LOCUS       sequence-292192-       10446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a mutant (Y951F) VEGFR2-mCherry fusion protein in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Pietila I, Van Mourik D, Tamelander A, Kriz V, Claesson-Welsh L, 
            Tengholm A, Welsh M
  TITLE     Temporal Dynamics of VEGFA-Induced VEGFR2/FAK Co-Localization Depend
            on SHB.
  JOURNAL   Cells. 2019 Dec 15;8(12). pii: cells8121645. doi: 
            10.3390/cells8121645.
  PUBMED    31847469
REFERENCE   2  (bases 1 to 10446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2019-12-15"; volume: "8(12). pii: cells8121645. doi"; pages: 
            " 10.3390/cells8121645"
COMMENT     Sequence Label: pcDNA3.1-Y951F-VEGFR2-mCherry
FEATURES             Location/Qualifiers
     source          1..10446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5112..5822
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5257..5275)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5525..5544
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5868..5885)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5874..6098
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6144..6572
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6231..6250)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6441..6462
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6581..6601)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6586..6915
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6766..6901
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6828..6847
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6964..7989
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6964..7989
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8119..8240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8156..8175)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8210..8229
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8289..8305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8289..8305)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8302..8324)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8313..8329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8337..8343,8344..8361,8362..8367))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8382..8403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8520..8537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8691..9279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8771..8790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9450..10241,10242..10310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10073..10092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10311..10415)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      961 agatcgaggt cgacgcgcca tggagctcca tggcgcgtcg acgcgccatg gagaattcca
     1021 gcaaggtgct gctggccgtc gccctgtggc tctgcgtgga gacccgggcc gcctctgtgg
     1081 gtttgcctag tgtttctctt gatctgccca ggctcagcat acaaaaagac atacttacaa
     1141 ttaaggctaa tacaactctt caaattactt gcaggggaca gagggacttg gactggcttt
     1201 ggcccaataa tcagagtggc agtgagcaaa gggtggaggt gactgagtgc agcgatggcc
     1261 tcttctgtaa gacactcaca attccaaaag tgatcggaaa tgacactgga gcctacaagt
     1321 gcttctaccg ggaaactgac ttggcctcgg tcatttatgt ctatgttcaa gattacagat
     1381 ctccatttat tgcttctgtt agtgaccaac atggagtcgt gtacattact gagaacaaaa
     1441 acaaaactgt ggtgattcca tgtctcgggt ccatttcaaa tctcaacgtg tcactttgtg
     1501 caagataccc agaaaagaga tttgttcctg atggtaacag aatttcctgg gacagcaaga
     1561 agggctttac tattcccagc tacatgatca gctatgctgg catggtcttc tgtgaagcaa
     1621 aaattaatga tgaaagttac cagtctatta tgtacatagt tgtcgttgta gggtatagga
     1681 tttatgatgt ggttctgagt ccgtctcatg gaattgaact atctgttgga gaaaagcttg
     1741 tcttaaattg tacagcaaga actgaactaa atgtggggat tgacttcaac tgggaatacc
     1801 cttcttcgaa gcatcagcat aagaaacttg taaaccgaga cctaaaaacc cagtctggga
     1861 gtgagatgaa gaaatttttg agcaccttaa ctatagatgg tgtaacccgg agtgaccaag
     1921 gattgtacac ctgtgcagca tccagtgggc tgatgaccaa gaagaacagc acatttgtca
     1981 gggtccatga aaaacctttt gttgcttttg gaagtggcat ggaatctctg gtggaagcca
     2041 cggtggggga gcgtgtcaga atccctgcga agtaccttgg ttacccaccc ccagaaataa
     2101 aatggtataa aaatggaata ccccttgagt ccaatcacac aattaaagcg gggcatgtac
     2161 tgacgattat ggaagtgagt gaaagagaca caggaaatta cactgtcatc cttaccaatc
     2221 ccatttcaaa ggagaagcag agccatgtgg tctctctggt tgtgtatgtc ccaccccaga
     2281 ttggtgagaa atctctaatc tctcctgtgg attcctacca gtacggcacc actcaaacgc
     2341 tgacatgtac ggtctatgcc attcctcccc cgcatcacat ccactggtat tggcagttgg
     2401 aggaagagtg cgccaacgag cccagccaag ctgtctcagt gacaaaccca tacccttgtg
     2461 aagaatggag aagtgtggag gacttccagg gaggaaataa aattgaagtt aataaaaatc
     2521 aatttgctct aattgaagga aaaaacaaaa ctgtaagtac ccttgttatc caagcggcaa
     2581 atgtgtcagc tttgtacaaa tgtgaagcgg tcaacaaagt cgggagagga gagagggtga
     2641 tctccttcca cgtgaccagg ggtcctgaaa ttactttgca acctgacatg cagcccactg
     2701 agcaggagag cgtgtctttg tggtgcactg cagacagatc tacgtttgag aacctcacat
     2761 ggtacaagct tggcccacag cctctgccaa tccatgtggg agagttgccc acacctgttt
     2821 gcaagaactt ggatactctt tggaaattga atgccaccat gttctctaat agcacaaatg
     2881 acattttgat catggagctt aagaatgcat ccttgcagga ccaaggagac tatgtctgcc
     2941 ttgctcaaga caggaagacc aagaaaagac attgcgtggt caggcagctc acagtcctag
     3001 agcgtgtggc acccacgatc acaggaaacc tggagaatca gacgacaagt attggggaaa
     3061 gcatcgaagt ctcatgcacg gcatctggga atccccctcc acagatcatg tggtttaaag
     3121 ataatgagac ccttgtagaa gactcaggca ttgtattgaa ggatgggaac cggaacctca
     3181 ctatccgcag agtgaggaag gaggacgaag gcctctacac ctgccaggca tgcagtgttc
     3241 ttggctgtgc aaaagtggag gcatttttca taatagaagg tgcccaggaa aagacgaact
     3301 tggaaatcat tattctagta ggcacggcgg tgattgccat gttcttctgg ctacttcttg
     3361 tcatcatcct acggaccgtt aagcgggcca atggagggga actgaagaca ggctacttgt
     3421 ccatcgtcat ggatccagat gaactcccat tggatgaaca ttgtgaacga ctgccttatg
     3481 atgccagcaa atgggaattc cccagagacc ggctgaagct aggtaagcct cttggccgtg
     3541 gtgcctttgg ccaagtgatt gaagcagatg cctttggaat tgacaagaca gcaacttgca
     3601 ggacagtagc agtcaaaatg ttgaaagaag gagcaacaca cagtgagcat cgagctctca
     3661 tgtctgaact caagatcctc attcatattg gtcaccatct caatgtggtc aaccttctag
     3721 gtgcctgtac caagccagga gggccactca tggtgattgt ggaattctgc aaatttggaa
     3781 acctgtccac ttacctgagg agcaagagaa atgaatttgt cccctacaag accaaagggg
     3841 cacgattccg tcaagggaaa gacttcgttg gagcaatccc tgtggatctg aaacggcgct
     3901 tggacagcat caccagtagc cagagctcag ccagctctgg atttgtggag gagaagtccc
     3961 tcagtgatgt agaagaagag gaagctcctg aagatctgta taaggacttc ctgaccttgg
     4021 agcatctcat ctgttacagc ttccaagtgg ctaagggcat ggagttcttg gcatcgcgaa
     4081 agtgtatcca cagggacctg gcggcacgaa atatcctctt atcggagaag aacgtggtta
     4141 aaatctgtga ctttggcttg gcccgggata tttataaaga tccagattat gtcagaaaag
     4201 gagatgctcg cctccctttg aaatggatgg ccccagaaac aatttttgac agagtgtaca
     4261 caatccagag tgacgtctgg tcttttggtg ttttgctgtg ggaaatattt tccttaggtg
     4321 cttctccata tcctggggta aagattgatg aagaattttg taggcgattg aaagaaggaa
     4381 ctagaatgag ggcccctgat tatactacac cagaaatgta ccagaccatg ctggactgct
     4441 ggcacgggga gcccagtcag agacccacgt tttcagagtt ggtggaacat ttgggaaatc
     4501 tcttgcaagc taatgctcag caggatggca aagactacat tgttcttccg atatcagaga
     4561 ctttgagcat ggaagaggat tctggactct ctctgcctac ctcacctgtt tcctgtatgg
     4621 aggaggagga agtatgtgac cccaaattcc attatgacaa cacagcagga atcagtcagt
     4681 atctgcagaa cagtaagcga aagagccggc ctgtgagtgt aaaaacattt gaagatatcc
     4741 cgttagaaga accagaagta aaagtaatcc cagatgacaa ccagacggac agtggtatgg
     4801 ttcttgcctc agaagagctg aaaactttgg aagacagaac caaattatct ccatcttttg
     4861 gtggaatggt gcccagcaaa agcagggagt ctgtggcatc tgaaggctca aaccagacaa
     4921 gcggctacca gtccggatat cactccgatg acacagacac caccgtgtac tccagtgagg
     4981 aagcagaact tttaaagctg atagagattg gagtgcaaac cggtagcaca gcccagattc
     5041 tccagcctga ctcggggacc acactgagct ctcctcctgt tggatccggt gggagtggag
     5101 gtgggagtgg aatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     5161 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     5221 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     5281 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     5341 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     5401 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     5461 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     5521 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     5581 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     5641 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     5701 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     5761 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     5821 aaagcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     5881 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5941 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     6001 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     6061 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     6121 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6181 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6241 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6301 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6361 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6421 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6481 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6541 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     6601 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6661 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6721 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     6781 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6841 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6901 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     6961 gtgatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     7021 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     7081 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     7141 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     7201 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     7261 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     7321 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     7381 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     7441 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     7501 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     7561 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     7621 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     7681 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     7741 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     7801 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     7861 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     7921 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     7981 aaggaatagc acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     8041 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     8101 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8161 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8221 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     8281 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8341 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8401 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8461 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8521 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8581 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8641 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8701 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8761 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8821 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8881 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8941 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9001 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9061 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9121 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9241 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9301 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9361 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9421 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9481 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9541 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9601 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9661 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9721 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9781 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9841 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9901 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9961 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10021 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10081 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10141 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10201 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10261 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10321 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10381 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10441 gacgtc
//
