LOCUS       sequence-292072-       10262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of mouse Nfat1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 10262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-Nfat1
FEATURES             Location/Qualifiers
     source          1..10262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    167..284
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(341..359,360..382,383..401,402..424,425..443,444..466,
                     467..485,486..508,509..527,528..550,551..569,570..592,
                     593..611)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        644..682
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     679..703
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2681..3269
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2734..2754)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3152..3163)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3272..3288)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3273..3289)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3794..3974
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4000..4017)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4006..4230
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4276..4704
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4363..4382)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4573..4594
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4713..4733)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4718..5047
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4898..5033
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4960..4979
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5095..5142
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5161..5535
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5665..5786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5702..5721)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5756..5775
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5835..5851)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5835..5851)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5848..5870)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5859..5875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5883..5889,5890..5907,5908..5913))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5928..5949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6066..6083)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6237..6825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6317..6336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6996..7787,7788..7856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7619..7638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7857..7961)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8036..8055)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8227..8606
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8608..8806
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8824..9004
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9051..9176
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9669..9902
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10087..10131
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
       61 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
      121 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      181 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      241 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      301 acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa
      361 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      421 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
      481 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      541 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      601 agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg
      661 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
      721 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt
      781 gatggacgtc ccggagccgc agcccgaccc cgatggcggg gacggccccg gccacgagcc
      841 cgggggcagt ccccaagacg agctggactt ttccatcctc ttcgattatg actatctgaa
      901 ccctatcgaa gaagaaccga tcgcacataa ggccatcagc tcaccctccg gactcgcata
      961 cccggatgat gtcctggact atggcctcaa gccatgcaac ccccttgcca gtctctctgg
     1021 cgagccccct ggccggttcg gagagccgga tagtataggg ttccagaact ttctgagccc
     1081 ggtcaagcca gcaggggctt cgggcccgag ccctcggatc gagatcactc catcccacga
     1141 actgatgcag gcaggggggg ccctccgtgg gagagacgcc ggcctgtccc ccgagcagcc
     1201 ggccctggcc ctggccggcg tggccgccag cccgaggttc acactgcccg tgcccggcta
     1261 cgagggctac cgcgagccgc tttgcttgag ccccgctagc agcggctcct ctgccagctt
     1321 catttctgac accttctccc cctacacctc gccctgcgtc tcacccaata acgccgggcc
     1381 cgacgacctg tgtccccagt ttcaaaacat ccctgctcat tattccccca gaacctctcc
     1441 aataatgtca cctcgaacca gcctcgccga ggacagctgc ctgggccgac actcgcccgt
     1501 gccccgtccg gcatcccgct cctcctcacc cggtgccaag cggaggcatt cgtgcgcaga
     1561 ggctttggtt gctcctctgc ccgcagcctc accccagcgc tcccggagcc cctcgccaca
     1621 gccctcgcct cacgtggcac tgcaggacga cagcatcccc gctgggtacc cccccacggc
     1681 cggctctgct gttctcatgg atgccctcaa caccctggcc accgactcgc cctgcgggat
     1741 cccctccaag atatggaaga ccagtcctga cccgacgcct gtgtccaccg ctccgtccaa
     1801 ggctggcctg gcccgccaca tctaccctac tgtggagttc ctggggccat gtgagcagga
     1861 ggagaggagg aattccgctc cagagtccat cctgctggta ccacctactt ggcccaagca
     1921 gttggtgccg gccattccca tctgcagcat ccctgtgact gcatccctcc caccactcga
     1981 gtggccactc tccaatcagt cgggctccta tgagctacgg attgaggtcc aacccaagcc
     2041 ccatcaccgg gcccactatg agacggaggg cagccgtggc gctgtcaaag ccccaacagg
     2101 aggacaccct gtggtgcagc tccacggcta catggagaac aagcctctgg ggcttcagat
     2161 cttcattggg acagcagatg agaggatcct taagccgcac gccttctacc aagtacacag
     2221 gatcactggg aaaacggtca ccaccacgag ctatgagaag atcgtaggca acaccaaggt
     2281 cctggagatc cccctggagc caaagaacaa catgagagcc accatcgact gtgcaggcat
     2341 cctgaagctc cgaaacgctg acatcgagct gcggaagggc gagacggaca tcggcaggaa
     2401 gaacacgcgt gtgcgcctgg tgttccgcgt gcacgtccca gagcccagtg ggcgcatcgt
     2461 ctccctgcag gctgcgtcca accccatcga gtgctgtaag tcagggctga ggaaggggca
     2521 tgtgagcatc ccccctgggc aggtgacctg caggaatcca ccggtctgtg gtgacccaaa
     2581 gggcagacac tgtgcccagg ttttgatgtc tgcttctgtc tgcttgtgga gacttctctc
     2641 aggaaataaa acatagcaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     2701 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2761 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2821 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2881 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     2941 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3001 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3061 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     3121 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3181 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3241 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
     3301 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     3361 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     3421 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     3481 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     3541 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     3601 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     3661 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     3721 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     3781 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     3841 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     3901 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     3961 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     4021 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4081 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4141 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4201 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     4261 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4321 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4381 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4441 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4501 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4561 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     4621 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4681 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     4741 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4801 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4861 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4921 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4981 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5041 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     5101 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     5161 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     5221 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     5281 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     5341 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     5401 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     5461 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     5521 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     5581 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     5641 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     5701 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5761 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     5821 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5881 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     5941 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6001 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6061 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6661 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7981 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     8041 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     8101 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     8161 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     8221 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     8281 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     8341 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     8401 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     8461 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     8521 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     8581 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     8641 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     8701 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     8761 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
     8821 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8881 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8941 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     9001 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     9061 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     9121 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     9181 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     9241 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     9301 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     9361 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     9421 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     9481 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     9541 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     9601 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     9661 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     9721 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     9781 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     9841 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     9901 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     9961 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    10021 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    10081 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    10141 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    10201 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    10261 tc
//
