LOCUS       sequence-292246-        8578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Relocalization of genomically expressed protein of interest from 
            cytoplasm to nucleus upon near-infrared illumination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Redchuk TA, Karasev MM, Verkhusha PV, Donnelly SK, Hulsemann M, 
            Virtanen J, Moore HM, Vartiainen MK, Hodgson L, Verkhusha VV
  TITLE     Optogenetic regulation of endogenous proteins.
  JOURNAL   Nat Commun. 2020 Jan 30;11(1):605. doi: 10.1038/s41467-020-14460-4.
  PUBMED    32001718
REFERENCE   2  (bases 1 to 8578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-30"; volume: "11(1):605. doi"; pages: " 
            10.1038/s41467-020-14460-4"
COMMENT     Sequence Label: pQP-2363
FEATURES             Location/Qualifiers
     source          1..8578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        140..443
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        444..647
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     598..618
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      695..704
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2962..3303
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
     misc_feature    3313..3369
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3371..3945
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3395..3947
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3539..3556)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3766..3785
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4004..4711
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4149..4167)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4417..4436
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5216..5230
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     polyA_signal    5445..5566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5482..5501)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5536..5555
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5573..6028)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5710..5731)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5922..5941
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6055..6159
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6161..6518
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6161..6352
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6184..6204)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6369..6504
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6553..7347
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6553..7347
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6607..6626)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7217..7236
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7535..7554)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7579..7626
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7955..8543
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8444..8463
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
       61 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata
      121 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      181 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      241 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      301 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      361 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      421 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      481 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      541 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      601 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
      661 gtcagatccg ctagcgctac cggactcaga tctaccatgg tggcaggtca tgcctctggc
      721 agccccgcat tcgggaccgc cgatctttcg aattgcgaac gtgaagagat ccacctcgcc
      781 ggctcgatcc agccgcatgg cgcgcttctg gtcgtcagcg agccggatca tcgcatcatc
      841 caggccagcg ccaacgccgc ggaatttctg aatctcggaa gcgtgctcgg cgttccgctc
      901 gccgagatcg acggcgatct gttgatcaag atcctgccgc atctcgatcc caccgccgaa
      961 ggcatgccgg tcgcggtgcg ctgccggatc ggcaatccct ccacggagta cgacggtctg
     1021 atgcatcggc ctccggaagg cgggctgatc atcgagctcg aacgtgccgg cccgccgatc
     1081 gatctgtccg gcacgctggc gccggcgctg gagcggatcc gcacggcggg ctcgctgcgc
     1141 gcgctgtgcg atgacaccgc gctgctgttt cagcagtgca ccggctacga ccgggtgatg
     1201 gtgtatcgct tcgacgagca gggccacggc gaagtgttct ccgagcgcca cgtgcccggg
     1261 ctcgaatcct atttcggcaa ccgctatccg tcgtcggaca ttccgcagat ggcgcggcgg
     1321 ctgtacgagc ggcagcgcgt ccgcgtgctg gtcgacgtca gctatcagcc ggtgccgctg
     1381 gagccgcggc tgtcgccgct gaccgggcgc gatctcgaca tgtcgggctg cttcctgcgc
     1441 tcgatgtcgc cgatccatct gcagtacctg aagaacatgg gcgtgcgcgc caccctggtg
     1501 gtgtcgctgg tggtcggcgg caagctgtgg ggcctggttg cctgtcacca ttatctgccg
     1561 cgcttcatcc atttcgagct gcgggcgatc tgcgaactgc tcgccgaagc gatcgcgacg
     1621 cggatcaccg cgcttgagag cttcgcgcag agccagtcgg agctgttcgt gcagcggctc
     1681 gaacagcgca tgatcgaagc gatcacccgt gaaggcgatt ggcgcgcagc gattttcgac
     1741 accagccaat cgatcctgca gccgctgcac gccgacggtt gcgcgctggt gtacgaagac
     1801 cagatcagga ccatcggtga cgtaccttcc acgcaggatg ttcgcgagat cgccgggtgg
     1861 ctcgatcgcc agccacgtgc ggcggtgacc tcgaccgcgt cgctcggtct cgacgtgccg
     1921 gagctcgcgc atctgacgcg gatggcgagc ggcgtggtcg cggcgccgat ttcggatcat
     1981 cgcggcgagt ttctgatgtg gttccgcccc gagcgcgtcc acaccgttac ctggggcggc
     2041 gatccgaaga agccgttcac gatgggcgat acaccggcgg atctgtcgcc gcggcgctcc
     2101 ttcgccaaat ggcatcaggt tgtcgaaggc acgtccgatc cgtggacggc cgccgatctc
     2161 gccgcggctc gcaccatcgg tcagaccgtc gccgacatcg tgctgcaatt ccgcgcggtg
     2221 cggacactga tcgcccgcga acagtacgaa cagttttcgt cccaggtgca cgcttcgatg
     2281 cagccggtgc tgatcaccga cgccgaaggc cgcatcctgc tgatgaacga ctcgttccgc
     2341 gacatgttgc cggcggggtc gccatccgcc gtccatctcg acgatctcgc cgggttcttc
     2401 gtcgaatcga acgatttcct gcgcaacgtc gccgaactga tcgatcacgg ccgcgggtgg
     2461 cgcggcgaag ttctgctgcg cggcgcaggt aatcgcccgt tgccgctggc agtgcgcgcc
     2521 gatccggtga cgcgcacgga ggaccagtcg ctcggcttcg tgctgatctt cagcgacgct
     2581 accgatcgtc gcaccgcaga tgccgcacgc acgcgtttcc aggaaggcat tcttgccagc
     2641 gcacgtcccg gcgtgcggct cgactccaag tccgacctct tgcacgagaa gctgctgtcc
     2701 gcgctggtcg agaacgcgca gcttgccgca ttggaaatta cttacggcgt cgagaccgga
     2761 cgcatcgccg agctgctcga aggcgttcgc cagtcgatgc tgcgcaccgc cgaagtgctc
     2821 ggccatctgg tgcagcacgc ggcgcgcacg gccggcagcg acagctcgag caatggctcg
     2881 cagaacaaga aggctagcag tgctggcggt agtgctggtg ggagtgctgg cggtagtgct
     2941 ggtggcgata tcatggatca agtccaactg gtggagtctg gtggcgcttt ggtgcagcca
     3001 ggtggctctc tgcgtttgtc ctgtgccgct tctggcttcc cagtgaaccg ctattccatg
     3061 cgctggtatc gccaggctcc aggcaaagag cgtgagtggg tagccggtat gtccagcgcg
     3121 ggtgatcgta gctcctatga agactccgtg aagggccgtt tcaccatcag ccgtgacgat
     3181 gcccgtaaca cggtgtatct gcaaatgaac agcttgaaac ctgaagatac ggccgtgtat
     3241 tactgtaatg tgaacgtggg cttcgagtat tggggccaag gcacccaggt caccgtctcc
     3301 agctaagata tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
     3361 ccgggatccg cccctctccc tccccccccc cctaacgtta ctggccgaag ccgcttggaa
     3421 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     3481 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     3541 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     3601 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     3661 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     3721 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     3781 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     3841 gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     3901 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaaccg
     3961 gtccatggga attgatctct caggattaac actgcagact agtatggtga gcaagggcga
     4021 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     4081 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     4141 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     4201 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     4261 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     4321 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     4381 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     4441 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     4501 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     4561 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     4621 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     4681 ctccaccggc ggcatggacg agctgtacaa gagtgctggt gggtccgccg gcgggagtgc
     4741 tggcggtagt gccggcggta gttctagagg caagaacatg caggcggtca ccgagctgca
     4801 ttcccggctg atcgctgcgc agcaggcgat ggagcgcgac tattggcggt tgcgtgaatt
     4861 ggagactcgc taccgcctgg tgttcgacgc tgccgccgat gcggtgatga tcgtctccgc
     4921 cggcgacatg cgcatcgtcg aagccaaccg ggcggcggtg aatgcgatca gccgcgtcga
     4981 gcgcggcaat gacgaccttg cggggcgtga tttcctcgcc gaagtggcgg ctgccgatcg
     5041 cgatgcggtg cgcgacatgc tggcccaggt gcgtcagcgc ggcaccgcac tcagcgtcct
     5101 cgttcatctc ggccgttacg accgcgcctg gatgctgcgc ggttcgctga tgtcgtccga
     5161 gcgtcgtcag gttttcctgc tgcacttcac cccggtgacc acgactcccg cgatcgacga
     5221 cgacgacaaa ggtgtcgttg cttctgctgc cgacggtgca gagggcgcct cagacgacgc
     5281 agaggatgaa ttcatggctg ccaagagggt caagttggac tgagcggccg cgactctaga
     5341 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     5401 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     5461 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5521 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta
     5581 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     5641 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     5701 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     5761 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     5821 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     5881 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     5941 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     6001 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
     6061 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6121 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
     6181 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6241 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6301 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6361 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6421 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6481 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
     6541 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     6601 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     6661 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     6721 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     6781 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     6841 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     6901 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     6961 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     7021 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     7081 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     7141 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     7201 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     7261 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     7321 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     7381 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     7441 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     7501 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
     7561 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
     7621 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     7681 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     7741 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
     7801 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7861 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7921 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7981 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8041 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8101 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8161 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8221 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8281 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8341 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8401 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8461 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8521 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggc
//
