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3421 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3481 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3541 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     3601 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     3661 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     3721 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     3781 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3841 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3901 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     3961 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     4021 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     4081 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     4141 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     4201 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     4261 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     4321 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     4381 acgaacttcc gggacgcctc cgggccgggc atgaccgaga tcggcgagca gccgtggggg
     4441 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     4501 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     4561 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     4621 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4681 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4741 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     4801 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     4861 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     4921 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     4981 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5041 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5101 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5161 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5221 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5281 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5341 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5401 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5461 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5521 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5581 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     5641 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     5701 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     5761 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     5821 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     5881 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     5941 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6001 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6061 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6121 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6181 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6241 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6301 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6361 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6421 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6481 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     6541 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     6601 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     6661 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     6721 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     6781 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     6841 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     6901 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     6961 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     7021 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     7081 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     7141 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     7201 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     7261 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     7321 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     7381 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     7441 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     7501 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     7561 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     7621 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     7681 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     7741 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
     7801 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7861 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7921 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     7981 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     8041 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     8101 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     8161 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     8221 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     8281 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     8341 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     8401 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     8461 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     8521 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     8581 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     8641 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     8701 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     8761 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     8821 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     8881 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     8941 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     9001 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     9061 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     9121 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     9181 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttc
//
