LOCUS       sequence-292251-        9064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Light-controlled tri-directional targeting of endogenous proteins 
            (nucleus-shifted).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Redchuk TA, Karasev MM, Verkhusha PV, Donnelly SK, Hulsemann M, 
            Virtanen J, Moore HM, Vartiainen MK, Hodgson L, Verkhusha VV
  TITLE     Optogenetic regulation of endogenous proteins.
  JOURNAL   Nat Commun. 2020 Jan 30;11(1):605. doi: 10.1038/s41467-020-14460-4.
  PUBMED    32001718
REFERENCE   2  (bases 1 to 9064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-30"; volume: "11(1):605. doi"; pages: " 
            10.1038/s41467-020-14460-4"
COMMENT     Sequence Label: pQP-NiRISB
FEATURES             Location/Qualifiers
     source          1..9064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      616..625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2910..2966
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    2968..3542
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2992..3544
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3136..3153)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3363..3382
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3613..3954
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
     CDS             3976..4683
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4121..4139)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4389..4408
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5188..5202
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     polyA_signal    5852..5973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5889..5908)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5943..5962
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5980..6435)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6117..6138)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6329..6348
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6462..6566
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6568..6925
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6568..6759
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6591..6611)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6776..6911
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6960..7754
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6960..7754
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7014..7033)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7624..7643
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7942..7961)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7986..8033
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8362..8950
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8851..8870
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctaccatg gtggcaggtc atgcctctgg cagccccgca ttcgggaccg
      661 ccgatctttc gaattgcgaa cgtgaagaga tccacctcgc cggctcgatc cagccgcatg
      721 gcgcgcttct ggtcgtcagc gagccggatc atcgcatcat ccaggccagc gccaacgccg
      781 cggaatttct gaatctcgga agcgtgctcg gcgttccgct cgccgagatc gacggcgatc
      841 tgttgatcaa gatcctgccg catctcgatc ccaccgccga aggcatgccg gtcgcggtgc
      901 gctgccggat cggcaatccc tccacggagt acgacggtct gatgcatcgg cctccggaag
      961 gcgggctgat catcgagctc gaacgtgccg gcccgccgat cgatctgtcc ggcacgctgg
     1021 cgccggcgct ggagcggatc cgcacggcgg gctcgctgcg cgcgctgtgc gatgacaccg
     1081 cgctgctgtt tcagcagtgc accggctacg accgggtgat ggtgtatcgc ttcgacgagc
     1141 agggccacgg cgaagtgttc tccgagcgcc acgtgcccgg gctcgaatcc tatttcggca
     1201 accgctatcc gtcgtcggac attccgcaga tggcgcggcg gctgtacgag cggcagcgcg
     1261 tccgcgtgct ggtcgacgtc agctatcagc cggtgccgct ggagccgcgg ctgtcgccgc
     1321 tgaccgggcg cgatctcgac atgtcgggct gcttcctgcg ctcgatgtcg ccgatccatc
     1381 tgcagtacct gaagaacatg ggcgtgcgcg ccaccctggt ggtgtcgctg gtggtcggcg
     1441 gcaagctgtg gggcctggtt gcctgtcacc attatctgcc gcgcttcatc catttcgagc
     1501 tgcgggcgat ctgcgaactg ctcgccgaag cgatcgcgac gcggatcacc gcgcttgaga
     1561 gcttcgcgca gagccagtcg gagctgttcg tgcagcggct cgaacagcgc atgatcgaag
     1621 cgatcacccg tgaaggcgat tggcgcgcag cgattttcga caccagccaa tcgatcctgc
     1681 agccgctgca cgccgacggt tgcgcgctgg tgtacgaaga ccagatcagg accatcggtg
     1741 acgtaccttc cacgcaggat gttcgcgaga tcgccgggtg gctcgatcgc cagccacgtg
     1801 cggcggtgac ctcgaccgcg tcgctcggtc tcgacgtgcc ggagctcgcg catctgacgc
     1861 ggatggcgag cggcgtggtc gcggcgccga tttcggatca tcgcggcgag tttctgatgt
     1921 ggttccgccc cgagcgcgtc cacaccgtta cctggggcgg cgatccgaag aagccgttca
     1981 cgatgggcga tacaccggcg gatctgtcgc cgcggcgctc cttcgccaaa tggcatcagg
     2041 ttgtcgaagg cacgtccgat ccgtggacgg ccgccgatct cgccgcggct cgcaccatcg
     2101 gtcagaccgt cgccgacatc gtgctgcaat tccgcgcggt gcggacactg atcgcccgcg
     2161 aacagtacga acagttttcg tcccaggtgc acgcttcgat gcagccggtg ctgatcaccg
     2221 acgccgaagg ccgcatcctg ctgatgaacg actcgttccg cgacatgttg ccggcggggt
     2281 cgccatccgc cgtccatctc gacgatctcg ccgggttctt cgtcgaatcg aacgatttcc
     2341 tgcgcaacgt cgccgaactg atcgatcacg gccgcgggtg gcgcggcgaa gttctgctgc
     2401 gcggcgcagg taatcgcccg ttgccgctgg cagtgcgcgc cgatccggtg acgcgcacgg
     2461 aggaccagtc gctcggcttc gtgctgatct tcagcgacgc taccgatcgt cgcaccgcag
     2521 atgccgcacg cacgcgtttc caggaaggca ttcttgccag cgcacgtccc ggcgtgcggc
     2581 tcgactccaa gtccgacctc ttgcacgaga agctgctgtc cgcgctggtc gagaacgcgc
     2641 agcttgccgc attggaaatt acttacggcg tcgagaccgg acgcatcgcc gagctgctcg
     2701 aaggcgttcg ccagtcgatg ctgcgcaccg ccgaagtgct cggccatctg gtgcagcacg
     2761 cggcgcgcac ggccggcagc gacagctcga gcaatggctc gcagaacaag aaggctagca
     2821 gtgctggcgg tagtgctggt agtgctggtc gtacggccag cggtaaaaag aagaaaaaga
     2881 agtcaaagac aaagtgtgta attatgtaaa gatctcgagc tcaagcttcg aattctgcag
     2941 tcgacggtac cgcgggcccg ggatccgccc ctctccctcc ccccccccct aacgttactg
     3001 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     3061 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     3121 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     3181 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     3241 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     3301 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     3361 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     3421 gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag tcgaggttaa
     3481 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     3541 aatatggcca caaaccggtc catgggaatt gatctctcag gattaacact gcagactagc
     3601 gccatgggcc aagtccaact ggtggagtct ggtggcgctt tggtgcagcc aggtggctct
     3661 ctgcgtttgt cctgtgccgc ttctggcttc ccagtgaacc gctattccat gcgctggtat
     3721 cgccaggctc caggcaaaga gcgtgagtgg gtagccggta tgtccagcgc gggtgatcgt
     3781 agctcctatg aagactccgt gaagggccgt ttcaccatca gccgtgacga tgcccgtaac
     3841 acggtgtatc tgcaaatgaa cagcttgaaa cctgaagata cggccgtgta ttactgtaat
     3901 gtgaacgtgg gcttcgagta ttggggccaa ggcacccagg tcaccgtctc cagcggaggc
     3961 ggatcatcta ctagtatggt gagcaagggc gaggaggata acatggccat catcaaggag
     4021 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     4081 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     4141 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     4201 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     4261 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     4321 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     4381 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     4441 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     4501 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     4561 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     4621 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     4681 aagagtgctg gtgggtccgc cggcgggagt gctggcggta gtgccggcgg tagttctaga
     4741 ggcaagaaca tgcaggcggt caccgagctg cattcccggc tgatcgctgc gcagcaggcg
     4801 atggagcgcg actattggcg gttgcgtgaa ttggagactc gctaccgcct ggtgttcgac
     4861 gctgccgccg atgcggtgat gatcgtctcc gccggcgaca tgcgcatcgt cgaagccaac
     4921 cgggcggcgg tgaatgcgat cagccgcgtc gagcgcggca atgacgacct tgcggggcgt
     4981 gatttcctcg ccgaagtggc ggctgccgat cgcgatgcgg tgcgcgacat gctggcccag
     5041 gtgcgtcagc gcggcaccgc actcagcgtc ctcgttcatc tcggccgtta cgaccgcgcc
     5101 tggatgctgc gcggttcgct gatgtcgtcc gagcgtcgtc aggttttcct gctgcacttc
     5161 accccggtga ccacgactcc cgcgatcgac gacgacgaca aaggtgtcgt tgcttctgct
     5221 gccgacggtg cagagggcgc ctcagacgac gcagaggatg aattcccggc tagcggcgcc
     5281 ggcgcctttt tggctactac acttgaacgt attgagaaga actttgtcat tactgaccca
     5341 aggttgccag ataatcccat tatattcgcg tccgatagtt tcttgcagtt gacagaatat
     5401 agccgtgaag aaattttggg aagaaactgc aggtttctac aaggtcctga aactgatcgc
     5461 gcgacagtga gaaaaattag agatgccata gataaccaaa cagaggtcac tgttcagctg
     5521 attaattata caaagagtgg taaaaagttc tggaacctct ttcacttgca gcctatgcga
     5581 gatcagaagg gagatgtcca gtactttatt ggggttcagt tggatggaac tgagcatgtc
     5641 cgagatgctg ccgagagaga gggcgttatg ctggccaaga agaccgccga gaacatcgac
     5701 atggctgcca agagggtcaa gttggactga gcggccgcga ctctagatca taatcagcca
     5761 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     5821 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     5881 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5941 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
     6001 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     6061 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     6121 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     6181 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     6241 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     6301 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     6361 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     6421 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6481 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6541 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     6601 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6661 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6721 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6781 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6841 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6901 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     6961 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     7021 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     7081 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     7141 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     7201 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     7261 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     7321 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     7381 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     7441 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     7501 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     7561 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     7621 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     7681 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     7741 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     7801 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7861 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7921 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     7981 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     8041 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     8101 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     8161 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     8221 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8281 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8341 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8401 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8461 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8521 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8581 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8641 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8701 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8761 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8821 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8881 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8941 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9001 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
     9061 gcat
//
