LOCUS       sequence-292075-       10553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of mouse Cebpa in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 10553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-Cebpa
FEATURES             Location/Qualifiers
     source          1..10553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    167..284
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(341..359,360..382,383..401,402..424,425..443,444..466,
                     467..485,486..508,509..527,528..550,551..569,570..592,
                     593..611)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        644..682
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     679..703
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2972..3560
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3025..3045)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3443..3454)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3563..3579)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3564..3580)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4085..4265
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4291..4308)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4297..4521
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4567..4995
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4654..4673)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4864..4885
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5004..5024)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5009..5338
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5189..5324
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5251..5270
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5386..5433
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5452..5826
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5956..6077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5993..6012)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6047..6066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6126..6142)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6126..6142)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6139..6161)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6150..6166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6174..6180,6181..6198,6199..6204))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6219..6240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6357..6374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6528..7116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6608..6627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7287..8078,8079..8147))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7910..7929
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8148..8252)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8327..8346)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8518..8897
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8899..9097
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9115..9295
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9342..9467
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9960..10193
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10378..10422
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
       61 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
      121 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      181 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      241 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      301 acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa
      361 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      421 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
      481 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      541 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      601 agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg
      661 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
      721 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt
      781 catggagtcg gccgacttct acgaggtgga gccgcggccc ccgatgagca gtcacctcca
      841 gagccccccg cacgcgccca gcaacgccgc ctttggcttt ccccggggcg cgggccccgc
      901 gccgccccca gccccacctg ccgccccgga gccgctgggc ggcatctgcg agcacgagac
      961 gtctatagac atcagcgcct acatcgaccc ggccgccttc aacgacgagt tcctggccga
     1021 cctcttccag cacagccgac agcaggagaa ggccaaggcg gcggcgggcc ccgcgggtgg
     1081 cggcggtgac tttgactacc cgggagcccc ggcgggcccc ggcggcgcgg tcatgtccgc
     1141 gggggcgcac gggccccctc ccggctacgg ctgtgcggcg gccggctacc tggacggcag
     1201 gctggagccc ctgtacgagc gcgtcggggc gcccgcgcta cggccgctgg tgatcaaaca
     1261 agagccccgc gaggaggacg aggcgaagca gctggcgctg gccggcctct tcccctacca
     1321 gccaccgccg ccaccgccac cgccgcaccc gcacgcgtct cccgcgcacc tggccgcccc
     1381 ccacttgcag ttccagatcg cgcactgcgg ccagaccacc atgcacctgc agcctggcca
     1441 ccccacaccg ccgcccacgc ccgtgcccag cccgcacgct gcgcccgcct tgggtgctgc
     1501 gggcctgcct ggccccggga gcgcgctcaa gggcttggcc ggtgcgcacc ccgacctccg
     1561 cacgggaggc ggcggcggtg gcagcggtgc cggtgcgggc aaagccaaga agtcggtgga
     1621 caagaacagc aacgagtacc gggtacggcg ggaacgcaac aacatcgcgg tgcgcaagag
     1681 ccgagataaa gccaaacaac gcaacgtgga gacgcaacag aaggtgctgg agttgaccag
     1741 tgacaatgac cgcctgcgca agcgggtgga acagctgagc cgtgaactgg acacgctgcg
     1801 gggcatcttc cgccagctgc ctgagagctc cttggtcaag gccatgggca actgcgcgtg
     1861 agaattcatg gagtcggccg acttctacga ggtggagccg cggcccccga tgagcagtca
     1921 cctccagagc cccccgcacg cgcccagcaa cgccgccttt ggctttcccc ggggcgcggg
     1981 ccccgcgccg cccccagccc cacctgccgc cccggagccg ctgggcggca tctgcgagca
     2041 cgagacgtct atagacatca gcgcctacat cgacccggcc gccttcaacg acgagttcct
     2101 ggccgacctc ttccagcaca gccgacagca ggagaaggcc aaggcggcgg cgggccccgc
     2161 gggtggcggc ggtgactttg actacccggg agccccggcg ggccccggcg gcgcggtcat
     2221 gtccgcgggg gcgcacgggc cccctcccgg ctacggctgt gcggcggccg gctacctgga
     2281 cggcaggctg gagcccctgt acgagcgcgt cggggcgccc gcgctacggc cgctggtgat
     2341 caaacaagag ccccgcgagg aggacgaggc gaagcagctg gcgctggccg gcctcttccc
     2401 ctaccagcca ccgccgccac cgccaccgcc gcacccgcac gcgtctcccg cgcacctggc
     2461 cgccccccac ttgcagttcc agatcgcgca ctgcggccag accaccatgc acctgcagcc
     2521 tggccacccc acaccgccgc ccacgcccgt gcccagcccg cacgctgcgc ccgccttggg
     2581 tgctgcgggc ctgcctggcc ccgggagcgc gctcaagggc ttggccggtg cgcaccccga
     2641 cctccgcacg ggaggcggcg gcggtggcag cggtgccggt gcgggcaaag ccaagaagtc
     2701 ggtggacaag aacagcaacg agtaccgggt acggcgggaa cgcaacaaca tcgcggtgcg
     2761 caagagccga gataaagcca aacaacgcaa cgtggagacg caacagaagg tgctggagtt
     2821 gaccagtgac aatgaccgcc tgcgcaagcg ggtggaacag ctgagccgtg aactggacac
     2881 gctgcggggc atcttccgcc agctgcctga gagctccttg gtcaaggcca tgggcaactg
     2941 cgcgtgagaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     3001 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3061 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3121 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     3181 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     3241 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     3301 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     3361 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     3421 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     3481 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     3541 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
     3601 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     3661 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     3721 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     3781 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     3841 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     3901 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     3961 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     4021 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     4081 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     4141 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     4201 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     4261 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     4321 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     4381 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     4441 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     4501 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     4561 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4621 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4681 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4741 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4801 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4861 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4921 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4981 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     5041 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     5101 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5161 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5221 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     5281 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     5341 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     5401 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     5461 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     5521 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     5581 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     5641 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     5701 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     5761 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     5821 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     5881 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     5941 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6001 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6061 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     6121 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6181 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6241 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6301 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6361 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6421 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6481 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6541 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6601 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6661 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6721 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6781 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6841 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6901 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6961 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7021 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7081 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7141 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7201 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7261 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7321 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7381 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7441 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7501 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7561 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7621 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7681 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7741 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7801 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7861 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7921 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7981 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8041 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8101 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8161 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8221 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8281 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     8341 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     8401 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     8461 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     8521 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     8581 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     8641 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     8701 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     8761 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     8821 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     8881 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     8941 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     9001 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     9061 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
     9121 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     9181 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     9241 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     9301 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     9361 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     9421 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     9481 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     9541 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     9601 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     9661 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     9721 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     9781 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     9841 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     9901 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     9961 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
    10021 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
    10081 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
    10141 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
    10201 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
    10261 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
    10321 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
    10381 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
    10441 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
    10501 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttc
//
