LOCUS       sequence-292077-       10943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of mouse Irf8 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 10943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-Irf8
FEATURES             Location/Qualifiers
     source          1..10943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    167..284
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(341..359,360..382,383..401,402..424,425..443,444..466,
                     467..485,486..508,509..527,528..550,551..569,570..592,
                     593..611)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        644..682
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     679..703
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3362..3950
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3415..3435)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3833..3844)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3953..3969)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3954..3970)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4475..4655
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4681..4698)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4687..4911
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4957..5385
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5044..5063)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5254..5275
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5394..5414)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5399..5728
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5579..5714
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5641..5660
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5776..5823
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5842..6216
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6346..6467
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6383..6402)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6437..6456
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6516..6532)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6516..6532)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6529..6551)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6540..6556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6564..6570,6571..6588,6589..6594))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6609..6630
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6747..6764)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6918..7506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6998..7017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7677..8468,8469..8537))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8300..8319
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8538..8642)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8717..8736)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8908..9287
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9289..9487
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9505..9685
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9732..9857
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10350..10583
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10768..10812
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
       61 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
      121 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      181 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      241 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      301 acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa
      361 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      421 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
      481 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      541 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      601 agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg
      661 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
      721 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt
      781 catgtgtgac cggaacggcg ggcggcggct gcggcagtgg ctgatcgaac agatcgacag
      841 cagcatgtac ccggggctga tctgggaaaa tgatgagaag accatgttcc gtatcccctg
      901 gaagcatgcc ggcaagcagg attacaatca ggaggtggat gcttccatct tcaaggcctg
      961 ggcagttttt aaagggaagt ttaaagaggg agacaaagct gaaccagcca cgtggaagac
     1021 gaggttacgc tgtgctctga acaagagccc agattttgaa gaagtgactg accggtccca
     1081 gctggacatt tctgagccat ataaagttta ccgaattgtc cccgaggaag aacaaaaatg
     1141 caagctgggc gtggcacctg caggctgcat gagcgaagtt cctgagatgg agtgtggccg
     1201 ctcagagatt gaggagctga tcaaggaacc ttctgtggat gagtacatgg gtatgaccaa
     1261 gaggagccca tccccaccag aggcctgcag gagccagatc ctccctgact ggtgggtcca
     1321 gcagcccagt gcaggcctgc cactggtgac cggatatgcc gcctatgaca cacaccattc
     1381 agctttctcc cagatggtca tcagcttcta ctacgggggc aagctggtgg gccaggccac
     1441 caccacctgc cttgaaggct gccgtctctc cctgagccag ccggggctgc ctaagttgta
     1501 tgggccggat ggcctggaac ccgtgtgctt tccgacggcc gacaccatcc ccagtgagcg
     1561 gcagaggcag gtgacccgga agctgtttgg gcacctggaa cgtggcgtgc tactgcacag
     1621 caaccgcaag ggcgtgttcg tgaagcggct gtgccagggc cgcgtgttct gcagcggcaa
     1681 cgcggtggtg tgcaagggca ggcccaacaa gctggagcgg gacgaggtgg tgcaggtctt
     1741 tgacaccaac cagttcatcc gagagctgca gcaattctac gccacccaga gccgcctacc
     1801 tgacagcagg gtggtcctgt gcttcgggga ggagtttccg gacactgtgc ccttgcgctc
     1861 caaactcatt ctggtgcagg tagagcagct gtatgccagg cagctggtgg aggaagcggg
     1921 caagagctgc ggtgctggct ccctgatgcc agccctggag gagccccagc cggaccaggc
     1981 tttccgcatg tttccggata tctgtacctc acaccagaga ccctttttta gagaaaatca
     2041 acagatcacc gtctaagaat tcatgtgtga ccggaacggc gggcggcggc tgcggcagtg
     2101 gctgatcgaa cagatcgaca gcagcatgta cccggggctg atctgggaaa atgatgagaa
     2161 gaccatgttc cgtatcccct ggaagcatgc cggcaagcag gattacaatc aggaggtgga
     2221 tgcttccatc ttcaaggcct gggcagtttt taaagggaag tttaaagagg gagacaaagc
     2281 tgaaccagcc acgtggaaga cgaggttacg ctgtgctctg aacaagagcc cagattttga
     2341 agaagtgact gaccggtccc agctggacat ttctgagcca tataaagttt accgaattgt
     2401 ccccgaggaa gaacaaaaat gcaagctggg cgtggcacct gcaggctgca tgagcgaagt
     2461 tcctgagatg gagtgtggcc gctcagagat tgaggagctg atcaaggaac cttctgtgga
     2521 tgagtacatg ggtatgacca agaggagccc atccccacca gaggcctgca ggagccagat
     2581 cctccctgac tggtgggtcc agcagcccag tgcaggcctg ccactggtga ccggatatgc
     2641 cgcctatgac acacaccatt cagctttctc ccagatggtc atcagcttct actacggggg
     2701 caagctggtg ggccaggcca ccaccacctg ccttgaaggc tgccgtctct ccctgagcca
     2761 gccggggctg cctaagttgt atgggccgga tggcctggaa cccgtgtgct ttccgacggc
     2821 cgacaccatc cccagtgagc ggcagaggca ggtgacccgg aagctgtttg ggcacctgga
     2881 acgtggcgtg ctactgcaca gcaaccgcaa gggcgtgttc gtgaagcggc tgtgccaggg
     2941 ccgcgtgttc tgcagcggca acgcggtggt gtgcaagggc aggcccaaca agctggagcg
     3001 ggacgaggtg gtgcaggtct ttgacaccaa ccagttcatc cgagagctgc agcaattcta
     3061 cgccacccag agccgcctac ctgacagcag ggtggtcctg tgcttcgggg aggagtttcc
     3121 ggacactgtg cccttgcgct ccaaactcat tctggtgcag gtagagcagc tgtatgccag
     3181 gcagctggtg gaggaagcgg gcaagagctg cggtgctggc tccctgatgc cagccctgga
     3241 ggagccccag ccggaccagg ctttccgcat gtttccggat atctgtacct cacaccagag
     3301 accctttttt agagaaaatc aacagatcac cgtctaagaa ttcgatatca agcttatcga
     3361 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     3421 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3481 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     3541 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     3601 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3661 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3721 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     3781 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3841 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3901 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     3961 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     4021 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     4081 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4141 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     4201 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     4261 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     4321 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     4381 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     4441 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     4501 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     4561 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     4621 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     4681 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     4741 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     4801 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     4861 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     4921 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     4981 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5041 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5101 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5161 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5221 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5281 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5341 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     5401 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5461 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     5521 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     5581 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5641 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     5701 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     5761 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     5821 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     5881 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     5941 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     6001 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     6061 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     6121 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     6181 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     6241 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6301 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     6361 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6421 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     6481 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6541 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6601 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6661 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6721 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6781 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6841 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6901 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6961 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7081 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7321 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7381 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7441 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7561 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7741 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7801 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7861 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7981 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8041 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8101 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8161 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8221 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8281 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8341 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8401 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8461 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8521 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8581 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8641 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     8701 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     8761 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     8821 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     8881 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     8941 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     9001 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     9061 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     9121 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     9181 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     9241 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     9301 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     9361 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     9421 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     9481 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     9541 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     9601 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     9661 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     9721 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     9781 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     9841 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     9901 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     9961 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
    10021 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
    10081 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
    10141 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
    10201 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
    10261 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    10321 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    10381 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    10441 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    10501 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    10561 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
    10621 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
    10681 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
    10741 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
    10801 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
    10861 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
    10921 aatagagtta ggcagggata ttc
//
