LOCUS       sequence-292073-       10769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of mouse Stat5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10769)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 10769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-Stat5
FEATURES             Location/Qualifiers
     source          1..10769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    142..259
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(316..334,335..357,358..376,377..399,400..418,419..441,
                     442..460,461..483,484..502,503..525,526..544,545..567,
                     568..586)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        619..657
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     654..678
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3163..3751
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3216..3236)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3634..3645)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3754..3770)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3755..3771)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4276..4456
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4482..4499)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4488..4712
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4758..5186
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4845..4864)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5055..5076
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5195..5215)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5200..5529
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5380..5515
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5442..5461
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5577..5624
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5643..6017
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6147..6268
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6184..6203)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6238..6257
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6317..6333)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6317..6333)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6330..6352)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6341..6357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6365..6371,6372..6389,6390..6395))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6410..6431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6548..6565)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6719..7307)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6799..6818)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7478..8269,8270..8338))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8101..8120
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8339..8443)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8518..8537)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8709..9088
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9090..9288
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9306..9486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9533..9658
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10151..10384
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10569..10613
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
       61 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
      121 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
      181 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
      241 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaga
      301 tctcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
      361 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
      421 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
      481 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      541 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      601 gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt
      661 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      721 caccgggacc gatccagcct ccgcggcccc gaattcatgg cgggctggat tcaggcccag
      781 cagcttcagg gagatgccct gcgccagatg caagtgttgt atgggcagca tttccccatc
      841 gaggtccggc actacctggc ccagtggatc gagagccagc cgtgggatgc tattgacttg
      901 gataatcccc aggaccgagg tcaggccacc caactcctgg agggcctggt gcaggagctg
      961 cagaagaagg cggagcacca ggtgggggaa gatgggtttt tgctgaagat caagctgggg
     1021 cactatgcca cacagctcca gaacacgtat gaccgctgtc ccatggagct ggttcgctgt
     1081 atccgtcaca ttctgtacaa cgaacagagg ctggttcgcg aagccaacaa ttgcagctcc
     1141 cctgctggtg tcctggttga cgccatgtcc cagaagcacc ttcagatcaa ccaaaggttt
     1201 gaggagctgc gcctgatcac acaggacacg gagaacgagc tgaagaagct gcagcagacc
     1261 caagagtact tcatcatcca gtaccaggag agcctgcgga tccaagctca gtttgcccag
     1321 ctgggccagc tgaaccccca ggagcgcatg agcagggaga cggccctcca gcagaagcaa
     1381 gtgtccctgg agacctggct gcagcgagag gcacagacac tgcagcagta ccgagtggag
     1441 ctggctgaga agcaccagaa gaccctgcag ctgctgcgga agcagcagac catcatcctg
     1501 gacgacgagc tgatccagtg gaagcggaga cagcagctgg ccgggaacgg gggtcccccc
     1561 gagggcagcc tggacgtgct gcagtcctgg tgtgagaagc tggccgagat catctggcag
     1621 aaccggcagc agatccgcag ggctgagcac ctgtgccagc agctgcccat cccaggcccc
     1681 gtggaggaga tgctggctga ggtcaacgcc accatcacgg acatcatctc agctctggtc
     1741 accagcacgt tcatcatcga gaagcagcct cctcaggtcc tgaagaccca gaccaagttt
     1801 gcggccaccg tgcgcctgct ggtgggggga aagctgaatg tgcacatgaa ccccccgcag
     1861 gtgaaggcga ccatcatcag cgagcagcag gccaagtccc tgctcaagaa tgagaacacc
     1921 cgcaatgagt gcagcggcga gatcctgaac aactgttgcg tcatggagta ccaccaggcc
     1981 actggcacgc tcagcgccca cttcagaaac atgtcactga aaagaatcaa gcgcgccgac
     2041 aggcgtggtg cagagtcggt gacggaggag aagttcacag tcctgtttga gtctcagttc
     2101 agcgttggca gcaacgagct ggtgttccag gtgaagaccc tgtccctccc tgtggtcgtt
     2161 atcgtccatg gcagccagga ccacaatgct actgccaccg tgctgtggga caatgccttt
     2221 gctgagccgg gcagggtgcc atttgctgtg cctgacaagg tgctgtggcc gcagctgtgt
     2281 gaagcgctca acatgaaatt caaggctgaa gtacagagca accggggctt gaccaaagag
     2341 aacctcgtgt tcctggcaca gaaactgttc aacatcagca gcaaccacct cgaggactac
     2401 aacagcatgt ctgtgtcctg gtcccagttc aaccgggaga acttgcccgg ctggaactac
     2461 accttctggc agtggttcga cggggtgatg gaggtgctga agaagcacca taagccccat
     2521 tggaatgatg gggctatcct gggtttcgtg aacaagcaac aggcccacga cctgctcatc
     2581 aacaagccgg acgggacctt cctgctgcgc ttcagtgact cggaaatcgg gggcatcacc
     2641 attgcttgga agtttgactc tccggaccga aacctctgga atctgaagcc attcacgacg
     2701 cgagatttct ccattcggtc cctggccgac cggctggggg acctgaacta ccttatctac
     2761 gtgttcccag accgacccaa ggacgaggtc tttgccaagt attacactcc tgtacttgcg
     2821 aaagcagttg acggatacgt gaagccacag atcaagcaag tggtccctga gttcgtcaat
     2881 gcatccacag atgccggagc cagcgccacc tacatggacc aggctccttc cccagtcgtg
     2941 tgccctcaac ctcactacaa catgtaccca cccaaccctg accctgtcct tgaccaagat
     3001 ggcgagtttg acctggatga gagcatggat gttgccaggc acgtggaaga acttttacgc
     3061 cggcccatgg acagtctcga cgcccgcctc tccccacctg ctggtctctt cacctccgct
     3121 agaagctccc tgtcctgaga attcgatatc aagcttatcg ataatcaacc tctggattac
     3181 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3241 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3301 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3361 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     3421 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3481 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3541 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     3601 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     3661 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3721 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa
     3781 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     3841 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     3901 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     3961 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     4021 ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc
     4081 tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa
     4141 ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     4201 gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg
     4261 catccggact gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     4321 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     4381 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     4441 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     4501 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     4561 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     4621 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     4681 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     4741 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4801 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4861 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4921 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     4981 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5041 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5101 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5161 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     5221 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5281 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5341 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5401 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5461 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5521 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     5581 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     5641 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     5701 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     5761 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     5821 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     5881 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     5941 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     6001 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     6061 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     6121 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     6181 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6241 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     6301 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6361 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6421 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6481 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6541 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6601 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6661 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6721 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6781 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6841 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6901 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6961 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7021 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7081 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7141 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7201 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7261 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7321 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7381 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7441 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7501 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     7561 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7621 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7681 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7741 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7801 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7861 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7921 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7981 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8041 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     8101 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8161 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     8221 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8281 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     8341 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8401 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8461 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
     8521 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     8581 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     8641 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
     8701 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     8761 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     8821 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     8881 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     8941 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     9001 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     9061 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     9121 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     9181 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     9241 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct
     9301 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     9361 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     9421 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     9481 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     9541 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     9601 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     9661 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     9721 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     9781 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     9841 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     9901 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     9961 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
    10021 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
    10081 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
    10141 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
    10201 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
    10261 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
    10321 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
    10381 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
    10441 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
    10501 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
    10561 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
    10621 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
    10681 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
    10741 attcaccatt atcgtttcag acccacctc
//
