LOCUS       sequence-292148-       12316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration of SEC7-DuDre as an additional copy in genome, use 
            auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late
            golgi/early endosome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12316)
  AUTHORS   Zhu J, Zhang ZT, Tang SW, Zhao BS, Li H, Song JZ, Li D, Xie Z
  TITLE     A Validated Set of Fluorescent-Protein-Based Markers for Major 
            Organelles in Yeast (Saccharomyces cerevisiae).
  JOURNAL   MBio. 2019 Sep 3;10(5). pii: mBio.01691-19. doi: 
            10.1128/mBio.01691-19.
  PUBMED    31481383
REFERENCE   2  (bases 1 to 12316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-09-3"; volume: "10(5). pii: mBio.01691-19. doi"; pages: 
            " 10.1128/mBio.01691-19"
COMMENT     Sequence Label: ZJOM40
FEATURES             Location/Qualifiers
     source          1..12316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      8592..8779
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8647..8671)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    8831..8864
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    9867..9900
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(9953..9972)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9954..9972)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10059..10076)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10230..10818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10310..10329)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10989..11780,11781..11849))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11612..11631
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11850..11954)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12022..12040
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12078..12100)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12200..12219
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(12300..12316,1..2)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     join(12300..12316,1)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gaacgcggcc gccagctgaa aatgttgaga taattgttgg gattccattg ttgataaagg
       61 ctataatatt aggtatacag aatatactag aagttctcct cgagggtcag gaatccccat
      121 aatggaatct atatttctat ataccaatat tacgattatt cctcattctg ttttatatgt
      181 ttcattatcc tattacatta tcaatccttg cacttcagct tcctctaact tcgatgacag
      241 cttctcataa cttatgtcat catcttaaca ccgtatatga taatatattg ataatataac
      301 tattagttga tagacgatag tggattttta ttccaacact atgtgtgtat tgtgtgtgcg
      361 tgtaatataa tcatttctag tcatctctag tcaaagtaac ttataaaagc ctaaaaaagg
      421 aggatttaac atgaattgtc ggtcctatag tgtagtggtt atcactttcg gttttgatcc
      481 ggacaacccc ggttcgaatc cgggtaggac ctctttttat ttttactcta acaaatcaac
      541 attgatattt tttgttgatg tgttagagtg gagccaacga tgctcttaag atatacactt
      601 tagtactttt gaagctctta cagttttttg atgtcggaga aggcttatac atattgttat
      661 tttccctcta caggaaagaa ggtgaacgtt tagtttttat caaggcgccg agtaaatttt
      721 ataatatcga ggcgagggaa aaacaaagca acgagaaagg aagctcaaag ttgggattta
      781 catcatttct ttaaaaagca tcattggctt ctctccaatt gttcaataaa cgcagactaa
      841 tagaaattga agtcattttc cggagaaata taaaatattt tctgcaacta agctatactc
      901 gatttctttt aagtttctcc agaaccaata aaaagtaata tcttaaactg tttctttctc
      961 attttttccc cgaattccat taaagaagat aagaaacaag tctaagtgct actatatctg
     1021 atgtctgaac agaattcagt agtaaatgct gaaaaagggg atggcgaaat atcttcaaat
     1081 gtagagactg cttcttcagt caatccgtct gtgaaaccac agaatgctat taaagaagaa
     1141 gctaaagaga caaatggtga agatcaaaaa tgcaaagggc cagaaaacgc tggcagtacg
     1201 gcagaaacaa aagaaacaag taacgacgct accaacggaa tgaaaacgcc cgaagaaacg
     1261 gaagatacaa atgacaaacg ccatgacgat gaaggtgaag atggagatga agatgaagat
     1321 gaagatgaag atgaagatga agacaatggg gacgaggatg atgaggacgt ggatagcagt
     1381 agttccgaaa ccagttccga agacggggaa gattctgaat cagtctcagg ggaaagtact
     1441 gagagtagct ctggagaaga tgaagaatct gatgaaagcg atggaaatac atcaaacagc
     1501 tcctctggtg atgaaagcgg gtcagaagaa gaagaagaag aagaagaaga agaagaagaa
     1561 gaggaaaatg ctggcgaacc agctatcgct catcaagata gtgttcccac caacgattct
     1621 actgccccaa ggtctaccca tacgaggaac atatcactat cttcaaatgg ttcgaacaca
     1681 aactcaacca taattttagt gaaaactacg ttggagacaa ttttaaatga taaggacatt
     1741 aaaaagaatt cgaatgctca gaaggctatc gaaaggacat tacaaaaatt taaggaattt
     1801 gatccgcaaa ccacgaataa cccacattac gtggattcaa tactagtatt cgaagcacta
     1861 agggcaagct gtcgtaccaa atcctccaaa gttcaaagtt tggctttaga ttgcctgtca
     1921 aaattgtttt cttttagatc gctagacgag accctgttag tgaatccacc cgattcttta
     1981 gcctctaatg atcaacgaca agatgctgcc gatggaataa cgccgcctcc aaaacaaaaa
     2041 attatagacg ctgcaattga tactatttca gactgttttc aaggtgaagg cactgatgac
     2101 cgtgtggaac tacaaatcgt tagagcttta tctagttgca ttttagaaga ggattcaagt
     2161 tctttatgcc acggtgcttc cttgctaaag gctatcagaa caatctacaa cgttttcgtc
     2221 ttttcgttga acccatccaa tcaaggtatt gcacaggcga ccttgacaca aattattagt
     2281 tcggtgtatg ataaaatcga tctcaaacaa agtacctctt cagcagtatc cttatcgaca
     2341 aaaaatcatc aacaacaatc cgccatagaa ctttccgagg cttctgaaaa tgcagaaacg
     2401 ccggctcctt taactctgga aaatatggat aagttgaatg acgatgagga aagactaatg
     2461 gacgctcaac agcctgattc tatcgccata actaaccaag atctagctgt taaagatgcg
     2521 ttcttagtgt ttagagtcat ggcgaaaata tgtgctaaac ctttggaaac agaactcgac
     2581 atgaggtcac atgccgtcag gtcaaagctt ttatctcttc acatcattta ctctattatc
     2641 aaagatcata ttgacgtatt cctttcccac aacatttttc taccaggaaa ggagcgtgtg
     2701 tgctttatcg attcaattag acaatattta cgtcttgttt tatcaaggaa tgctgcctcg
     2761 cctctagctc cagttttcga ggttacttta gaaattatgt ggctattgat tgctaatttg
     2821 agagcagatt tcgtgaagga aattccagtt tttttaacag aaatctactt ccccatttca
     2881 gaattaacca cttccacctc ccaacaaaag agatattttt taagtgttat tcaacgaatt
     2941 tgtaacgacc caagaacttt agttgaattt tacttgaatt atgattgtaa ccctggaatg
     3001 ccaaatgtaa tggaaataac tgttgattat ttgacaagat tggctttaac tagggtggaa
     3061 attactcaga ctcaaagatc atactacgat gaacaaatat caaaatccct gtccacctac
     3121 aatttctcac aattgcccct tttgacttct tccaacttgt cttctagtcc tgatgttggt
     3181 caagtcaatt tactttttcc acttgatttt gctctcaaaa tggtgtcact aaattgcatt
     3241 gtgtcagttt tgcgttcctt aagctcatgg gctcacaaag ctttaaatcc aaacacacac
     3301 actgctaata aggtattact aaacacaaca tcttcagctc gtcaagaatc tagatcatct
     3361 ttgagtaacg acgtaaggtc ttctattatg acaagtaatg atgacttcaa accaacatat
     3421 gaagacgaag aaagcagatc attgagcagt caaaacattg acgcagacga ccccacacaa
     3481 tttgaaaatt tgaaactaag gaaaactgct ttatcggaat gtattgctat tttcaacaat
     3541 aaacccaaga aagctattcc agtacttatt aaaaaaggat ttttgaaaga tgattctcca
     3601 atttccattg ccaagtggtt attagaaaca gaaggcctcg acatggccgc tgttggggat
     3661 tatctaggcg aaggagacga caagaacatc gctataatgc acgcatttgt tgatgagttt
     3721 gacttcactg gtatgtccat tgttgacgca ttaaggtcat ttttacaaag tttcagattg
     3781 cctggagaag gtcaaaaaat tgacagattc atgctgaaat ttgcggaaag atttgtggac
     3841 caaaaccccg gagtcttttc aaaggcggat actgcatatg tgctttcgta ttctttgatc
     3901 atgttgaata ctgatttaca ttcgtcacaa atcaaaaata aaatgtcttt acaagagttt
     3961 ttagaaaaca acgaaggtat tgacaacgga agagatttac caagagactt cttggaaggt
     4021 ttgttcaacg aaattgctaa caatgaaatc aagttaattt ctgaacagca tcaggcaatg
     4081 ctttcaggtg ataccaatct tgtccaacaa cagcaatctg ctttcaactt ctttaattct
     4141 cgtgatttga caagagaggc atataatcaa gtctcaaaag aaatttcatc taaaacggaa
     4201 ttagtcttta agaatttaaa caaaaataaa ggaggcccag acgtctatta tgctgcttcc
     4261 cacgttgagc atgttaaatc aattttcgag acactatgga tgtccttttt agcagctcta
     4321 acccccccat ttaaggatta tgatgacatt gacacaacca ataagtgttt agaaggcttg
     4381 aaaatatcaa ttaaaattgc ttctactttt agaatcaatg atgctagaac ctcctttgta
     4441 ggtgcgttag tccaattttg taaccttcaa aaccttgaag aaatcaaagt taaaaatgtc
     4501 aatgcaatgg taattcttct tgaagtcgcg ttatcagaag gaaattactt ggagggatcg
     4561 tggaaggaca ttttgctggt cgtgtctcaa atggaaagac tacaattgat atccaaaggt
     4621 atcgacagag atacggttcc agatgttgca caagctcgtg ttgcaaaccc tagagtttct
     4681 tacgaatcat caagatccaa taatacatct ttctttgatg tttggggcaa gaaggcaact
     4741 cccacagaat tggctcaaga aaaacaccat aatcaaacct tatcacccga aatctctaaa
     4801 ttcatttcct ccagtgaatt agtcgttttg atggacaata tatttaccaa aagttccgag
     4861 ttatcaggta atgctatcgt tgattttatc aaagctttaa ccgctgtatc tttagaagaa
     4921 attgaatcat ctgaaaatgc ttccacacca agaatgtttt ccttgcaaaa aatggtcgat
     4981 gtatgttact acaatatgga tcgtatcaaa ctagaatgga cgccgctttg ggctgttatg
     5041 ggaaaagctt tcaacaagat tgctacaaac tctaacttag cagtagtatt tttcgctatc
     5101 gattccctgc gtcaattgtc tatgagattt ttagatattg aggaattatc aggttttgaa
     5161 tttcaacatg attttttaaa accttttgaa tacacggttc aaaatagtgg caacactgaa
     5221 gttcaagaaa tgattattga gtgttttagg aacttcatat tgacaaaatc ggaaagtatc
     5281 aaatctggct ggaagcctat tcttgaatcc ttacaatata cggctcgctc aagcactgaa
     5341 tccattgtat taaagacgca attgctggtt agcaatgata ttgtgacaaa tcacttcgaa
     5401 aacgtattct ctcaagaaga tgccttttct gagttagttg gtgtcttcag agaaatcacc
     5461 aaaaataaga gattccaaaa gctatctcta catgctttgg agtctctaag aaagatgact
     5521 cagaacgtcg cagacatctg tttttacaat gaaaataaga ctgaagaaga aagaaaacat
     5581 aacgatgctt tacttcgtgg gaaagacata ttccaggatg tgtggttccc tatgttattc
     5641 tgcttcaatg atacgatcat gacagctgaa gacttagaag ttagatcacg tgcattaaac
     5701 tatatgttcg atgccctagt ggcatatggt ggtaaattca atgatgattt ctgggaaaag
     5761 atttgtaaga agttactatt tcctattttc ggtgtattat ccaaacattg ggaagtcaac
     5821 caattcaata gtcatgatga tttaagtgtt tggctttcga ccacgttaat tcaagcctta
     5881 aggaacttga ttgccctttt tacgcattac tttgaatcgt tgaacagaat gctcgatgga
     5941 tttttgggtc tgctggtatc ctgtatttgt caagaaaatg acactattgc tagaattggg
     6001 agatcctgcc tgcaacaatt gatattgcaa aacgtatcta aattcaatga gtaccattgg
     6061 aatcaaatag gtgacgtatt cgataaattg tttgatttaa ccactgctaa tgaattgttc
     6121 gattatgatc cgttacagca aggaagaaaa tcatctgtat ctcatcacca aacaactaat
     6181 gataccagcc aacactcaga tgatgatagc aacgacagaa gggagaacga ttctaatatc
     6241 agtgagacgg tcgaaagagc gcatcaagaa gaatccagcg aagacgttgg tggtgacatg
     6301 gttgaaacac tcaatgggca aactaagctt aataatggca attccgttcc gacggtaaag
     6361 gacgaattga atccaaagcc cgctagtcta agtatcccca agaaaactaa gcacatgaaa
     6421 cgaaacgaaa gtaacgaaga tatacgtagg agaataaaca tcaagaattc tattgttgtc
     6481 aaatgtgttt tacagctttt gatgatagag ctgctcaacg aattattcga gaacgaagat
     6541 tttgcccact gtatccctta caaggaagcc attagaatta caagattgtt ggagaaatcg
     6601 tacgaatttt ctcgtgactt taatgaagat tatgggttaa gaacaagact agtagaggct
     6661 cgtgtagttg ataaaatacc aaacctgttg aaacaagaaa cgagtgctgc tgcagttctc
     6721 cttgacatta tgtttcagtt gtatctgaac gatgatgaga agaaggctga tttaataact
     6781 cgtctgatca ccatttgcat ccaggtcgta gagggttatg tttctttgga tgatagaacc
     6841 atggaacgta gtatcaatgc ctggcgttca gtgatagttg aaatattaca aggttactac
     6901 gaatttgatg atgaagactt tagactatac tgtcctgcta tgtatgctct ggtgattcaa
     6961 atattagata agagcgttcc aacggagttg agacatgcta taaaacaatt tctaagcaga
     7021 gttggtgaat tatacctttc tactgatgct tcgtacgctg caggtcgtgg gattcctggg
     7081 ttaattaaca tcgctagcat ggtttccaaa ggtgaagaga acaatatggc tgtcatcaag
     7141 cccttcatga gattcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     7201 gagggcgagg gcgagggcaa gccctacgag ggcacccaga ccgccaagct gcaggtgacc
     7261 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc
     7321 aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag
     7381 ggcttcaagt gggagagagt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     7441 gactcctccc tgcaggacgg caccttcatc taccacgtga agttcatcgg cgtgaacttc
     7501 ccctccgacg gccccgtaat gcagaagaag actctgggct gggagccctc caccgagaga
     7561 ctgtacccca gagacggcgt gctgaagggc gagatccaca aggcgctgaa gctgaagggc
     7621 ggcggccact acctggtgga gttcaagtca atctacatgg ccaagaagcc cgtgaagctg
     7681 cccggctact actacgtgga ctccaagctg gacatcacct cccacaacga ggactacacc
     7741 gtggtggagc agtacgagag agccgaggcc agacaccacc tgttcttaat tccagttcca
     7801 acgcgtgatc ttgagaagcc attcaacgtc atcaagccct tcatgagatt caaggtgcac
     7861 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggcaagccc
     7921 tacgagggca cccagaccgc caagctgcag gtgaccaagg gcggccccct gcccttcgcc
     7981 tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc
     8041 gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga gagagtgatg
     8101 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcacc
     8161 ttcatctacc acgtgaagtt catcggcgtg aacttcccct ccgacggccc cgtaatgcag
     8221 aagaagactc tgggctggga gccctccacc gagagactgt accccagaga cggcgtgctg
     8281 aagggcgaga tccacaaggc gctgaagctg aagggcggcg gccactacct ggtggagttc
     8341 aagtcaatct acatggccaa gaagcccgtg aagctgcccg gctactacta cgtggactcc
     8401 aagctggaca tcacctccca caacgaggac tacaccgtgg tggagcagta cgagagagcc
     8461 gaggccagac accacctgtt cttaattgtc gttctgaaca gagctgagac tccattgcca
     8521 ttggatccaa ccggtaaggt caaggctgag ctggatacta ggcctaggtg aggcgcgcca
     8581 cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa
     8641 aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct
     8701 tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag gtcgctctta
     8761 ttgaccacac ctctaccggc agatccgcta gggataacag ggtaatatct gcaggtcgac
     8821 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc tagagcggcc
     8881 gcggaacgat cattcactat atatatatca atttatatat acgtatgtgt aattgaagaa
     8941 agatacgttt tttcctctat tgagaggcct gctggatgaa tagctttacc ttttctaaat
     9001 ccttgatacc atcagtctct actcctccac ttacatcgac accgatcgca tttggtaaca
     9061 tattaatggc aacagaaacg ttatcaggat tcaatccacc agcgataatg aattttatct
     9121 cgggatgact tgcagaccaa ctggaaattg cactccaatt caatttctca ccagtgccac
     9181 cttcaccaga atcgaacaac gtcagcacat tgtctacgtg ttcatacagg tccagtagta
     9241 attcacaatc ctgtgggaac tggaacctct taatgattgg aattgaagat gggatcaaag
     9301 atctgtattc tttaatatct tcatctccat gtaattgtat cacatctaga ttatattcgt
     9361 ggtacagttg aaggacatca tcaacggact gatttctaaa caccccgact agtttagtac
     9421 ctttcacgtt ctcttgttgg tgaactgcag ttgaaatacc tttcgcaaca gatgagtcaa
     9481 tggttctttt cctaccggga acacaaatga tacctaagta atcagcacca tcatccacag
     9541 cagtctttgc agcttcaacg gtttgcaaac cacacacttt aacgagcatc acagtagatt
     9601 tcgcaagagg gtagctcgct cagagtaccc aagtaaatga ttagtaaact gatgtttgat
     9661 agttcaattt ttcaatgaaa taaccttata ttaaaattga tattactatt atacaaaaat
     9721 aaagaataaa ggatttgagt ttatacataa aataccatta ttatttgttc agtgagagat
     9781 actggggtat atgggatgtg tgtagtgata ccatgcaatc atgtatcaaa catgggcccg
     9841 gtaccgagct ctcgagaacc cttaatataa cttcgtataa tgtatgctat acgaagttat
     9901 taggtgatat cagatccact agtggcctat gcggccgcgg atctgccggt ctccctatag
     9961 tgagtcgtat taatttcgat aagccaggtt aacctgcatt aatgaatcgg ccaacgcgcg
    10021 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
    10081 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    10141 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    10201 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    10261 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    10321 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    10381 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    10441 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    10501 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    10561 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    10621 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    10681 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    10741 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10801 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10861 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10921 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10981 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    11041 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    11101 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    11161 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    11221 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    11281 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    11341 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    11401 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    11461 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    11521 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    11581 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    11641 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    11701 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    11761 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11821 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11881 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11941 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
    12001 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    12061 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    12121 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    12181 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatggac
    12241 atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat acacatacga
    12301 tttaggtgac actata
//
