LOCUS       sequence-292199-       11572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of mouse Bcl11b in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11572)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 11572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-Bcl11b
FEATURES             Location/Qualifiers
     source          1..11572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(1..19,20..42,43..61,62..84,85..103,104..126,127..145,
                     146..168,169..187,188..210,211..229,230..252,253..271)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        304..342
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     339..363
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2899..2908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2905..3615
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3318..3337
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3651..4239
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3704..3724)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4122..4133)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4242..4258)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4243..4259)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4764..4944
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4970..4987)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4976..5200
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5246..5674
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5333..5352)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5543..5564
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5683..5703)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5688..6017
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5868..6003
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5930..5949
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6065..6112
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6131..6505
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6635..6756
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6672..6691)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6726..6745
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6805..6821)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6805..6821)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6818..6840)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6829..6845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6853..6859,6860..6877,6878..6883))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6898..6919
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7036..7053)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7207..7795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7287..7306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7966..8757,8758..8826))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8589..8608
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8827..8931)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9006..9025)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9197..9576
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9578..9776
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9794..9974
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10021..10146
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10639..10872
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11057..11101
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11399..11516
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
       61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      181 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      241 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt
      301 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
      361 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      421 agcctccgcg gccccgaatt catgtcccgc cgcaaacagg gcaacccgca gcacttgtcc
      481 cagagggaac tcatcacgcc agaggctgac catgtggagg ctaccatcct cgaggaagac
      541 gagggtctgg agatagagga gcctagcagc ctggggctga tggtgggagg ccccgaccct
      601 gatctactca cctgtggcca gtgtcagatg aacttcccgc tgggggacat cctggttttt
      661 atagagcaca agaagaaaca gtgtggaggc ctgggcccct gctacgacaa ggtcctggac
      721 aagagcagtc cacctccctc ctctcgctct gagctcagga gagtatctga gccagtggag
      781 atcgggatcc aggtcacccc tgatgaagat gaccacctac tgtcacccac gaaaggcatc
      841 tgtcccaagc aggagaacat tgcaggtaaa gatgagcctt ccagctacat ttgcacaaca
      901 tgcaagcagc ccttcaacag cgcctggttc ctgctgcagc acgcacagaa cacgcatggc
      961 ttccgaatct acctggagcc tgggccggcc agcacctcgc tcacgcccag gctcaccatc
     1021 ccgccaccgc tcgggccgga gaccgtggcg cagtccccac tcatgaattt cctgggggac
     1081 agcaatcctt tcaacctgct gcgcatgacg ggccccatcc tgcgggacca ccctggcttc
     1141 ggtgagggcc gcttgccagg tacgccaccg ctcttcagcc caccgccacg ccatcacttg
     1201 gacccacacc gcctcagtgc agaggagatg gggctcgtgg cccagcaccc cagtgccttc
     1261 gaccgagtca tgcgcctgaa ccccatggcc atagactctc ctgccatgga cttctcccgg
     1321 cggctgcgag aactggccgg caacagctcc acgccgccgc ccgtgtcccc aggccgtggc
     1381 aaccctatgc accggctgct gaaccctttc cagcccagtc ccaagtcccc gttcctcagc
     1441 acgccaccgc tgccacccat gcctgcgggc acaccgccac cgcagccgcc tgccaagagc
     1501 aagtcctgtg agttctgcgg caagaccttc aagttccaga gcaatctcat cgtgcaccgg
     1561 cgcagccaca cgggcgagaa gccctacaag tgccagctgt gcgaccatgc gtgctcgcag
     1621 gcgagcaagc tcaagcgcca catgaagacg cacatgcaca aggcgggctc tctggctggc
     1681 cgctcagacg acgggctctc agctgccagc tcccctgagc cgggcaccag cgagctgcca
     1741 ggtgacctga aagcggccga tggcgacttc cgccaccatg agagcgaccc atctctgggc
     1801 cccgagcctg aggacgacga ggacgaggag gaggaagaag aggagctgct gctggagaac
     1861 gagagccggc ctgagtcgag cttcagcatg gactcggagc tgggccgtgg ccgcgagaac
     1921 ggaggtggtg tgccaccggg ggtggcgggc gcaggggctg cagctgcggc tctggcggat
     1981 gagaaggctc tggccctggg caaggtgatg gaggacgcag ggctgggcgc actgccgcag
     2041 tatggggaga agcggggcgc cttcctgaag cgtgcaggcg acacgggtga tgccggagct
     2101 gttggctgtg gggacgcggg tgcaccgggt gcagtgaacg ggcgcggcgg ggccttcgcg
     2161 ccaggcgcag agccctttcc agctctcttc ccacgcaagc cagcaccgct gcccagccct
     2221 gggctcggtg gtcccgcgct gcacgcggcc aagcgcatca aggtggagaa agacctggag
     2281 ctgccacctg ccgccctcat cccatctgag aacgtgtact cgcagtggct cgtgggctac
     2341 gcagcatcgc gccacttcat gaaggaccca ttcctgggct tcacggatgc gcgccagtcg
     2401 cctttcgcca catcgtcgga acattcctct gagaacggca gcctgcgctt ctcaacgcca
     2461 cccggggacc tgctggacgg cgggctgtcc gggcgcagtg gcacggcgag cgggggcagc
     2521 acacctcacc tgggtggtcc gggtcctggg aggccgagct ccaaggaggg ccgccgcagc
     2581 gacacatgtg agtactgcgg caaggtcttc aagaactgta gcaacctgac ggtgcaccgg
     2641 aggagccaca ccggcgagcg gccttacaag tgcgagctgt gcaactacgc gtgtgcgcag
     2701 agcagcaagc tcacgcgcca catgaagacg cacgggcaga tcggcaagga ggtgtaccgc
     2761 tgcgacatct gccagatgcc cttcagcgtc tacagcaccc tggagaaaca catgaaaaag
     2821 tggcacggtg aacacttgct gactaatgat gtcaaaatcg agcaggctga gaggagctaa
     2881 gaattcggct tactagtcgc caccatggtg agcaagggcg aggagaataa catggccatc
     2941 atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc
     3001 gagatcgagg gcgagggcga gggccgcccc tacgagggct ttcagaccgc taagctgaag
     3061 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcacctac
     3121 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttcaa gctgtccttc
     3181 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     3241 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     3301 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     3361 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagatgag gctgaagctg
     3421 aaggacggcg gccactacac ctccgaggtc aagaccacct acaaggccaa gaagcccgtg
     3481 cagctgcccg gcgcctacat cgtcggcatc aagttggaca tcacctccca caacgaggac
     3541 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     3601 gagctgtaca agtaaagatc taagccgaat tcgatatcaa gcttatcgat aatcaacctc
     3661 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3721 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3781 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     3841 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     3901 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     3961 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4021 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     4081 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4141 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4201 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     4261 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     4321 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     4381 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     4441 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     4501 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     4561 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     4621 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     4681 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     4741 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     4801 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4861 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4921 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     4981 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     5041 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     5101 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     5161 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     5221 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5281 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5341 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5401 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5461 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5521 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5581 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5641 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     5701 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     5761 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5821 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     5881 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5941 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6001 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     6061 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     6121 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     6181 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     6241 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     6301 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     6361 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     6421 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     6481 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     6541 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     6601 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     6661 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6721 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     6781 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6841 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6901 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6961 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7021 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7081 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7141 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7201 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7261 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7321 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7381 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7441 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7501 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7561 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7621 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7681 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7741 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7801 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7861 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7921 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7981 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8041 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8101 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8161 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8221 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8281 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8341 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8401 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8461 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8521 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8581 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8641 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8701 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8761 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8821 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8881 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8941 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     9001 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     9061 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     9121 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     9181 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     9241 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     9301 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     9361 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     9421 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     9481 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     9541 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     9601 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     9661 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     9721 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     9781 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9841 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9901 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9961 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
    10021 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
    10081 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    10141 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
    10201 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    10261 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    10321 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    10381 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    10441 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
    10501 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
    10561 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
    10621 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
    10681 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
    10741 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
    10801 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
    10861 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
    10921 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
    10981 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    11041 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    11101 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
    11161 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
    11221 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
    11281 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
    11341 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
    11401 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
    11461 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
    11521 tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc ac
//
