LOCUS       sequence-292078-        9490 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of mouse Batf3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9490)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 9490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-Batf3
FEATURES             Location/Qualifiers
     source          1..9490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        303..341
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     338..362
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      816..825
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             822..1532
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1235..1254
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1568..2156
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1621..1641)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2039..2050)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2159..2175)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2160..2176)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2681..2861
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2887..2904)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2893..3117
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3163..3591
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3250..3269)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3460..3481
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3600..3620)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3605..3934
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3785..3920
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3847..3866
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3982..4029
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4048..4422
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4552..4673
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4589..4608)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4643..4662
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4722..4738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4722..4738)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4735..4757)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4746..4762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4770..4776,4777..4794,4795..4800))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4815..4836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4953..4970)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5124..5712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5204..5223)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5883..6674,6675..6743))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6506..6525
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6744..6848)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6923..6942)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7114..7493
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7495..7693
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7711..7891
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7938..8063
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8556..8789
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8974..9018
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9316..9433
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(9490,1..18,19..41,42..60,61..83,84..102,103..125,
                     126..144,145..167,168..186,187..209,210..228,229..251,
                     252..270)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
       61 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
      121 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
      181 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
      241 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc
      301 gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga
      361 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      421 gcctccgcgg ccccgaattc atgtcgcaag ggccccccgc ggtcagcgtg ctgcagagaa
      481 gcgtggatgc gcccgggaac cagccgcaga gccccaagga cgatgacagg aaagttcgaa
      541 ggagagagaa aaaccgggtt gcagctcaga ggagccggaa gaagcagacc cagaaggctg
      601 acaagctcca cgaggagcac gagagcctgg agcaggagaa ctctgtgctg cgcagggaga
      661 tttcgaagct gaaggaggag ctgcgtcacc tgagcgaggt gctgaaggag cacgagaaga
      721 tgtgcccgct gctgctgtgt cctatgaact ttgtgcagct tcggtcagac cccgtggcca
      781 gctgtctacc acgatgagaa ttcggcttac tagtcgccac catggtgagc aagggcgagg
      841 agaataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg
      901 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggctttc
      961 agaccgctaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     1021 cccctcagtt cacctacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     1081 acttcaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     1141 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     1201 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     1261 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     1321 agatgaggct gaagctgaag gacggcggcc actacacctc cgaggtcaag accacctaca
     1381 aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt cggcatcaag ttggacatca
     1441 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     1501 ccaccggcgg catggacgag ctgtacaagt aaagatctaa gccgaattcg atatcaagct
     1561 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1621 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     1681 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1741 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     1801 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     1861 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     1921 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     1981 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2041 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2101 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     2161 ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     2221 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     2281 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     2341 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     2401 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     2461 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     2521 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     2581 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     2641 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     2701 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     2761 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     2821 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     2881 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     2941 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     3001 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3061 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3121 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     3181 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3241 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3301 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3361 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3421 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3481 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3541 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     3601 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3661 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3721 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3781 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3841 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     3901 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     3961 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     4021 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     4081 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     4141 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     4201 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     4261 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     4321 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     4381 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     4441 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     4501 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     4561 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4621 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     4681 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     4741 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4801 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4861 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     4921 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4981 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5041 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5101 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5161 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5221 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5281 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5341 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5401 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5461 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5521 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5581 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5641 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5701 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5761 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5821 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5881 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5941 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6001 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6061 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6121 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6181 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6241 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6301 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6361 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6421 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6481 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6541 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6601 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6661 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6721 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6781 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6841 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     6901 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     6961 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     7021 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     7081 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     7141 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     7201 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     7261 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     7321 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     7381 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     7441 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     7501 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     7561 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     7621 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     7681 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     7741 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7801 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7861 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     7921 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     7981 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8041 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8101 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8161 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8221 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8281 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8341 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8401 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     8461 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     8521 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     8581 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     8641 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     8701 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     8761 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     8821 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     8881 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     8941 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     9001 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     9061 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     9121 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     9181 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     9241 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
     9301 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     9361 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     9421 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt tagatctcga
     9481 gtttaccact
//
