LOCUS       sequence-292248-       12140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12140)
  AUTHORS   Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas 
            E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM
  TITLE     mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP 
            Pathway.
  JOURNAL   Cell. 2011 Aug 5;146(3):408-20.
  PUBMED    21816276
REFERENCE   2  (bases 1 to 12140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-08-5"; volume: "146(3)"; pages: "408-20"
COMMENT     Sequence Label: pLKO-puro FLAG SREBP1
FEATURES             Location/Qualifiers
     source          1..12140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        288..514
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             515..695
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    742..867
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1364..1597
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3120..3164
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        3471..3674
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3625..3645
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3671..3695
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3905..3923
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3905..3922
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     3936..3955
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             4014..4037
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    7521..7638
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7687..8197
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8116..8134
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8219..8818
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8219..8238)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8715..8735
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8946..9179
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9251..9372
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9288..9307)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9342..9361
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9412..9547
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9567..9586)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9568..9586)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9596..9613)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9596..9612)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9605..9627)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9754..10209
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9841..9860)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10051..10072
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10235..10339
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10340..10408,10409..11200)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10558..10577)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11371..11959
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11860..11879
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12113..12130
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
      301 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
      361 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
      421 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
      481 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
      541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      661 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      721 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      781 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      841 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      901 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      961 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1021 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1081 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1141 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1201 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1261 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1321 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1381 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1441 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1501 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1561 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1621 aactcatttg caccactgct gtgccctgat gaatccccta atgattttta tcaaaatcat
     1681 taagttaagg tagatacaca tcttgtcata tgatcaaatg gtttcgccaa aaatcaataa
     1741 tcagacaaca aaatgtgcga actcgatatt ttacacgact ctctttacca attctgcccc
     1801 gaattacact taaaacgact caacagctta acgttggctt gccacgcctt acttgactgt
     1861 aaaactctca ctcttaccga acttggccgt aacctgccaa ccaaagcgag aacaaaacat
     1921 aacatcaaac gaatcgaccg attgttaggt aatcgtcacc tccacaaaga gcgactcgct
     1981 gtataccgtt ggcatgctag ctttatctgt tcgggcaata cgatgcccat tgtacttgtt
     2041 gactggtctg atatccgtga gcaaaaacgg cttatggtat tgcgagcttc agtcgcacta
     2101 cacggtcgtt ctgttactct ttatgagaaa gcgttcccgc tttcagagca atgttcaaag
     2161 aaagctcatg accaatttct agccgacctt gcgagcattc taccgagtaa caccacaccg
     2221 ctcattgtca gtgatgctgg ctttaaagtg ccatggtata aatccgttga gaagctgggt
     2281 tggtactggt taagtcgagt aagaggaaaa gtacaatatg cagacctagg agcggaaaac
     2341 tggaaaccta tcagcaactt acatgatatg tcatctagtc actcaaagac tttaggctat
     2401 aagaggctga ctaaaagcaa tccaatctca tgccaaattc tattgtataa atctcgctct
     2461 aaaggccgaa aaaatcagcg ctcgacacgg actcattgtc accacccgtc acctaaaatc
     2521 tactcagcgt cggcaaagga gccatggatt ctagcaacta acttacctgt tgaaattcga
     2581 acacccaaac aacttgttaa tatctattcg aagcgaatgc agattgaaga aaccttccga
     2641 gacttgaaaa gtcctgccta cggactaggc ctacgccata gccgaacgag cagctcagag
     2701 cgttttgata tcatgctgct aatcgccctg atgcttcaac taacatgttg gcttgcgggc
     2761 gttcatgctc agaaacaagg ttgggacaag cacttccagg ctaacacagt cagaaatcga
     2821 aacgtactct caacagttcg cttaggcatg gaagttttgc ggcattctgg ctacacaata
     2881 acaagggaag acttactcgt ggctgcaacc ctactagctc aaaatttatt cacacatggt
     2941 tacgctttgg ggaaattatg aggggatctc tcagtgctgt gccttggaat gctagttgga
     3001 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     3061 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     3121 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     3181 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     3241 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     3301 tatcgtttca gacccacctc ccaaccccga ggggacccca tatgccaagt acgcccccta
     3361 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     3421 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     3481 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     3541 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     3601 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     3661 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     3721 ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccgggaa cggtgcattg
     3781 gaacgcggat tccccgtgcc aagagtgacg taagtaccgc ctatagagtc tataggccca
     3841 cccccttggc ttcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca
     3901 tacgatttag gtgacactat agaataacat ccactttgcc tttctctcca caggtgtcca
     3961 ctcccaggtc caactgcacc tcggttctat cgattgaatt caccatggaa atggactaca
     4021 aggatgacga tgacaagggt ggtggtggtg gtgcgtcgac gatggacgag ctggccttcg
     4081 gtgaggcggc tctggaacag acactggccg agatgtgcga actggacaca gcggttttga
     4141 acgacatcga agacatgctc cagctcatca acaaccaaga cagtgacttc ccgggcctgt
     4201 ttgacgcccc ctatgctggg ggtgagacag gggacacagg ccccagcagc ccaggtgcca
     4261 actctcctga gagcttctct tctgcttctc tggcctcctc tctggaagcc ttcctgggag
     4321 gacccaaggt gacacctgca cccttgtccc ctccaccatc ggcacccgct gctttaaaga
     4381 tgtacccgtc cgtgtccccc ttttcccctg ggcctgggat caaagaggag ccagtgccac
     4441 tcaccatcct acagcctgca gcgccacagc cgtcaccggg gaccctcctg cctccgagct
     4501 tccccgcacc acccgtacag ctcagccctg cgcccgtgct gggttactcg agcctgcctt
     4561 caggcttctc agggaccctt ccaggaaaca ctcagcagcc accatctagc ctgccgctgg
     4621 cccctgcacc aggagtcttg cccacccctg ccctgcacac ccaggtccaa agcttggcct
     4681 cccagcagcc gctgccagcc tcagcagccc ctagaacaaa cactgtgacc tcacaggtcc
     4741 agcaggtccc agttgtactg cagccacact tcatcaaggc agactcactg ctgctgacag
     4801 ctgtgaagac agatgcagga gccaccatga agactgcagg catcagcacc ctggctcctg
     4861 gcacagccgt gcaggcaggt cccctgcaga ccctggtgag tggagggacc atcttggcca
     4921 cagtaccttt ggttgtggac acagacaaac tgcccatcca ccgactcgca gctggcagca
     4981 aggccctagg ctcagctcag agccgtggtg agaagcgcac agcccacaat gccattgaga
     5041 agcgctaccg gtcctctatc aatgacaaga ttgtggagct caaagacctg gtggtgggca
     5101 ctgaagcaaa gctgaataaa tctgctgtct tgcgcaaggc catcgactac atccgcttct
     5161 tgcagcacag caaccagaag ctcaagcagg agaacctgac cctacgaagt gcacacaaaa
     5221 gcaaatcact gaaggacctg gtgtcagctt gtggcagtgg aggaggcaca gatgtgtcta
     5281 tggagggcat gaaacccgaa gtggtggaga cgcttacccc tccaccctca gacgccggct
     5341 caccctccca gagtagcccc ttgtcttttg gcagcagagc tagcagcagt ggtggtagtg
     5401 actctgagcc cgacagtcca gcctttgagg atagccaggt caaagcccag cggctgcctt
     5461 cacacagccg aggcatgctg gaccgctccc gcctggccct gtgtgtactg gcctttctgt
     5521 gtctgacctg caatcctttg gcctcgcttt tcggctgggg cattctcact ccctctgatg
     5581 ctacgggtac acaccgtagt tctgggcgca gcatgctgga ggcagagagc agagatggct
     5641 ctaattggac ccagtggttg ctgccacccc tagtctggct ggccaatgga ctactagtgt
     5701 tggcctgctt ggctcttctc tttgtctatg gggaacctgt gactaggcca cactctggcc
     5761 cggctgtaca cttctggaga catcgcaaac aagctgacct ggatttggcc cggggagatt
     5821 tcccccaggc tgctcaacag ctgtggctgg ccctgcaagc gctgggccgg cccctgccca
     5881 cctcaaacct ggatctggcc tgcagtctgc tttggaacct catccgccac ctgctccagc
     5941 gtctctgggt gggccgctgg ctggcaggcc aggccggggg cctgctgagg gaccgtgggc
     6001 tgaggaagga tgcccgtgcc agtgcccggg atgcggctgt tgtctaccat aagctgcacc
     6061 agctgcatgc catgggcaag tacacaggag gacatcttgc tgcttctaac ctggcactaa
     6121 gtgccctcaa cctggctgag tgcgcaggag atgctatctc catggcaaca ctggcagaga
     6181 tctatgtggc agcggccctg agggtcaaaa ccagcctccc aagagccctg cacttcttga
     6241 cacgtttctt cctgagcagc gcccgccagg cctgcctagc acagagcggc tcggtgcctc
     6301 ttgccatgca gtggctctgc caccctgtag gtcaccgttt ctttgtggac ggggactggg
     6361 ccgtgcacgg tgcccccccg gagagcctgt acagcgtggc tgggaaccca gtggatccgc
     6421 tggcccaggt gacccggcta ttccgtgaac atctcctaga gcgagcgttg aactgtattg
     6481 ctcagcccag cccaggggca gctgacggag acagggagtt ctcagatgcc cttggatatc
     6541 tgcagttgct aaatagctgt tctgatgctg ccggggctcc tgcttgcagt ttctctgtca
     6601 gctccagcat ggctgccacc actggcccag acccagtggc caagtggtgg gcctcactga
     6661 cagctgtggt gatccactgg ctgaggcggg atgaagaggc agctgagcgc ttgtacccac
     6721 tggtagagca tatcccccag gtgctgcagg acactgagag acccctgccc agggcagctc
     6781 tgtactcctt caaggctgcc cgggctctgc tggaccacag aaaggtggaa tctagcccag
     6841 ccagcctggc catctgtgag aaggccagtg ggtacctgcg ggacagctta gcctctacac
     6901 caactggcag ttccattgac aaggccatgc agctgctcct gtgtgatcta cttcttgtgg
     6961 cccgtaccag tctgtggcag cggcagcagt caccagcttc agtccaggta gctcacggta
     7021 ccagcaatgg accccaggcc tctgctctgg agctgcgtgg tttccaacat gacctgagca
     7081 gcctgcggcg gttggcacag agcttccggc ctgctatgag gagggtattc ctacatgagg
     7141 ccacagctcg gctgatggca ggagcaagtc ctgcccggac acaccagctc ctggatcgca
     7201 gtctgaggag gagggcaggt tccagtggca aaggaggcac tacagctgag ctggagccac
     7261 ggcccacatg gcgggagcac accgaggccc tgctgttggc atcctgctat ctgccccctg
     7321 ccttcctgtc ggctcctggg cagcgaatga gcatgctggc cgaggcggca cgcaccgtag
     7381 agaagcttgg cgatcaccgg ctactgctgg actgccagca gatgctcctg cgcctgggcg
     7441 gcggaaccac cgtcacttcc agctaggcgg ccgcagtgag aattctcgac ctcgagacaa
     7501 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     7561 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     7621 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg
     7681 ctcgaggggg ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg
     7741 ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt
     7801 cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac
     7861 gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg
     7921 acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat
     7981 ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga
     8041 gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct
     8101 gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg
     8161 ctccctcgtt gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccat
     8221 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     8281 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     8341 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     8401 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     8461 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     8521 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     8581 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     8641 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     8701 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     8761 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     8821 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggtacc
     8881 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8941 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     9001 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9061 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9121 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9181 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     9241 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9301 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9361 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     9421 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     9481 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     9541 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     9601 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     9661 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     9721 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     9781 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     9841 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     9901 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     9961 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
    10021 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
    10081 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
    10141 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
    10201 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
    10261 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
    10321 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
    10381 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
    10441 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
    10501 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
    10561 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
    10621 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
    10681 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    10741 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    10801 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    10861 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
    10921 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    10981 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    11041 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    11101 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    11161 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    11221 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    11281 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    11341 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    11401 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    11461 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    11521 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    11581 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    11641 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    11701 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    11761 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    11821 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    11881 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    11941 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    12001 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    12061 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    12121 agtgagcgag gaagcggaag
//
